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Identifying the grain chalkiness gene using molecular marker techniques in rice (Oryza sativa L.)

100%

Thi Lang N., Ho Truc Giang P., Thi Thu Ha P., Bao Toan T., Anh Phuong T., Chi Buu B.

International Letters of Natural Sciences

2017

tom 63

Chalkiness is a major constraint on rice production because it is one of the key factors determining grain quality (appearance, processing, milling, storing, eating, and cooking quality) and price. In this study, we conducted grain chalkiness gene identification using co-dominant insertion/deletion (INDEL) markers and SSR marker combination on 50 different varieties. The application results in 7 InDel markers and SSR marker on chromosome 7 were recorded. Three primers, InDel 5, InDel 14 and RM21938, associated with grain chalkiness. For the InDel 5 primer, the amplification product was 100%. Use of primer InDel 5 in detection and evaluation of genotype to the chalkiness trait of rice grain on 50 rice varieties indicated the suitability level with phenotypic evaluation was 86% and the unsuitability level was 14%. For the InDel 14 primer, the amplification products were 100%. The suitability with phenotypic assessment was 84% and the unsuitability was 16%. For the RM21938 primer, the amplification product was 94%. The suitability with phenotypic assessment was 76% and the unsuitability was 24%. Thirteen of the selected varieties had grain chalkiness gene both InDel 5, InDel 14 and RM21938. Total 13 varieties were detected from InDel 5, InDel 14 and RM12938 primer combinations also showed high efficiency of the InDel technique in identifying chalkiness gene in rice grain. A cluster analysis was performed and a dendrogram was constructed which evinced the nature of phylogenetic classification among the genotypes of the varieties. These markers could be used for developing quality of rice in breeding program.

Correlation among agro-morphological variation and genetic diversity of rice (Oryza sativa L.) under drought stress

84%

Thu Ha P. T., Khang D. T., Tuyen P. T., Minh L. T., Minh T. N., Thi Lang N., Buu B. C., Xuan T. D.

International Letters of Natural Sciences

2016

tom 58

In this study, the correlation coefficients among agro-morphological variation, genetic diversity, and drought tolerance in 44 rice cultivars were analyzed. The drought tolerance at seeding stage (DTS) was significantly proportional to drought tolerance at vegetative stage (DTV) (r = 0.60). In addition, DTS and DTV had strong significant positive correlation to leaf roll (r = 0.87 and 0.54, respectively). Means of unfilled grains and tilling per panicle were proportionally correlated to DTS (r = 0.22 and 0.25, respectively), and DTV (r = 0.20 and 0.36, respectively). However, weight of 1000 grains and filled grains were recorded no correlation to DTS and DTV. At a homologous coefficient of 16.85 integrated from cluster analysis of agro-morphological, quantitative characteristics and drought tolerant scores, the rice cultivars were divided into five groups. Maximum polymorphic information content (PIC) values were detected in three markers including RM11125, RM21, and RM5629, which were from 0.78 to 0.79. Cluster analysis of microsatellite markers revealed that by a genetic distance of 0.63, the rice varieties were separated into three clusters. The results provide valuable information for rice breeders to select donors in breeding rice integrated with drought tolerance and good agronomic characteristics.

Ograniczanie wyników

2 International Letters of Natural Sciences

2 Thi Lang N.

1 Anh Phuong T.

1 Bao Toan T.

1 Buu B. C.

1 Chi Buu B.

1 Ho Truc Giang P.

1 Khang D. T.

1 Minh L. T.

1 Minh T. N.

1 Thi Thu Ha P.

1 Thu Ha P. T.

1 Tuyen P. T.

1 Xuan T. D.

1 2017

1 2016

Wyniki wyszukiwania

Identifying the grain chalkiness gene using molecular marker techniques in rice (Oryza sativa L.)

Correlation among agro-morphological variation and genetic diversity of rice (Oryza sativa L.) under drought stress