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Molecular marker assisted selection for Phytophthora capsici Leon. resistance lines in pepper (Capsicum annuum L.).

100%

Colak A. A., Yilmaz N.

Acta Scientiarum Polonorum. Hortorum Cultus

2020

tom 19

nr 3

The most important diseases that cause loss of productivity in all areas of pepper production in the world and the limitation of cultivation are Phytophthora crown blight of pepper (Phytophthora capsici Leon.). The disease is a soil borne pathogen, and its challenge is difficult. Selection of varieties resistant to diseases of soil-borne plant pathogens is the most efficient, economical and sustainable method. In this study, the resistance of Phytophthora crown blight (Phytophthora capsici Leon.) disease on 95 pepper genotype in terms of yield and some quality characteristics was investigated by using SCAR marker (OpD04.717). In the study, pepper lines in 7 pepper genotype F2 population showed durability band at 717 bp of agarose gel, while 88 pepper genotype were found to be sensitive. As a result of molecular studies, 7 pepper genotype resistant to Phytophthora capsici Leon. were also confirmed by classical test using seedling immersion method. In the study, as a result of molecular and classical tests, Phytophthora capsici Leon. is determined to be resistant to pepper lines in the hybridization programs to obtain new varieties and will be used as a parent or father will contribute to improvement studies.

Molecular characterization of Tomato yellow leaf curl virus and Fusarium oxysporum formae speciales and races of tomato areas in Northern Cyprus

80%

Colak A. A., Fdan H., Gokhan B.

Acta Scientiarum Polonorum. Hortorum Cultus

2020

tom 19

nr 3

Fusarium oxysporum (FO) and viruses have caused wilt, root, crown rots, mosaic, yellowing and curling on tomato plants and have resulted economic yield losses on tomato production areas at Northern Cyprus (NC) in 2011–2015 years. Typical FO symptoms showing greenhouse and open field areas used for collecting 62 plants and suspected Tomato yellow leaf curl virus (TYLCV) of 76 tomato plants have been studied respectively. In the researches, four different primers [uni,sp13,sp23,sprl] were used to determine the formae specialis and races of 62 isolates of FO isolates from different locations. PCR analyse studies have revealed that 81% of collected samples were Fusarium oxysporum f.sp. lycopersici (FOL) and 19% of them were Fusarium oxysporum f.sp. radicis-lycopersici (FORL). Additionally, further PCR analyses have identified that 37% of FOL samples were race 1, 15% were race 2 and 29% were race 3 identified respectively. The different samples of 76 tomato plants were tested with specific primers in PCR amplifications. Their results determined that the strains TYLCV-Israel, TYLCV-Sicilia, TYLCV-Mild of TYLCV found. The molecular techniques have suggested that Israel, Sicilia and Mild strain of TYLCV were present in the tomato production areas at NC. The TYLCV races with single or mixed infections and Fusarium oxysporum formae specialis and races are able to identify in molecular techniques in not only accurately but also reliably.

Ograniczanie wyników

2 Acta Scientiarum Polonorum. Hortorum Cultus

2 Colak A. A.

1 Fdan H.

1 Gokhan B.

1 Yilmaz N.

2 2020

Wyniki wyszukiwania

Molecular marker assisted selection for Phytophthora capsici Leon. resistance lines in pepper (Capsicum annuum L.).

Molecular characterization of Tomato yellow leaf curl virus and Fusarium oxysporum formae speciales and races of tomato areas in Northern Cyprus