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Optimizing culture for in vitro pollination and fertilization in cucumis sativus and C. melo

100%
Skalova D., Navratilova B., Ondrej V., Lebeda A.
Acta Biologica Cracoviensia. Series Botanica
|
2010
|
tom 52
|
nr 1
111-115
In vitro pollination can be used to overcome crossing barriers in interspecific hybridization within the genus Cucumis. This technique offers a way to produce viable interspecific hybrids. We tested two types of media, designated CP and YS, for in vitro pollination in Cucumis sativus and C. melo. Pollen grains were isolated by centrifugation or directly from mature male flowers and were cultured with mature ovules. We assessed pollen grain viability, fertilization ability, and fertilized ovule development. The developing ovules (becoming enlarged and green) were transferred to media supporting embryogenesis (with ascorbic acid, caseinhydrolysate, coconut water and gibberellic acid). The highest level of regeneration after in vitro pollination was callus formation from ovules. We found caseinhydrolysate to be the most beneficial component during in vitro pollination (CP medium) and during development of fertilized ovules (ON medium). The hybrid character of fertilized ovules arisen from crosses between cucumber and muskmelon was checked but not confirmed by RAPD analysis, for reasons we suggest. The in vitro protocol needs to be optimized further to obtain a high yield of potential hybrid embryos.
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Progress in research of embryo cultures in Cucumis spp.

100%
Skalova D., Navratilova B., Lebeda A.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
3

Interspecific hybridization between Cucumis sativus and wild Cucumis species through embryo culture

100%
Skalova D., Gasmanova N., Lebeda A., Navratilova B.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2005
|
tom 47
|
nr 1
4

Progress in research of cucurbitaceae protoplast cultures

100%
Gajdova J., Navratilova B., Lebeda A.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
5

Genome size variation of Lotus peregrinus at evolution canyon I microsite, Lower Nahal Oren, Mt. Carmel, Israel

76%
Gasmanova N., Lebeda A., Dolezalova I., Cohen T., Pavlicek T., Fahima T., Nevo E.
Acta Biologica Cracoviensia. Series Botanica
|
2007
|
tom 49
|
nr 1
39-46
On the basis of previous studies showing a positive correlation between number of copies of retrotransposons and geographical environment, we hypothesized that different ecogeographical conditions on opposite slopes of Evolution Canyon I could cause intraspecific variation in plant genome size. To test this hypothesis, we chose Lotus peregrinus L. (annual, self-pollinator) as the first candidate because of its biological contrast to the previously studied carob tree (long-lived, cross-pollinator). Absolute nuclear DNA content of 60 genotypes of L. peregrinus was estimated by PI flow cytometry, with tomato (Lycopersicon esculentum cv. Stupicke) as internal reference standard. The mean 2C-value in L. peregrinus was 2.546 pg, ranging from 2.39 pg to 2.71 pg. The mean 2C-value was higher in plants from the south-facing slope (2.549 pg) than from the north-facing slope (2.544 pg), but we were not able to show significant interslope differences in genome size.
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