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Partial purification and characterization of pectinmethylesterase from ripening guava (Psidium guajava L.) fruits

100%

Mondal K., Malhotra S. P., Jain V., Singh R.

Acta Physiologiae Plantarum

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2009

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tom 31

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nr 1

Employing the techniques of (NH₄)₂SO₄ fractionation, ion exchange chromatography on DEAE cellulose and gel filtration through Sephadex G-100, pectinmethylesterase (EC 3.1.1.11) was purified from guava (Psidium guajava L.) fruits var. Hisar Safeda harvested at turning stage of maturity to 129-fold with 28% recovery. Molecular weight as determined by gel filtration was found to be 51 kDa and the enzyme preparation exhibited the same molecular weight under native (Native-PAGE) and denaturating conditions (SDS-PAGE) indicating that the enzyme was a monomer. With pectin as the substrate, it exhibited the Michaelis Menten kinetics with Km value of 3.1 g l⁻¹. The enzyme was found to be stimulated by Ca⁺⁺ and Na⁺ and inhibited competitively by Dgalacturonic acid with Ki value of 1.97 mM. The enzyme was completely inactivated by iodine while with diethyl pyrocarbonate and N-acetylimidazole, the enzyme was inhibited up to the extent of 56 and 45%, respectively. However, DTNB had no inhibitory effect whatsoever precluding the participation of any –SH group in the active centre. It is tentatively proposed that the enzyme has tyrosine and histidine residues at its active centre.

2

Purification and characterization of trypsin inhibitor from seeds of faba bean [Vicia faba L.]

100%

Gupta P., Dhawan K., Malhotra S. P., Singh R.

Acta Physiologiae Plantarum

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2000

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tom 22

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nr 4

3

A simple method for in vivo labelling of lipid fractions in developing seeds of Brassica campestris L.

100%

Sharma N., Archana, Malhotra S. P., Singh R.

Acta Physiologiae Plantarum

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2003

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tom 25

|

nr 1

4

Compositional and enzymatic changes in guava [Psidium guajava L.] fruits during ripening

88%

Jain N., Dhawan K., Malhotra S. P., Siddiqui S., Singh R.

Acta Physiologiae Plantarum

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2001

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tom 23

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nr 3

Ograniczanie wyników

4 Acta Physiologiae Plantarum

4 Malhotra S. P.

4 Singh R.

2 Dhawan K.

1 Archana

1 Gupta P.

1 Jain N.

1 Jain V.

1 Mondal K.

1 Sharma N.

1 Siddiqui S.

1 2009

1 2003

1 2001

1 2000

Partial purification and characterization of pectinmethylesterase from ripening guava (Psidium guajava L.) fruits

Purification and characterization of trypsin inhibitor from seeds of faba bean [Vicia faba L.]

A simple method for in vivo labelling of lipid fractions in developing seeds of Brassica campestris L.

Compositional and enzymatic changes in guava [Psidium guajava L.] fruits during ripening