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Molecular epidemiology of Salmonella enterica serovar enteritidis strains by pulsed-field gel electrophoresis isolated in the Slovak Republic

100%

Majtanova L., Szaboova M., Majtan V.

Polish Journal of Microbiology

2004

tom 53

nr 4

A total 44 isolates of S. enterica serovar Enteritidis (S. Enteritidis) belonged to three different phage types (PTs) 9a, 13a, 25 were analyzed by the technique of pulsed-field gel electrophoresis (PFGE). Thirty and three strains were from two outbreaks (central and southern regions of the Slovak Republic PTs 13a, 25) and 11 isolates were sporadic isolates (PT9a). These isolates showed two different patterns in PFGE with Xbal digestion. Strains of PT13a generated PFP A and isolates of PT25 showed uniform PFP B. Nine sporadic isolates of PT9a belonged to PFP A and two isolates to PFP B. The PFPs A and B were differed by only two bands. The distribution of Xbal profiles did not corresponded with PTs. We conclude that the close genetic similarity observed between epidemiologically unrelated and outbreak-related isolates ofS. Enteritidis suggest clonal relationship of these isolates.

Molecular characterization of class 1 integrons in clinical strains of Salmonella Typhimurium isolated in Slovakia

100%

Majtanova L., Majtan T., Majtan V.

Polish Journal of Microbiology

2007

tom 56

nr 1

The presence of class 1 integrons was investigated in 156 epidemiologically unrelated Salmonella Typhimurium isolates. Of these 156 isolates, 70 were of definitive phage type DT104 and 86 were strains of various phage type, RDNC and untypable, designated here as non-DT104 strains. Intégrons were found in 47 of DT104 isolates (67.1%), while in all strains with characteristic pentaresistance (R-type ACSSuT) two intégrons 1.0 kb and 1.2 kb in size were found. Among 86 non-DT 104 strains, intégrons with sizes of 1.6 kb and 1.9 kb in four multidrug-resistant strains DT193 and U302 were found. The intégrons from selected strains were further sequenced and the aadA1, aadA2, dlifrl, dhfr12 and blaPSE genes were found embedded in cassettes.

Ograniczanie wyników

2 Polish Journal of Microbiology

2 Majtan V.

2 Majtanova L.

1 Majtan T.

1 Szaboova M.

1 2007

1 2004

Wyniki wyszukiwania

Molecular epidemiology of Salmonella enterica serovar enteritidis strains by pulsed-field gel electrophoresis isolated in the Slovak Republic

Molecular characterization of class 1 integrons in clinical strains of Salmonella Typhimurium isolated in Slovakia