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Changes in the amino acid composition during the ensiling of lucerne and red clover in round bales

100%
Purwin C., Fijalkowska M., Lipinski K., Wierzbowska J., Kobzhassarov T. Z., Michalski J.
Journal of Elementology
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2015
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tom 20
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nr 4
Changes in the amino acid profile of protein during ensiling depend on the proteolytic potential of plant species, the rate of the wilting and acidifying of ensiled herbage and, on the other hand, on the microbiological processes and fermentation of amino acids. The interaction of these factors determines the subsequent efficiency of nitrogen utilization in the rumen. The aim of this study has been to determine the influence of ensiling in round bales on changes in the amino acid content in lucerne and red clover silages. The amino acid composition was determined by analyzing the content of amino acids in herbages and silages. The lucerne silage protein was characterized by a higher contribution of all amino acids except Trp in comparison with red clover silage. Ensiling in bales of wilted lucerne and clover herbage lowered the content of Asp, His (P < 0.01), Ile, Lys (P < 0.05) in both plants. The ensiling process and plant species affected (P < 0.05) the total content of amino acids per 100 g of protein, where the range of changes in both raw materials was different. The loss of amino acids observed during the ensiling of wilted lucerne in round bales should be regarded as typical, whereas changes in the amino acid profile of red clover should be considered as atypical. A significantly higher ADIN content in red clover than in lucerne silage indicates that a rapid and significant increase of temperature occurred in experimental bales, which were characterized by a low degree of density, and this temperature rise strongly affected the availability of amino acids of the red clover protein also to the current microflora in the silage. Ensiling in round bales changed the amino acid profile of the lucerne and red clover protein. The ensiling process in this technology, however, severely deteriorated the quality of the red clover protein by lowering the content of all essential amino acids except Cys and Met.
2
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Influence of a diet containing beta-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of experimental calf rearing

84%
Zwierzchowski G., Micinski J., Pogorzelska J., Siwicki A., Wojcik R., Kobzhassarov T. Z., Bermagambetova N., Shaikamal G. I., Fijalkowska M.
Journal of Elementology
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2016
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tom 21
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nr 1
The aim of this study has been to determine the effect of a diet containing β-carotene and omega-3 fatty acids on the biochemical and nonspecific humoral immunity indicators and on the results of rearing calves to day 60 of life. The study was conducted in 2011-2012, on a herd of Polish Holstein-Friesian cows. 54 calves from the herd were divided into 3 groups, 18 animals in each: CTR, TRT1 and TRT2. During the colostral period lasting for 5 days after birth, calves were drinking the mother’s colostrum. From 6 to 60 days, calves were watered milk replacer formulation. The control group (CTR) consisted of calves after the colostral period given milk replacer formulation without any supplementation. In the two other groups, calves received milk replacers with the supplementation of b-carotene (TRT1 group) in the amount of 25 mg/calf/day or a suspension of liver omega-3 oil (TRT2 group) in the amount of 5g/calf/day. All calves received solid feed ad libitum. The research showed that the parameters of the metabolic profile of calves (ALT, ASP, CHOL, GLU, UREA, ALP, TG, CRE) were within the normal range, indicating good health of the calves. Moreover, the supplementation had a positive impact on the chosen immunological parameters, including a higher IgG concentration, especially at the end of the milk drinking period. The result of B-carotene in a diet was the highest daily gain in the period from 30 to 60 days of life among the analysed groups of calves. No such effect was achieved in the TRT2 group of calves, as their body weight was the lowest. However, noteworthy is the beneficial impact of beta-carotene and omega-3 oil on the non-specific humoral immunity parameters, which was manifested by fewer cases of clinical diarrhoea and upper respiratory tract diseases.
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