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1
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Content available

Boar variability affects sperm metabolism activity in liquid stored semen at 5 degrees Celsius

100%
Dziekonska A., Strzezek J.
Polish Journal of Veterinary Sciences
|
2011
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tom 14
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nr 1
Metabolic activity of boar spermatozoa, liquid stored for three days at 5℃, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5℃ may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.
2

Akceptacja konsumencka zywnosci modyfikowanej genetycznie

81%
Pyryt B., Kolenda H., Dziekonska A.
Bromatologia i Chemia Toksykologiczna
|
2008
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tom 41
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nr 3
234-237
3

Effect of ostrich egg lipoproteins and hen egg yolk on the quality of dog sperm during liquid storage at 5°C

81%
Dziekonska A., Behrendt D., Strzezek R., Kordan W.
Annals of Animal Science
|
2018
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tom 18
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nr 1
4

Effect of different storage temperatures on the metabolic activity of spermatozoa following liquid storage of boar semen

81%
Dziekonska A., Fraser L., Strzezek J.
Journal of Animal and Feed Sciences
|
2009
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tom 18
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nr 4
5

The ability of zinc-binding proteins from boar seminal plasma to bind heparin and phosphorylcholine

81%
Mogielnicka-Brzozowska M., Strzezek J., Dziekonska A., Kordan W.
Animal Science Papers and Reports
|
2014
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tom 32
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nr 4
Zinc-binding proteins (ZnBPs) isolated from boar seminal plasma exert multiple effects on boar spermatozoa. The aim of this study was to determine whether the isolated zinc-binding proteins were capable of binding additional ligands. The affinity chromatography method was used for the first time to demonstrate that boar ZnBPs show affinity for both heparin (hep) and phosphorylcholine (pch). ZnBPs+hep accounted for approximately 20%, and ZnBPs+pch – for approximately 45% of total ZnBPs. Predominant polypeptides with molecular mass form 6.5 to 14 kDa were observed in both groups (ZnBPs+hep, ZnBPs+pch). The analyzed peptides’ low molecular mass and their ability to bind zinc ions as well as heparin and phosphorylcholine suggest that they belong to the family of multifunctional boar spermadhesins which perform their functions in the fertilization process by binding more than one ligand. The results indicate that the ZnBPs of boar seminal plasma may be involved in the processes associated with oocyte fertilization not only by zinc ions binding but also through heparin and phosphorylcholine.
6

Metabolic activity of boar semen stored in different extenders supplemented with ostrich egg yolk lipoproteins

71%
Dziekonska A., Kinder M., Fraser L., Strzezek J., Kordan W.
Journal of Veterinary Research
|
2017
|
tom 61
|
nr 1
7
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Content available

Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa

71%
Kordan W., Lecewicz M., Strzezek R., Dziekonska A., Fraser L.
Polish Journal of Veterinary Sciences
|
2010
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tom 13
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nr 4
The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.
8

Effects of dietary supplementation with polyunsaturated fatty acids and antioxidants on biochemical characteristics of boar semen

71%
Strzezek J., Fraser L., Kuklinska M., Dziekonska A., Lecewicz M.
Reproductive Biology
|
2004
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tom 04
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nr 3
9
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Content available

Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa

61%
Dziekonska A., Swiader K., Koziorowska-Gilun M., Mietelska K., Zasiadczyk L., Kordan W.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 1
The aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17°C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p<0.05) were noted between F1 and F2 in progressive motility (PMOT), velocity average pathway (VAP) and velocity straight line (VCL). After the ejaculate was diluted, the type of fraction and type of extender significantly affected (p<0.05) PMOT, being markedly higher (p<0.05) for F1 extended in BTS. No marked changes (p<0.05) were observed between F1 and F2 extended in SCP for any of the analyzed sperm quality parameters during seven days of storage. Significantly higher (p<0.05) values of sperm quality parameters were noted in F1 compared with F2 for BTS on D7 of storage. The results of the four-way ANOVA analysis indicate that boar, fraction of ejaculate, extender type and day of storage had significant effects on the quality of boar stored spermatozoa. The F1 was characterised by higher quality of spermatozoa during storage in comparison with F2 in the short-term extender. Using the long-term extender containing the proteins allowed for a better application of F2, which could be important for the pig industry.
10
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Content available

