The aim of this study was to evaluate the content of lipids and their effect on progesterone concentration in the blood serum of cows. Experimental material was sampled from lactating and dry cows (41 specimen) of Polish Holstein-Friesian breed Black and White sub-species cattle, aged 3 to 6 years (lactation month 4-6 and pregnancy month 9, respectively) in two different maintenance periods, i.e. in summer and winter. In blood samples for analyses, triglycerides (TG), phospholipids (PL), total cholesterol (Cht) and its HDL fraction were determined using a Biomerieux reagent kits and a Kontron 92 UV-VIS spectrophotometer. LDL fraction of total cholesterol was calculated from the following equation: LDL [mmol/L] = Cht - HDL - TG/2.2. Progesterone concentration was determined by radioimmunoassay. The results were subject to statistical analysis by means of a Statistica 6.0 computer software package. Analysis of variance (Anova) was performed at significance from the external jugular vein in the morning hours. The blood serum concentrations at a level of p £ 0.05 were carried out. Pearson’s coefficient of correlation (r) was calculated to determine relationship between the examined lipids and progesterone. A significantly higher triglyceride concentration (0.32 mmol/L) was found in the blood serum of dry cows when compared to the lactating ones. The lactating cows had a higher content of phospholipids in both examined maintenance seasons. The content of total cholesterol and its HDL and LDL fractions was higher in both examined groups in summer. Blood serum progesterone concentration in both seasons was higher in the pregnant cows. Most of the positive dependencies between the concentration of studied lipids and progesterone concentration were found, especially in dry cows in winter.
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