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The aim of this paper was to measure the influence of different forms of nitrogen (NO₃⁻, NO₂⁻, NH₄⁺) on N₂O consumption and dehydrogenase activity these being the index of soil oxidative activity in the soils developed from sand and loam under flooded conditions. Both soils were able to produce and consume nitrous oxide. The processes of N₂O production and consumption depended on N-amendment. Loamy soil amended with nitrate(V) produced additionally 65.7 mg kg⁻¹ N₂O-N during seven days of incubation and then N₂O consumption (produced and added - 142.4 mg kg⁻¹ N₂O-N) was observed. In soil developed from sand amended with nitrate(V) production of N₂O was equal to 19.1 mg kg⁻¹ N₂O-N during first three days of incubation and small consumption (7.8 mg kg⁻¹ N₂O-N) was observed after that period. NH₄⁺ addition did not influence N₂O consumption in both investigated soils. NO₃⁻ and NH₄⁺ addition inhibited dehydrogenase activity in the case of the two investigated soils compared with the control. There was observed some ‘transition’ stage clearly visible between phases of adaptation to utilization of nitrous oxide as electron acceptors both in the case where N₂O was added and was produced in the denitrification process. Nitrous oxide consumption probably occurred by another biochemical pathway than reduction to N₂ (denitrification) reflected in the change of dehydrogenase activity. NO₂⁻ amendment inhibited all investigated biochemical activities. Soil ability to N₂O consumption was positively correlated with the dehydrogenase activity.
The actual denitrification to N 2 O and denitri- fication capacity to N 2 O after flooding of different soil samples stored for over 25 years in air-dry conditions and fresh, air dried samples were compared in our study. Zero N 2 O release was ob- served from the stored soils but the fresh soil samples had very low actual denitrification to N 2 O. NO 3 - addition significantly increased the amount of N 2 O (denitrification capacity to N 2 O) released after flooding, which depended on the length of storage and type of soils and was much higher in stored soils. Prolonged exposure of the soils to drought conditions caused a greater decrease in the Eh value compared with the fresh soil. The total cumulative release of N 2 O from the stored and fresh soils was correlated with the reduced NO 3 - and organic C content in soils enriched with NO 3 - . Some soils showed the capability of N 2 O consumption. CO 2 release depended on the length of storage and type of soils under flooding after pro- longed drought. On average, CO 2 release was higher from the stored rather than fresh soils. The organic C content in the stored soils was generally lower than in the fresh soils, probably due to the storage effect. The cumulative CO 2 release from the stored soils was well correlated with the organic C while no correlation was observed for the fresh soil samples.
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