Seasonal-dependent variations in metabolic status of spermatozoa and antioxidant enzyme activity in the reproductive tract fluids of wild boar/domestic pig hybrids

61%
Dziekonska A., Fraser L., Koziorowska-Gilun M., Strzezek J., Koziorowski M., Kordan W.
Polish Journal of Veterinary Sciences
|
2014
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tom 17
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nr 2
This study investigated seasonal changes in the metabolic performance of spermatozoa and activity of the antioxidant enzymes in the seminal plasma of three wild boar/domestic pigs (aged 1.5 to 2.5 years) and the activity of the antioxidant enzymes in fluids of the cauda epididymidis and vesicular glands from 16 wild boar/domestic pig hybrids (aged 1 to 3 years). Parameters of the sperm metabolic activity, such as total motility, mitochondrial functions, and measurements of oxygen uptake, ATP content and L-lactate production, were analyzed during the spring-summer and autumn-winter periods. Besides these sperm metabolic parameters, the sperm membrane integrity was also assessed. Total protein content and activity of the antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured in the reproductive tract fluids. There were no marked significant differences (P > 0.05) between the seasonal periods in terms of sperm motility, mitochondrial function and oxygen uptake; however, spermatozoa collected during the autumn-winter period exhibited higher (P < 0.05) ATP content and L-lactate production than those harvested during the spring-summer period. It was found that the vesicular gland fluid exhibited a higher level of SOD activity during the spring-summer period compared with the autumn-winter period. Furthermore, CAT activity in the seminal plasma and vesicular gland fluid was greater during the autumn-winter. Total protein content was significantly higher in the vesicular gland fluid, whereas the cauda epididymidal fluid exhibited greater SOD and GPx activities, irrespective of the seasonal period. The findings of this study confirmed seasonal-related differences in the metabolic performance of spermatozoa and activity of antioxidant enzymes of the reproductive tract of the boar/domestic pig hybrids.
11
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Content available

Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17degrees celcius

61%
Dziekonska A., Fraser L., Majewska A., Lecewicz M., Zasiadczyk L., Kordan W.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 3
This study was aimed to analyze the metabolic activity and membrane integrity of boar spermatozoa following storage in long-term semen extenders. Boar semen was diluted with Androhep® EnduraGuard™ (AeG), DILU-Cell (DC), SafeCell Plus™ (SCP) and Vitasem LD (VLD) extenders and stored for 10 days at 17°C. Parameters of the analyzed sperm metabolic activity included total motility (TMOT), progressive motility (PMOT), high mitochondrial membrane potential (MMP) and ATP content, whereas those of the membrane integrity included plasma membrane integrity (PMI) and normal apical ridge (NAR) acrosome. Extender type was a significant (P < 0.05) source of variation in all the analyzed sperm parameters, except for ATP content. Furthermore, the storage time had a significant effect (P < 0.05) on the sperm metabolic activity and membrane integrity during semen storage. In all extenders the metabolic activity and membrane integrity of the stored spermatozoa decreased continuously over time. Among the four analyzed extenders, AeG and SCP showed the best performance in terms of TMOT and PMI on Days 5, 7 and 10 of storage. Marked differences in the proportions of spermatozoa with high MMP were observed between the extenders, particularly on Day 10 of storage. There were not any marked differences in sperm ATP content between the extenders, regardless of the storage time. Furthermore, the percentage of spermatozoa with NAR acrosomes decreased during prolonged storage, being markedly lower in DC-diluted semen compared with semen diluted with either AeG or SCP extender. The results of this study indicated that components of the long-term extenders have different effects on the sperm functionality and prolonged semen longevity by delaying the processes associated with sperm ageing during liquid storage.
12

Boar sperm quality in relation to presence of sp32-like protein in spermatozoa - preliminary studies

