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Polymorphism of internal transcribed spacer 2 [ITS-2] sequences and genetic relationships between Fasciola hepatica and F.gigantica

100%

Semyenova S. K., Morozova E. V., Vasilyev V. A., Gorokhov V. V., Moskvin A. S., Movsessyan S. O., Ryskov A. P.

Acta Parasitologica

2005

tom 50

nr 3

Nucleotide sequences of internal transcribed spacer 2 (ITS-2) of nuclear DNA were obtained from 58 adult worms Fasciola hepatica and F. gigantica of naturally infected cattle and sheep in Russia, Ukraine, Belarus, Armenia, Uzbekistan, Turkmenistan and Tajikistan. No variation was observed between 43 liver flukes F. hepatica from Russia, Belarus, Ukraine, Armenia and Turkmenistan. Only one specimen from Armenia had a single unique transversion C-G (0.3% variation). F. gigantica from Turkmenistan, Tajikistan and Uzbekistan differed at four nucleotide transitions (1.1% variations). For comparative purpose, the ITS-2 sequences of two species from Europe, Africa, Asia, America, Australia and Oceania were used and evolution history of ITS-2 sequences of Fasciola species was reconstructed with statistical parsimony network (SPN) method. The relationships between F. hepatica and F. gigantica from different regions were discussed.

RAPD variability and genetic diversity in two populations of liver fluke, Fasciola hepatica

100%

Semyenova S. K., Morozova E. V., Chrisanfova G. G., Asatrian A. M., Movsessian S. O., Ryskov A. P.

Acta Parasitologica

2003

tom 48

nr 2

Random amplified polymorphic DNA (RAPD) markers were used to quantify the genetic diversity and its distribution in liver fluke, Fasciola hepatica recovered from seven definitive hosts belonging to Ukrainian and Armenian cattle populations. Five oligonucleotide primers produced complex and highly variable patterns of amplified DNA in F. hepatica. Intra- and interpopulation genetic variability of adult parasites was analyzed on the base of polymorphic and monomorphic reproducible bands. Genotypic diversity calculated by Shannon’s indices showed that the majority of variance occurred within, rather then between hosts and was also greater within than between populations. Analysis of molecular variance was used to test the significance of genetic differentiation within and between populations of worms. Of total gene diversity, 86.54% were partitioned within hosts, 8.17% of variability accounted by the differences among hosts/within populations and 5.3% of variation by the differences between populations. These results suggest that each individual cow is infected by numerous genetically different parasites. Migration of definitive hosts and other factors influencing the distribution of liver fluke RAPD variability are discussed.

Ograniczanie wyników

2 Acta Parasitologica

2 Morozova E. V.

2 Ryskov A. P.

2 Semyenova S. K.

1 Asatrian A. M.

1 Chrisanfova G. G.

1 Gorokhov V. V.

1 Moskvin A. S.

1 Movsessian S. O.

1 Movsessyan S. O.

1 Vasilyev V. A.

1 2005

1 2003

Wyniki wyszukiwania

Polymorphism of internal transcribed spacer 2 [ITS-2] sequences and genetic relationships between Fasciola hepatica and F.gigantica

RAPD variability and genetic diversity in two populations of liver fluke, Fasciola hepatica