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1

Specyfika działania systemu antyoksydacyjnego nasienia knura

100%
Orzolek A., Mietelska K., Wysocki P.
Medycyna Weterynaryjna
|
2016
|
tom 72
|
nr 05
An excessive generation of reactive oxygen species (ROS) in boar semen leads to a reduced motility and fertilizing ability of spermatozoa. Boar spermatozoa, because of a high content of polyunsaturated fatty acids (PUFAs) in their structure, are highly sensitive to lipid peroxidation (LPO). This process, induced by ROS generation, causes irreversible changes in the conformation and integrity of plasmalemma. The boar’s reproductive system includes a special antioxidant system consisting of enzymatic components and antioxidants of low molecular weight. The most active of antioxidant enzymes present in boar semen is superoxide dismutase (SOD). SOD transforms superoxide anion (O₂˙⁻) into hydrogen peroxide (H₂O₂). Because H₂O₂ can easily diffuse across the membranes, it is most harmful to boar spermatozoa. Given the low content of antioxidants of low molecular weight (e.g. L-gluthatione or L-ergothioneine) and the absence of catalase (CAT) activity in boar semen, there must be other mechanisms responsible for the scavenging of hydrogen peroxide and other ROS. This function is probably accomplished mainly by phospholipid hydroperoxide gluthatione peroxidase (PHGPx), enzymes of tioredoxin (TRX) and peroxiredoxin (PRDX) groups, as well as by paraoxonase type 1 and 2 (PON-1 and PON-2).
2

Superoxide dismutase (SOD) in boar spermatozoa: Purification, biochemical properties and changes in activity during semen storage (16°C) in different extenders

100%
Orzolek A., Wysocki P., Strzezek J., Kordan W.
Reproductive Biology
|
2013
|
tom 13
|
nr 1
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Relation between nitric oxide (NO) level in semen and certain properties of boar spermatozoa stored at 17°C

88%
Orzolek A., Zasiadczyk L., Wysocki P., Kordan W., Krysztofiak P.
Polish Journal of Veterinary Sciences
|
2018
|
tom 21
|
nr 2
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Isolation and identification of zinc ions and heparin binding proteins in yellow fraction of the red deer Cervus Elaphus L. semen

76%
Orzolek A., Dyrda K., Rafalska K., Wysocki P., Kordan W., Gizejewski Z., Pluciennik M.
Polish Journal of Veterinary Sciences
|
2021
|
tom 24
|
nr 1
5

Boar sperm quality in relation to presence of sp32-like protein in spermatozoa - preliminary studies

76%
Orzolek A., Wysocki P., Strzezek J., Koziorowska-Gilun M., Dziekonska A., Kordan W.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 2
The aim of the study was to analyse sperm proteomes of ejaculates from Polish Large White (PLW) and Polish Landrace (PL) boars and to identify differences which putatively influence semen quality. Spermatozoa protein profiles were analysed by electrophoretic methods followed by selected techniques to evaluate semen quality on the following factors: sperm motility , lipid peroxidation levels (MDA production), ATP content, activities of superoxide dismutase (SOD) and catalase (CAT), total antioxidant status (TAS), and total oxidant status (TOS) of seminal plasma. A protein with an estimated molecular weight of 30 kDa was found in spermatozoa of selected ejaculates. Mass spectrometry demonstrated that this polypeptide is most similar to proacrosin binding protein (sp32). The presence of the protein was more frequently observed in sperm extracts obtained in spring-summer period. Ejaculates containing sp32-like protein demonstrated significantly higher spermatozoa motility, lower inhibition of MDA production by seminal plasma, and higher SOD activity in seminal plasma. Boar semen which included sp32-like protein also demonstrated lower ATP levels in spermatozoa as well as higher TAS and lower TOS of seminal plasma, though the differences were not statistically significant. Ejaculates from PLW boars, with sp32-like protein present in sperm, were characterised by significantly higher sperm motility , lower ATP content in spermatozoa, and higher TAS of seminal plasma. The diminished parameters of semen quality were observed in ejaculates from PL boars that also contained the discussed protein, but the differences were not statistically significant. These findings suggest that the presence of sp32-like protein in boar spermatozoa could influence semen quality.
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