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Optimal expression and purification of sapelovirus A structural protein VP1, and its immunogenicity in mice

100%

Zhao T. T., Cui L., Chen L., Li J. J., Liang Q. L., Wu P. J., Yu X. Q., Zhang Z. H., Hua X. G.

Polish Journal of Veterinary Sciences

2018

tom 21

nr 3

Vertical transmission of PCV2b to fetuses in sows intramuscularly infected with PCV2b

100%

Wang N. D., Li J. J., Wang A. B., Zhu Z., Chang Y., Mao Y., Zan Y., Wang Z. F., Deng Z. B.

Polish Journal of Veterinary Sciences

2016

tom 19

nr 3

In order to investigate whether PCV2b can be transmitted across the placenta in sows thereby causing vertical infection of fetuses, six sows in 75 day of pregnancy were either intramuscularly inoculated with a PCV2b culture supernatant (n = 4) or mock infected with cell culture supernatant (n = 2). At parturition, 3 newborn piglets from each litter were randomly selected and euthanatized prior to suckling. Samples of the mesenteric lymph nodes, spleens, kidneys, hearts and lungs were collected for PCR, histopathological and immunohistochemical assays. The results showed that the newborn piglets from PCV2b-infected sows had histopathological lesions in the tested tissues. Moreover, PCV2b antigen and DNA were detected as well. These findings therefore suggested that porcine circovirus type 2b can be transmitted across the placenta of sows, thereby leading to PCV2b vertical infection of the fetuses.

Ograniczanie wyników

2 Polish Journal of Veterinary Sciences

2 Li J. J.

1 Chang Y.

1 Chen L.

1 Cui L.

1 Deng Z. B.

1 Hua X. G.

1 Liang Q. L.

1 Mao Y.

1 Wang A. B.

1 Wang N. D.

1 Wang Z. F.

1 Wu P. J.

1 Yu X. Q.

1 Zan Y.

1 Zhang Z. H.

1 Zhao T. T.

1 Zhu Z.

1 2018

1 2016

Wyniki wyszukiwania

Optimal expression and purification of sapelovirus A structural protein VP1, and its immunogenicity in mice

Vertical transmission of PCV2b to fetuses in sows intramuscularly infected with PCV2b