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Molecular mapping of powdery mildew resistance gene Eg-3 in cultivated oat (Avena sativa L. cv. Rollo)

100%

Mohler V., Zeller F. J., Hsam S. L. K.

Journal of Applied Genetics

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2012

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tom 53

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nr 2

Powdery mildew is a prevalent fungal disease affecting oat (Avena sativa L.) production in Europe. Common oat cultivar Rollo was previously shown to carry the powdery mildew resistance gene Eg-3 in common with cultivar Mostyn. The resistance gene was mapped with restriction fragment length polymorphism (RFLP) markers from Triticeae group-1 chromosomes using a population of F3 lines from a cross between A. byzantina cv. Kanota and A. sativa cv. Rollo. This comparative mapping approach positioned Eg-3 between cDNA-RFLP marker loci cmwg706 and cmwg733. Since both marker loci were derived from the long arm of barley chromosome 1H, the subchromosomal location of Eg-3 was assumed to be on the long arm of oat chromosome 17. Amplified fragment length polymorphism (AFLP) marker technology featured as an efficient means for obtaining markers closely linked to Eg-3.

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The genetics of resistance to powdery mildew in cultivated oats (Avena sativa L.): current status of major genes

100%

Hsam S. L. K., Mohler V., Zeller F. J.

Journal of Applied Genetics

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2014

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tom 55

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nr 2

3

Characterization of powdery mildew resistance and linkage studies involving the Pm3 locus on chromosome 1A of common wheat (Triticum aestivum L.)

100%

Hsam N. B. O., Kowalczyk K., Zeller F. J., Hsam S. L. K.

Journal of Applied Genetics

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2015

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tom 56

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nr 1

4

Identyfikacja genu odpornosci na rdze zolta Yr9 za pomoca testu zywiciel-patogen i markerow DNA w polskich odmianach pszenicy zwyczajnej

100%

Kowalczyk K., Hsam S. L. K., Zeller F. J.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2007

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tom 517

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nr 1

439-446

W pracy inokulowano dwoma izolatami rdzy żółtej (Puccinia striiformis Westend f. sp. tritici) GR2 i GR5, 70 polskich odmian pszenicy zwyczajnej. Jako formy kontrolne zastosowano odmiany: Vuka (podatna na porażenie) oraz Disponent zawierającą geny Yr9. Na podstawie przeprowadzonych badań wykazano, że odmiany: Aleta, Jubilatka, Lama, Lanca, Olma, Weneda, Wilga oraz odmiana kontrolna Disponent były odporne na porażenie przez izolaty GR2 i GR5. Za pomocą markerów STS-PCR stwierdzono obecność translokacji 1BL/1RS w tych odmianach. Na podstawie przeprowadzonych badań wykazano, że polskie odmiany pszenicy zwyczajnej: Aleta, Jubilatka, Lama, Lanca, Olma, Weneda oraz Wilga zawierają geny odporności na rdzę żółtą Yr9.

5

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Identification of powdery mildew resistance genes in common wheat [Triticum aestivum L.em.Thell.]. XI. Cultivars grown in Poland

100%

Kowalczyk K., Hsam S. L. K., Zeller F. J.

Journal of Applied Genetics

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1998

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tom 39

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nr 3

A collection of common wheat cullivars grown in Poland were analyzed for resistance to powdery mildew disease by using eleven differential isolates of Erysiphe graminis f. sp. tritici (Blumeria graminis). Among a total of 69 accessions, 48 cultivars possessed resistance which is attributed to known resistance genes present either individually or in a combination. Four cultivars were resistant to all the isolates used and another four cultivars revealed race-specific resistance which does not correspond to the response patterns of previously documented resistance. Resistance genes Pm2 and Pm6 in a combination were most widely distributed, and genes Pm3d, Pm4b, Pm5 and Pm8 were also postulated.

6

Identification of powdery mildew resistance genes in common wheat [Triticum aestivum L.em.Thell.]. X. Cultivars grown in Belarus and neighbouring countries

100%

Gordel S. I., Hsam S. L. K., Zeller F. J.

Journal of Applied Genetics

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1998

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tom 39

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nr 1

Sixty-six wheat cultivars grown in Belarus, Poland, Russia and the Ukraine were tested for mildew response to a collection of 11 different isolates of Erysiphe graminis DC f. sp. tritici Marchal. Nineteen cultivars have shown a susceptible reaction and eighteen were characterized by susceptible or intermediate responses. Fourteen cultivars revealed isolate-specific response patterns that could be attributed to major known resistance genes or gene combinations. Twelve cultivars have one documented gene: Pm5 in eight cultivars, Pm2 in two cultivars and Pm8 also in two cultivars. One cultivar has two genes (Pm2 + Pm6), while another cultivar carries a combination of three genes (Pm1 + Pm2 + Pm6). Fifteen cultivars were characterized by response patterns not documented so far or by a known resistance response combined with an undocumented resistance. Apparently three cultivars with the T1BL.1RS wheat-rye translocation have a gene suppressing the Pm8 mildew resistance. One cultivar was resistant to all the used isolates. Its resistance might be conditioned by an unknown major gene or combination of genes.

