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Artemisia species in vitro cultures for production of biologically active secondary metabolites

100%

Grech-Baran M., Pietrosiuk A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2012

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tom 93

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nr 4

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Approaches of Rhodiola kirilowii and Rhodiola rosea field cultivation in Poland and their potential health benefits

81%

Grech-Baran M., Syklowska-Baranek K., Pietrosiuk A.

Annals of Agricultural and Environmental Medicine

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2015

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tom 22

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nr 2

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Tyrosol Glucosyltransferase Activity and Salidroside Production in Natural and Transformed Root Cultures of Rhodiola Kirilowii (Regel) Regel et Maximowicz

71%

Grech-Baran M., Syklowska-Baranek K., Giebultowicz J., Wroczynski P., Pietrosiuk A.

Acta Biologica Cracoviensia. Series Botanica

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2013

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tom 55

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nr 2

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Paclitaxel production and PAL activity in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene elicited with nitric oxide and methyl jasmonate

71%

Sykolowska-Baranek K., Grech-Baran M., Naliwajski M. R., Bonfill M., Pietrosiuk A.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 10

The role of sodium nitroprusside (SNP, 10 lM), a nitric oxide (NO) donor, and/or methyl jasmonate (MJ, 100 lM) spiked with L-phenylalanine (PHEN, 100 lM) and additional sucrose (S; 30 g l-1), in taxane production and phenyl ammonia lyase (PAL) activity in cultures of two Taxus media x var. Hicksii transgenic root lines (ATMA and ATM) carrying the taxadiene synthase transgene was investigated. SNP addition, when applied together with MJ and/or PHEN, resulted in paclitaxel production only in ATMA cultures. The application of the NO donor gave the highest paclitaxel content (7.56 mg l-1) in the combination of SNP+S+MJ+PHEN, after 2 weeks of treatment in the ATMA root line. In ATM cultures, taxane production was not affected by SNP. In both ATMA and ATM lines the highest total (intra+extracellular) paclitaxel yield was determined when elicited with MJ+PHEN, and amounted to 10.78 mg l-1 at 1 week and 1.63 mg l-1 at 2 weeks of treatment, in cultures of ATMA and ATM lines, respectively. The excretion of paclitaxel was observed only in ATMA cultures, with the highest level (2.34 mg l-1) obtained after elicitation with S+MJ+PHEN. The comparison of PAL activity in the two root lines revealed that this enzyme was almost 3-times more active in ATM than ATMA roots. An increase in both PAL activity and paclitaxel production was only observed in ATMA cultures growing in medium supplemented with S+MJ+PHEN.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Physiologiae Plantarum

1 Annals of Agricultural and Environmental Medicine

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

4 Grech-Baran M.

4 Pietrosiuk A.

2 Syklowska-Baranek K.

1 Bonfill M.

1 Giebultowicz J.

1 Naliwajski M. R.

1 Sykolowska-Baranek K.

1 Wroczynski P.

2 2015

1 2013

1 2012

Artemisia species in vitro cultures for production of biologically active secondary metabolites

Approaches of Rhodiola kirilowii and Rhodiola rosea field cultivation in Poland and their potential health benefits

Tyrosol Glucosyltransferase Activity and Salidroside Production in Natural and Transformed Root Cultures of Rhodiola Kirilowii (Regel) Regel et Maximowicz

Paclitaxel production and PAL activity in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene elicited with nitric oxide and methyl jasmonate