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Differential expression of expressed sequence tags in alfalfa roots under aluminium stresse

100%

Fan F., Li X.-W., Wu Y.-M., Xia Z.-S., Li J.-J., Zhu W., Liu J.-X.

Acta Physiologiae Plantarum

2011

tom 33

nr 2

To gain a better understanding of differentially expressed sequence tags (ESTs) for aluminum (Al) tolerance and to investigate the molecular mechanisms of Al toxicity, cDNA subtraction libraries were generated from Al-stressed roots of alfalfa (Medicago sativa L.) compared with no Al-stressed ones, employing suppression subtractive hybridization. Using differential screening technique in which the probes were labeled with DIG, we identified 45 non-redundant ESTs in Al-stressed alfalfa root tips with significantly altered expression. Among the up-regulated ESTs, we have found genes encoding identified proteins, including malate dehydrogenase, 6-phosphogluconate dehydrogenase, peroxidase, and an ABC transporter, while the down-regulate genes included ATPase, secretory carrier membrane protein 2, pectinesterase inhibitor. In addition, two novel ESTs, EW678752 and EY976957, up- and down-regulated by Al stress were sequenced. Analyzed by real-time PCR, the expressions of EST EW678718, EW678739, EY976969 and EW678728, which encode for ABC transporter, malate dehydrogenase, peroxidase and 6-phosphogluconate dehydrogenase correspondingly, increased 1.64-, 2.75-, 3.27- and 6.54-folds, respectively, and the expression of EY976957 encoding for ATPase decreased 3.27 folds. The expression of EST EW678752 increased 34.54-fold, while that of EY976957 decreased 16.68 folds. It suggested that the two novel ESTs maybe play a significant role in the aluminum tolerance of alfalfa.

TCP family genes control leaf development and its responses to gibberellin in celery

100%

Duan A.-Q., Wang Y.-W., Feng K., Liu J.-X., Xu Z.-S., Xiong A.-S.

Acta Physiologiae Plantarum

2019

tom 41

nr 09

Celery is a biennial herb of the Apiaceae family and is a leafy vegetable crop widely cultivated worldwide. TCP (teosinte branched1/cycloidea/proliferating cell factors), a transcription factor family, is involved in cell growth and leaf tissue proliferation. In this study, 32 AgTCP transcription factors were identified and analyzed based on the celery transcriptome and genome database and divided into two classes. Analysis of structural feature, phylogenetic tree, interaction network, and subcellular localization of AgTCP proteins was performed. It is shown that the AgTCP2 protein was positioned in the nucleus, and TCP proteins in the same group had higher similarity. Heatmap clusters of AgTCP genes expression levels during different celery leaf developmental stages suggested that the genes from the same evolutionary branch tended to form a cluster. The expression profiles of four AgTCP genes were detected at celery leaf developmental stages and under gibberellin (GA₃) treatment. As a CYC-type gene, AgTCP4 showed significantly increased expression levels under developmental stage and GA₃ treatment in ‘Liuhe Huangxinqin’. The results of this work give potential helpful information for further analysis of the role of AgTCP transcription factors in leaf development and hormone regulation in celery.

Isolation and characterization of pyrroline-5-carboxylate synthetase gene from perennial ryegrass (Lolium perenne L.)

84%

Cao L., Han L., Zhang H.-I., Xin X.-b., Imtiaz M., Yi M.-F., Sun Z.-Y., Ju G.-S., Qian Y.-Q., Liu J.-X.

Acta Physiologiae Plantarum

2015

tom 37

nr 03

Perennial ryegrass is an important turf grass and also used as a forage plant. However, abiotic stresses such as salt and drought are main limitations to its cultivation. In the present work, we cloned the gene encoding pyrroline-5-carboxylate synthetase (P5CS) from Lolium perenne, which is responsible for proline biosynthesis. This gene had a coding sequence (CDS) of 2151 base pairs (bp) encoding 716 amino acids. Multi-alignment analyses showed that the putative Lolium perenne P5CS (LpP5CS) contain all conserved functional sites and regions, and also displayed considerable similarities to Triticum astevum P5CS (TaP5CS) and Oryza sativa P5CSs (OsP5CSs). The real-time polymerase chain reaction (PCR) showed that the LpP5CS was highly expressed in leaves than in other tissues under normal conditions, and induced by sodium chloride (NaCl), abscisic acid (ABA), polyethylene glycol and cold treatments. Furthermore, ectopic expression of LpP5CS led to proline accumulation in tobacco under normal conditions. The transgenic tobacco over-expressing LpP5CS exhibited stronger tolerance to salt and drought as compared to control. These results showed, that LpP5CS responds to stress signals involving salt, drought, cold and ABA in perennial ryegrass. Our data indicate that LpP5CS might be a candidate gene for stressassociated molecular breeding in perennial ryegrass.

Ograniczanie wyników

3 Acta Physiologiae Plantarum

3 Liu J.-X.

1 Cao L.

1 Duan A.-Q.

1 Fan F.

1 Feng K.

1 Han L.

1 Imtiaz M.

1 Ju G.-S.

1 Li J.-J.

1 Li X.-W.

1 Qian Y.-Q.

1 Sun Z.-Y.

1 Wang Y.-W.

1 Wu Y.-M.

1 Xia Z.-S.

1 Xin X.-b.

1 Xiong A.-S.

1 Xu Z.-S.

1 Yi M.-F.

1 Zhang H.-I.

1 Zhu W.

1 2019

1 2015

1 2011

Wyniki wyszukiwania

Differential expression of expressed sequence tags in alfalfa roots under aluminium stresse

TCP family genes control leaf development and its responses to gibberellin in celery

Isolation and characterization of pyrroline-5-carboxylate synthetase gene from perennial ryegrass (Lolium perenne L.)