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The purpose of this study was to determine the effect of long-term exercise of varying intensity on selected hematological blood parameters of horses. The study was conducted on 24 clinically healthy half-blood horses. Blood for designations was collected twice: at the beginning of March, when the indoor season was approaching an end (a smaller number of starts), and at the end of May, when efforts were particularly intensified due to the continuous training and the beginning of the open season (more starts). The whole blood was examined for: the total number of erythrocytes (RBC), hematocrit (Ht), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and the red blood cell distribution width (RDW). The study was performed using an MS9 hematology analyzer. Mean values were compared between the retrievals (stallions and mares), and animals of both sexes were compared among themselves in collections I and II. The study showed that the growing physical effort of sport horses caused increased rates of hematological parameters, such as RBC, Ht, MCV, RDW in both stallions and mares in collection II compared to collection I. Studies of hematological parameters are an important element of planning and monitoring the workouts of horses.
The aim of the study was to assess the impact of varying intensity of exercise on ghrelin levels in half-blood jumping horses during training season. Blood samples were collected in the period of intense and moderate exercise. Serum concentrations of active and total ghrelin were measured using commercial RIA kits. Exercise did not cause the disruption in energy homeostasis, which was confirmed by the conducted tests. Total concentration of ghrelin measured during the increased effort intensity elevated in stallions (from 1031.45 to 1732.19 pg/mL at P ≤ 0.05). The opposite situation occurred in the case of active ghrelin, which concentration did not increase significantly in mares (from 26.21 to 28.46 pg/mL), whereas an insignificant decrease was noted in stallions (from 24.19 to 22.54 pg/mL). The only significant difference between stallions and mares was noted in the 2nd sampling (P = 0.015). The research proved that the fluctuations of both studied parameters did not reflect a negative impact of the increased effort.
Blood samples were collected from horses in four time points of the spring-winter training season, according to the difference in effort intensity characteristic for each period. Serum contents of glucose, cholesterol, and creatinine, as well as activity of creatine kinase, aspartate and alanine aminotransferases, and alkaline phosphatase were determined. Training did not cause muscle damage, and as a result of training, changes in the characteristics of both, contraction and biochemical properties of muscle tissue took place, which was confirmed by the conducted tests.
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