P. ixocarpa hairy root cultures were obtained after the transformation with A. rhizogenes strain ATCC 15834. The ability of P. ixocarpa hairy roots to biotransform HQ to arbutin was examined. The roots were treated 3 times with the same HQ concentration on 3 consecutive days or every 3 days. Despite these differences the highest arbutin yield and the highest biotransformation ratio were similar in both variants, 13.1 and 14.4 mg·25 cm⁻³ of the cultures and 67.6% and 70.6%, respectively. However, in the case of shorter intervals between treatments the highest levels of these parameters were achieved earlier. Multiple treatment of lower HQ concentration reduced its harmful effects on root biomass growth.