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Establishing an efficient explant superficial sterilization protocol for in vitro micropropagation of bear’s garlic (Allium ursinum L.)

100%

Tomaszewska-Sowa M., Figas A., Keutgen N., Keutgen

Herba Polonica

2015

tom 61

nr 4

Allium ursinum L. has a commercial value due to its high contents of bioactive compounds and mild, garlic-like taste. In vitro culture played an important role in obtaining Allium species with the desired characteristics and in the production of healthy reproductive material. Developing an effective method of sterilization bear’s garlic bulbs. To obtain sterile shoots of garlic several methods of sterilization involving such factors as ACE, H2O2, HgCl2, and UV-C were tested. In order to obtain sterile shoots of bear’s garlic, several sterilization procedures were tested. The best procedure was based on a two-step disinfection, where the whole onions were treated with ethanol and H2O2 for 20 min. Thereafter, the isolated apical buds were sterilized in ACE for 10 min, rinsed in double-distilled water and transferred onto MS medium for growing. Up to 95% of the inoculated explants formed shoots, which were sub-cultured on MS with 4 mg dm-3 BAP in order to enable further propagation. After optimization and stabilization, this procedure may become the basic concept of a proper and reliable propagation method of bear’s garlic on commercial scale.

Ograniczanie wyników

1 Herba Polonica

1 Figas A.

1 Keutgen

1 Keutgen N.

1 Tomaszewska-Sowa M.

1 2015

Wyniki wyszukiwania

Establishing an efficient explant superficial sterilization protocol for in vitro micropropagation of bear’s garlic (Allium ursinum L.)