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The yield and cell viability of bovine in vivo recovered embryos in relation to season of flushing

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Stadnik L., Makarevich A. V., Dolezalova M., Kubovicova E., Beran J., Hegedusova Z., Citek J., Holasek R., Stupka R.

Journal of Animal and Feed Sciences

2014

tom 23

nr 4

The objective of this study was to evaluate effect of the season on the yield and viability of embryos recovered from superovulated and flushed Holstein cows (n = 56). The embryo recovery rate (ERR, %) was determined as the proportion of totally flushed embryos (TFE, n) to the number of corpora lutea detected. The embryos were evaluated as transferable (TRANS) or non-transferable and analysed for occurrence of apoptotic cells (T+, TUNELindex) as well as actin cytoskeleton quality (ACG). TFE and ERR were the highest during spring vs summer (149 embryos, 59.6% vs 20 embryos, 37%; P < 0.001), while they insignificantly differed in autumn (57 embryos, 48.3%). The autumn embryos presented a higher proportion of TRANS (P < 0.05, +10.56% vs spring; +18.94% vs summer). The occurrence of apoptosis in these embryos was lower (–1.38 T+ cells and –1.75% T-index; P < 0.05) and the percentage of the best ACG was higher (+35%; P < 0.05). These findings identified autumn as the best season for collection of high-quality bovine embryos.

Ograniczanie wyników

1 Journal of Animal and Feed Sciences

1 Beran J.

1 Citek J.

1 Dolezalova M.

1 Hegedusova Z.

1 Holasek R.

1 Kubovicova E.

1 Makarevich A. V.

1 Stadnik L.

1 Stupka R.

1 2014

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The yield and cell viability of bovine in vivo recovered embryos in relation to season of flushing