Wyniki wyszukiwania

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Sex-sorting of boar spermatozoa does not influence the localization of glucose transporters

100%

Bucci D., Galeati G., Giaretta E., Tamanini C., Spinaci M.

Reproductive Biology

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2013

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tom 13

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nr 4

2

Embelin supplementation of in vitro maturation medium does not influence nuclear and cytoplasmic maturation of pig oocytes

86%

Galeati G., Giaretta E., Zannoni A., Bucci D., Tamanini C., Forni M., Spinaci M.

Journal of Physiology and Pharmacology

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2016

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tom 67

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nr 4

3

Angiogenic activity of porcine granulosa cells co-cultured with endothelial cells in a microcarrier-based three-dimensional fibrin gel

86%

Grasselli F., Basini G., Tirelli M., Cavalli V., Bussolati S., Tamanini C.

Journal of Physiology and Pharmacology

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2003

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tom 54

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nr 3

To verify the possible role played by pig granulosa cells in the ovarian angiogenic process, we have developed a reliable in vitro system which allows the evaluation of endothelial sprouting and capillary growth in three-dimensional matrices. Granulosa cells collected from porcine follicles of different size were co-cultured with porcine aortic endothelial cells (PAEC) in a microcarrier-based fibrin gel system; after 2 and 5 days of co-culture, we determined the number and length of all endothelial sprouts; moreover, these parameters were quantified only in capillary-like structures, which were defined as continuous multicellular sprouts at least 200 µm long. In granulosa cells- PAEC co-cultures we observed an increase of angiogenic activity as compared to controls (PAEC alone). Granulosa cells from follicles of different size regulate angiogenesis differently: cells from the small follicle group significantly enhanced endothelial sprouting, while those from the large follicle group favoured mainly capillary elongation. Our observations seem therefore to suggest that the development and growth of thecal vascular bed is controlled by paracrine factors of granulosa cell origin that may induce the formation of a primitive capillary plexus during the early phases of antral follicle growth, which will be remodelled in more advanced phases of follicular development.

4

Leptin does not seem to influence glucose uptake by bovine mammary explants

72%

Accorsi P. A., Gamberoni M., Isani G., Govoni N., Viggiani R., Monari M., De Ambrogi M., Munno A., Tamanini C., Seren E.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 4

Leptin, a protein produced and secreted by adipocytes, is know to regulate food intake and whole-body energy metabolism, but knowledge about its possible effect in bovine mammary gland is scarce. Leptin may be involved in the regulation of glucose transport even though this effect at the tissue level remains controversial. Once uptaken by the mammary gland, glucose is utilised in several ways but the majority, about 60-70%, is drained for lactose synthesis. This study was aimed at investigating the effect of leptin on glucose regulation in bovine mammary gland. We have examined the effects of leptin on the expression of GLUT1 mRNA, pyruvate kinase (PK) as well as glucose-6-phosphate dehydrogenase (G6PDH) activity. Treatment of mammary gland explants with recombinant leptin did not influence glucose assimilation, pathway transport (GLUT1 mRNA) and glucose metabolism (PK and G6PDH) in this tissue. The results from this study seem to exclude an involvement of leptin in glucose uptake and metabolism in bovine mammary gland.

Ograniczanie wyników

3 Journal of Physiology and Pharmacology

1 Reproductive Biology

4 Tamanini C.

2 Bucci D.

2 Galeati G.

2 Giaretta E.

2 Spinaci M.

1 Accorsi P. A.

1 Basini G.

1 Bussolati S.

1 Cavalli V.

1 De Ambrogi M.

1 Forni M.

1 Gamberoni M.

1 Govoni N.

1 Grasselli F.

1 Isani G.

1 Monari M.

1 Munno A.

1 Seren E.

1 Tirelli M.

1 Viggiani R.

1 Zannoni A.

1 2016

1 2013

1 2005

1 2003

Sex-sorting of boar spermatozoa does not influence the localization of glucose transporters

Embelin supplementation of in vitro maturation medium does not influence nuclear and cytoplasmic maturation of pig oocytes

Angiogenic activity of porcine granulosa cells co-cultured with endothelial cells in a microcarrier-based three-dimensional fibrin gel

Leptin does not seem to influence glucose uptake by bovine mammary explants