61%
Orzolek A., Wysocki P., Strzezek J., Koziorowska-Gilun M., Dziekonska A., Kordan W.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 2
The aim of the study was to analyse sperm proteomes of ejaculates from Polish Large White (PLW) and Polish Landrace (PL) boars and to identify differences which putatively influence semen quality. Spermatozoa protein profiles were analysed by electrophoretic methods followed by selected techniques to evaluate semen quality on the following factors: sperm motility , lipid peroxidation levels (MDA production), ATP content, activities of superoxide dismutase (SOD) and catalase (CAT), total antioxidant status (TAS), and total oxidant status (TOS) of seminal plasma. A protein with an estimated molecular weight of 30 kDa was found in spermatozoa of selected ejaculates. Mass spectrometry demonstrated that this polypeptide is most similar to proacrosin binding protein (sp32). The presence of the protein was more frequently observed in sperm extracts obtained in spring-summer period. Ejaculates containing sp32-like protein demonstrated significantly higher spermatozoa motility, lower inhibition of MDA production by seminal plasma, and higher SOD activity in seminal plasma. Boar semen which included sp32-like protein also demonstrated lower ATP levels in spermatozoa as well as higher TAS and lower TOS of seminal plasma, though the differences were not statistically significant. Ejaculates from PLW boars, with sp32-like protein present in sperm, were characterised by significantly higher sperm motility , lower ATP content in spermatozoa, and higher TAS of seminal plasma. The diminished parameters of semen quality were observed in ejaculates from PL boars that also contained the discussed protein, but the differences were not statistically significant. These findings suggest that the presence of sp32-like protein in boar spermatozoa could influence semen quality.
13
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Content available

Effects of the platelet - activating factor (PAF) on selected quality parameters of cryopreserved bull semen (AI) with reduced sperm motility

61%
Lecewicz M., Kordan W., Majewska A., Kaminski S., Dziekonska A., Mietelska K.
Polish Journal of Veterinary Sciences
|
2016
|
tom 19
|
nr 1
The aim of the study was to determine the effects of platelet-activating factor (PAF) on selected quality parameters of cryopreserved bull semen with reduced sperm motility used for artificial insemination. The aim of experiment 1 was to identify the optimal concentration of the phospholipid able to preserve sperm viability. Cryopreserved semen was treated with different PAF concentrations: 1x10-5M, 1x10-6M, 1x10-7M, 1x10-8M and 1x10-9M. The experiment demonstrated that PAF at concentration 1x10-9M increased most the sperm viability parameters (motility parameters, plasma membrane integrity and mitochondrial function) after 120 min of incubation of thawed semen at 37oC. Cryopreserved bull semen with reduced sperm motility (below 70%) was supplemented with PAF in a concentration of 1x10-9M. A statistically significant increase in sperm motility, percentage of linear motile spermatozoa and VSL value was observed after 120 min incubation of sperm with 1x10-9M PAF. Sperm supplementation with PAF also had positive effects on plasma membrane integrity and percentage of spermatozoa with preserved mitochondrial transmembrane potential, but the differences were not statistically significant. The results indicated positive effects of PAF supplementation at a concentration of 1x10-9M on the selected sperm quality parameters in cryopreserved bull semen with reduced motility.
14

Effect of seminal plasma zinc–binding proteins on motility and membrane integrity of canine spermatozoa stored at 5°C