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Chromosomal location and molecular mapping of a tan spot resistance gene in the winter wheat cultivar Red Chief

76%

Tadesse W., Schmolke M., Hsam S. L. K., Mohler V., Wenzel G., Zeller F. J.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

The winter wheat cultivar Red Chief has been identified as the wheat cultivar most resistant to Pyrenophora tritici-repentis (Ptr). This study was undertaken to determine the inheritance, chromosomal location and molecular mapping of a tan spot resistance gene in Red Chief, χ² analysis of the F₂ segregation data of the hybrids between 21 monosomic lines of the susceptible wheat cultivar Chinese Spring and the resistant cultivar Red Chief revealed that tan spot resistance in cv. Red Chief is controlled by a single recessive gene located on chromosome 3 A. Linkage analysis using SSR markers in the Red Chief/Chinese Spring F₂ population showed that the tsr4 gene is clustered in the region around Xgwm 2a, on the short arm of chromosome 3 A. This marker has also been identified as the closest marker to the tsr3 locus on chromosome 3D in synthetic wheat lines. Validation analysis of this marker for the tsr3 and tsr4 genes using 28 resistant and 6 susceptible genotypes indicated that the 120 bp allele (the tsr3 gene) specific fragment was observed in 11 resistant genotypes, including the three synthetic lines XX41, XX45 and XX110, while the 130 bp allele was amplified only in cv. Red Chief and Dashen. Xgwm2a can be used to trace the presence of the target gene in successive backcross generations and pyramiding of the tsr3 & tsr4 genes into a commonly grown and adaptable cultivar.

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Cloning, expression, and evolutionary analysis of alpha-gliadin genes from Triticum and Aegilops genomes

64%

Li J., Wang S.-L., Cao M., Lv D.-W., Subburaj S., Li X.-H., Zeller F. J., Hsam S. L. K., Yan Y.-M.

Journal of Applied Genetics

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2013

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tom 54

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nr 2

Fifteen novel α-gliadin genes were cloned and sequenced from Triticum and related Aegilops genomes by allele-specific polymerase chain reaction (AS-PCR). Sequence comparison displayed high diversities in the α-gliadin gene family. Four toxic epitopes and glutamine residues in the two polyglutamine domains facilitated these α-gliadins to be assigned to specific chromosomes. Five representative α-gliadin genes were successfully expressed in Escherichia coli, and their amount reached a maximum after 4 h induced by isopropyl-β-D-thiogalactoside (IPTG), indicating a high level of expression under the control of T7 promoter. The transcriptional expression of α-gliadin genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed a similar up– down regulation pattern in different genotypes. A neighborjoining tree constructed with both full-open reading frame (ORF) α-gliadin genes and pseudogenes further revealed the origin and phylogenetic relationships among Triticum and related Aegilops genomes. The evolutionary analysis demonstrated that α-gliadin genes evolved mainly by synonymous substitutions under strong purifying selection during the evolutionary process.

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Molecular characterisation and evolution of HMW glutenin subunit genes in Brachypodium distachyon L.

64%

Subburaj S., Chen G., Han C., Lv D., Li X., Zeller F. J., Hsam S. L. K., Yan Y.

Journal of Applied Genetics

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2014

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tom 55

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nr 1

Ograniczanie wyników

Molecular mapping of powdery mildew resistance gene Eg-3 in cultivated oat (Avena sativa L. cv. Rollo)

The genetics of resistance to powdery mildew in cultivated oats (Avena sativa L.): current status of major genes

Characterization of powdery mildew resistance and linkage studies involving the Pm3 locus on chromosome 1A of common wheat (Triticum aestivum L.)

Identyfikacja genu odpornosci na rdze zolta Yr9 za pomoca testu zywiciel-patogen i markerow DNA w polskich odmianach pszenicy zwyczajnej

Identification of powdery mildew resistance genes in common wheat [Triticum aestivum L.em.Thell.]. XI. Cultivars grown in Poland

Identification of powdery mildew resistance genes in common wheat [Triticum aestivum L.em.Thell.]. X. Cultivars grown in Belarus and neighbouring countries

Chromosomal location and molecular mapping of a tan spot resistance gene in the winter wheat cultivar Red Chief

Cloning, expression, and evolutionary analysis of alpha-gliadin genes from Triticum and Aegilops genomes

Molecular characterisation and evolution of HMW glutenin subunit genes in Brachypodium distachyon L.