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Borrelia burgdorferi spirochetes are an infectious agent of Lyme borreliosis. The aim of our studies was to investigate the fate of engulfed B. burgdorferi cells in L-929 mouse fibroblasts and to observe development of intracellular infection in vitro after 2 and 48 h. Electron microscopic studies reveal consecutive stages of B. burgdorferi spirochetes penetration to mouse fibroblasts in vitro. It has been observed, as a first step attachment and engulfment of spirochetes followed by formation of vacuoles. After 48 hours of infection, vacuoles of fibroblastic cells have been seen full of B. burgdorferi bacteria and latter they have been released from infected cells to extracellular space. It can be the evidence that B. burgdorferi multiply intracellulary.
Q fever is an infectious zoonotic disease characterized by sudden fever, headache, and atypical pneumonia, caused by Coxiella burnetii – an obligatory intracellular parasite. Based on phylogenetic analysis of the genes sequences, the classification was changed and C. burnetii species was included to the gamma subgroup of the proteobacteria, Legionellales order and Coxiellaceae family. This analysis showed more than 99% sequence similarity of 16SrRNA gene among the strains isolated in different regions of the world. Q fever is a widespread in the world zoonosis. Its main reservoir in the rural environment are farm animals: cows, sheep, goats, and urban pets such as dogs, cats, rabbits. In acute infection these bacteria are detected in various internal organs such as lungs, liver, spleen, and in excretion in urine, faeces and milk. During childbirth, they occur in large number in the amniotic fluid and placenta. Recently, it has been found that free-living amoeba Acanthamoeba castellani may also be a reservoir of the pathogen. The intra-amoebal location of C. burnetii cells was observed.
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Tick-borne bacterial diseases in Poland

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Many infectious diseases are spread by a vector. Some microorganisms require both the vertebrate as well invertebrate host to complete their life cycle. In this way, many bacterial diseases are transmitted to humans. Within Borrelia burgdorferi sensu lato species, 15 genospecies have been identified; 7 of which are pathogenic to humans. Lyme borreliosis, classified as a zoonosis, occurs throughout the Northern Hemisphere. The symptoms caused by these spirochetes, in addition to the characteristic erythema migrans, may affect many body systems and organs. Infections caused by Bartonella spp. are classified as emerging and re-emerging diseases. Over 25 species of Bartonella species have been currently recognized, 14 of which are pathogenic to humans. The infections of these microorganisms are transmitted by ticks, lice and fleas, and manifest themselves as endocarditis, meningitis, pneumonia, bacillary peliosis, Parinaud’s oculoglandular syndrome, the mildest of them being the cat scratch disease. Recently, spotted fever group rickettsioses are the most commonly recognized. In Poland, the etiological agents of these diseases are various species of Rickettsia spp., such as R. helvetica, R. monacensis, R. slovaca and R. raoultii. Human granulocytic anaplasmosis (HGA) is an acute infectious disease caused by Anaplasma phagocytophilum. The symptoms of anaplasmosis include: a high fever, headache, malaise, muscle pains and chills. It is characterised by thrombocytopenia, leukopenia, elevated levels of liver transaminases, increased number of neutrophils and mild anemia. There is no specific prophylaxis to avert tick-borne infections. The best prevention method is to avoid tick bites and to remove them from the skin immediately. It is also recommended to use tick repellents.
Apoptosis is a genetically controlled mechanism of cell death involved in the regulation of tissue homeostasis. The aim of this study was to investigate the influence of Borrelia afzelii, Coxiella burnetii, and Bartonella henselae bacteria on apoptosis measured as the level of caspase 3 activity in human fibroblast cells HEL-299. Our findings show that C. burnetii bacteria may inhibit the process of apoptosis in the host cells for a long time. This can permit intracellular survival in the host and mediatingthe development of chronic disease.
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