61%
Mogielnicka-Brzozowska M., Dziekonska A., Strzezek R., Zalecki M., Majewska A., Tolscik K., Kordan W.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 1
Sperm surface binding sites for non-zinc-binding proteins (nZnBPs) and zinc-binding proteins (ZnBPs) were studied by the fluorescence technique with biotin-labelled proteins. The nZnBPs binding pattern was unspecific, no characteristic sites on plasmalemma were found. ZnBPs were attached mainly to the acrosomal region of sperm head and to the sperm flagellum. ZnBPs added to the incubation mixture of the canine spermatozoa allowed the preservation of higher values for total motility, progressive motility, curvilinear line velocity, straight line velocity, and beat cross frequency (P < 0.05), both at time 0 and after 1 h incubation at 5°C. The addition of nZnBPs to the incubation mixture caused only weak positive effects when compared with control sample (PBS). A higher percentage of canine-ejaculated spermatozoa with intact membranes were observed when ejaculate was incubated with ZnBPs in comparison to control sample stored with PBS (P < 0.05) or nZnBPs (P < 0.05). Spermatozoa diluted with ZnBPs and nZnBPs exhibited a higher percentage of cells with active mitochondria when compared with control, both at time 0 and after 1 h; however, no statistical differences were observed. Our results emphasise the role of seminal plasma protein in securing the correct quantity and availability of zinc ions as a component regulating the motility of canine spermatozoa. The protective effect of ZnBPs against the cooling effect may be due to their ability of preventing sperm membrane damage.
15
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Content available

Semen quality assessments and their significance in reproductive technology

51%
Kordan W., Fraser L., Wysocki P., Strzezek R., Lecewicz M., Mogielnicka-Brzozowska M., Dziekonska A., Soliwoda D., Koziorowska-Gilun M.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 4
Semen quality assessment methods are very important in predicting the fertilizing ability of persevered spermatozoa and to improve animal reproductive technology. This review discusses some of the current laboratory methods used for semen quality assessments, with references to their relevance in the evaluation of male fertility and semen preservation technologies. Semen quality assessment methods include sperm motility evaluations, analyzed with the computer-assisted semen analysis (CASA) system, and plasma membrane integrity evaluations using fluorescent stains, such as Hoechst 33258 (H33258), SYBR-14, propidium iodide (PI), ethidium homodimer (EthD) and 6-carboxyfluorescein diacetate (CFDA), and biochemical tests, such as the measurement of malondialdehyde (MDA) level. This review addresses the significance of specific fluorochromes and ATP measurements for the evaluation of the sperm mitochondrial status. Laboratory methods used for the evaluation of chromatin status, DNA integrity, and apoptotic changes in spermatozoa have been discussed. Special emphasis has been focused on the application of proteomic techniques, such as two-dimensional (2-D) gel electrophoresis and liquid chromatography mass spectrometry (LC-MS/MS), for the identification of the properties and functions of seminal plasma proteins in order to define their role in the fertilization-related processes.
16
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Content available

Effects of storage in different semen extenders on the pre - freezing and post - thawing quality of boar spermatozoa

51%
Dziekonska A., Zasiadczyk L., Lecewicz M., Strzezek R., Koziorowska-Gilun M., Fraser R., Mogielnicka-Brzozowska M., Kordan W.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
|
nr 4
The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17°C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spermatozoa included: motility, normal apical ridge (NAR) acrosome, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), measurements of ATP content and activity of superoxidase dismutase (SOD) and glutathione peroxidase (GPx). Prior to the freezing process, no significant effect of the extender on the sperm quality parameters was noted. After thawing the spermatozoa it was demonstrated that the type of extender used influenced PMI, MMP, ATP content and activity of GPx. In the AH extender the percentage of spermatozoa with PMI and ATP content in spermatozoa was significantly higher (P<0.05) as compared to the BTS or GD extenders. In addition, semen stored in the AH was characterised by a statistically higher (P<0.05) percentage of spermatozoa with MMP and increased activity of GPx as compared with the BTS. The results obtained indicate that for the cryopreservation process, boar spermatozoa stored for 24 hours in liquid state can be used. However, the type of extender used prior to freezing may have a significant effect on the post- thawing quality of the spermatozoa. The AH extender better secured the quality of thawed boar spermatozoa as compared with the BTS or GD.
17
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Content available

Identification of proteoforms of albumin and kallikrein in stallion seminal plasma and their correlations with sperm motility

51%
Mogielnicka-Brzozowska M., Fraser L., Dziekonska A., Gackowska K., Sobiewska M., Kuzborska A., Majewska A. M., Filipowicz K., Kordan W.
Polish Journal of Veterinary Sciences
|
2019
|
tom 22
|
nr 2
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