Wyniki wyszukiwania

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Genetic investigation for the characterization of three indigenous pig breeds of southern Italy: advantages and prospects

100%

Rossetti C., Perucatti A., Mottola F., Incarnato D., Genualdo V.

Animal Science Papers and Reports

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2021

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tom 39

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nr 2

2

Development of a sequential multicolor-FISH approach with 13 chromosome-specific painting probes for the rapid identification of river buffalo (Bubalus bubalis, 2n = 50) chromosomes

86%

Pauciullo A., Perucatti A., Iannuzzi A., Incarnato D., Genualdo V., Di Berardino D., Iannuzzi L.

Journal of Applied Genetics

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2014

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tom 55

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nr 3

3

Chromosomal assignment of R-spondin genes in the donkey (Equus asinus, 2n equals 62)

86%

De Lorenzi L., Genualdo V., Perucatti A., Pia Di Meo G., Molteni L., Iannuzzi L., Parma P.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

R-spondins constitute a recently discovered small family of growth factors, and the evidence of their role in several developmental pathways is growing fast. In this work we describe the chromosomal location of the four RSPO genes in the donkey. Using horse BACs, we localized RSIPO1 on EAS 5q23, RSPO2 on EAS 12q13, RSPO3 on EAS 24q26, and RSPO4 on EAS 15p13. Moreover, RSPO2, RSPO3, and RSPO4 are the first genes mapped on donkey chromosomes 12, 24, and 15, respectively.

4

Chromosome fragility in river buffalo cows exposed to dioxins

72%

Genualdo V., Perucatti A., Iannuzzi A., Di Meo G. P., Spagnuolo S. M., Caputi-Jambrenghi A., Coletta A., Vonghia G., Iannuzzi L.

Journal of Applied Genetics

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2012

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tom 53

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nr 2

Fifty river buffalo (Bubalus bubalis, 2n=50) cows reared in two different provinces of Campania (southern Italy) underwent cytogenetic investigations to ascertain possible differences in their chromosome stability. One group (Caserta province) was under legal sequestration due to the presence in the milk mass of higher mean values of dioxins [21.79 pg/g of fat as sum of polychloro-dibenzo-dioxins (PCDDs), polychloro-dibenzo-furans (PCDFs) and dioxin-like polychlorobiphenyls (DL-PCBs)] than both those permitted (6.0 pg/g of fat as WHO-TEQ) and those (1.3 pg/g of fat as WHO-TEQ) observed in the control group raised in Salerno province. Two types of peripheral blood cell cultures were performed: without (normal cultures for the chromosome abnormality (CA) test: chromatid breaks, chromosome breaks, fragments) and with the addition of BrdU for the sister chromatid exchange (SCE) test). The CA test revealed a significantly (P<0.01) higher chromosome fragility in the exposed cows compared to the control. Indeed, mean values of CA/cell were 1.26±1.15 in exposed cows and 0.37±0.71 in the control. Mean SCE was higher in exposed cows (8.50±3.35) than that (8.29±3.51) found in the control but the difference was not significant. Comparison within the same group of cows at first (FL) and multiple (ML) lactations revealed significantly (P<0.01) higher mean values of CA/cell in exposed ML-cows vs FL-cows while no statistical differences were found between ML-cows and FL-cows in the control farm. By contrast, significantly (P<0.01) higher mean values of SCE were found in both groups of FL-cows versus ML-cows. Comparisons with other previous studied species (sheep and cattle) were also performed.

5

Fluorescence in situ hybridization mapping of six loci containing genes involved in the dioxin metabolism of domestic bovids

59%

Genualdo V., Spalenza V., Perucatti A., Iannuzzi A., Di Meo G. P., Caputi-Jambrenghi A., Vonghia G., Rasero R., Nebbia C., Sacchi P., Iannuzzi L.

Journal of Applied Genetics

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2011

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tom 52

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nr 2

Six loci containing genes involved in the dioxin metabolism (ARNT, AHR, CYP1A1, CYP1A2, CYP1B1 and AHRR) were assigned, for the first time, to cattle (Bos taurus, 2n = 60, BTA), river buffalo (Bubalus bubalis, 2n = 50, BBU), sheep (Ovis aries, 2n = 54, OAR) and goat (Capra hircus, 2n = 60, CHI) chromosomes by comparative FISH-mapping and R-banding using bovine BAC-clones. The following chromosome locations were found: ARNT to BTA3q21, BBU6q21, OAR1p21 and CHI3q21, AHR to BTA4q15, BBU8q15, OAR4q15 and CHI4q15; CYP1A1 and CYP1A2 to BTA21q17, BBU20q17, OAR18q17 and CHI21q17; CYP1B1 to BTA11q16, BBU12q22, OAR3p16 and CHI11q16, AHRR to BTA20q24, BBU19q24, OAR16q24 and CHI20q24. All loci were mapped at the same homoeologous chromosomes and chromosome bands of the four bovid species. Comparisons with corresponding human locations were also reported.

Ograniczanie wyników

4 Journal of Applied Genetics

1 Animal Science Papers and Reports

5 Genualdo V.

5 Perucatti A.

4 Iannuzzi L.

3 Iannuzzi A.

2 Caputi-Jambrenghi A.

2 Di Meo G. P.

2 Incarnato D.

2 Vonghia G.

1 Coletta A.

1 De Lorenzi L.

1 Di Berardino D.

1 Molteni L.

1 Mottola F.

1 Nebbia C.

1 Parma P.

1 Pauciullo A.

1 Pia Di Meo G.

1 Rasero R.

1 Rossetti C.

1 Sacchi P.

1 Spagnuolo S. M.

1 Spalenza V.

1 2021

1 2014

1 2012

1 2011

1 2010

Genetic investigation for the characterization of three indigenous pig breeds of southern Italy: advantages and prospects

Development of a sequential multicolor-FISH approach with 13 chromosome-specific painting probes for the rapid identification of river buffalo (Bubalus bubalis, 2n = 50) chromosomes

Chromosomal assignment of R-spondin genes in the donkey (Equus asinus, 2n equals 62)

Chromosome fragility in river buffalo cows exposed to dioxins

Fluorescence in situ hybridization mapping of six loci containing genes involved in the dioxin metabolism of domestic bovids