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1

Dissolved heavy metals distribution and risk assessment in the Le’an River subjected to violent mining activities

100%
Jiang Y., Xie H., Zhang H., Xie Z., Cao Y.
Polish Journal of Environmental Studies
|
2018
|
tom 27
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nr 4
Surface water samples were collected from 24 sampling sites throughout the Le’an River during wet and dry seasons. The concentrations of dissolved heavy metals were determined by inductively coupled plasma-mass spectrometry (ICP-MS). The sources of dissolved heavy metals in the Le’an River were investigated based on the seasonal and spatial variations. The results demonstrated that significant seasonality of dissolved heavy metals concentrations were found in the Le’an River, and high concentrations in dry season due to the precipitation dilution effect. Spatially, higher concentrations of selected dissolved heavy metals were distributed in the mining area, which was significantly influenced by mining activities. It was found that sites within the mining area suffered from serious pollution based on the calculated HPI and MPI. According to human health risk assessment, it was indicated that As, Co, and Cd were the major contributors to exposure to local inhabitants. The exposure risks of the adults were less sensitive compared to the children, and oral ingestion was the primary exposure pathway. Multivariate statistical analyses revealed that different groups of heavy metals were characteristics of the disparate source associated with mineral exploration, urban and agricultural activities, and geogenic origins. Hierarchical agglomerative CA grouped all the sampling sites into three clusters based on the data set of exposure risk to human health and aquatic life. In cluster 2, concentrations of Cd and As were higher than drinking water quality of WHO and Chinese standards.
2

The expression of a new HD-Zip II gene, MSHB1, involving the inhibitory effect of thidiazuron on somatic embryogenic competence in alfalfa (Medicago sativa L. cv. Jinnan) callus

86%
Hu X., Zhang C.-R., Xie H., Huang X., Chen Y.-F., Huang X.-L.
Acta Physiologiae Plantarum
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2012
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tom 34
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nr 3
Homeodomain leucine zipper (HD-Zip) proteins play important roles in plant development. In this study, we not only identified and characterized a new HD-Zip II gene, designated as MSHB1 (HM114227), from alfalfa (Medicago sativa L. cv. Jinnan) callus treated with thidiazuron (TDZ) which reduced the embryogenic competence of the callus, but also presented the first evidence that MSHB1 is involved in the inhibitory effect of TDZ on somatic embryogenic competence in alfalfa callus. The full-length cDNA was 1,578 bp with an open reading frame of 1,023 bp, encoding a predicted protein of 340 amino acid residues, plus three introns. MSHB1 was strongly expressed in the callus treated with TDZ, but was only slightly detected in the leaf and petiole. TDZ treatment significantly decreased the frequency of somatic embryogenesis in the callus, but up-regulated MSHB1 expression during callus induction, callus maintenance and somatic embryo induction. These results suggest that the inhibitory effect of TDZ on embryogenic competence of alfalfa callus might be mediated by the regulation of MSHB1 expression.
3

The heterogeneity of cell subtypes from a primary culture of human amniotic fluid

86%
Zhang S., Geng H., Xie H., Wu Q., Ma X., Zhou J., Chen F.
Cellular and Molecular Biology Letters
|
2010
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tom 15
|
nr 3
424-439
Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.
4

Physiological responses of Scirpus validus to nitrate stress

86%
Li X., Li H., Shi G., Xiao M., Li C., Xie H.
Polish Journal of Environmental Studies
|
2020
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tom 29
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nr 1
5

The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells

59%
Li X., Xie H., Jiang Q., Wei G., Lin L., Li C., Ou X., Yang L., Xie Y., Fu Z., Liu Y., Chen D.
Cellular and Molecular Biology Letters
|
2017
|
tom 22
6

Effects of Abietinella abietina extracts on the germination and seedling emergence of Picea crassifolia: results of greenhouse experiments

59%
Wang Q., Zhao C., Gao Y., Gao C., Qiao Y., Xie H., Wang W., Yuan L., Liu J., Ma W., Li W.
Polish Journal of Ecology
|
2016
|
tom 64
|
nr 3
Moss cushion plays an important role in recruitment of vascular plants. In this study, we examined the effects of water soluble extracts (WSE) of Abietinella abietina on germination of Qinghai spruce (Picea crassifolia) and the effects of moss substrates (A. abietina) on seedling emergence of P. crassifolia in two greenhouse experiments. We found that the WSE of moss affected germination of P. crassifolia in two ways: (1) the WSE reducing the risk of fungal infection to the seeds, improve the germination rate; and (2) the WSE affects germination by its concentration, that is, low concentrations of WSE (i.e., 0.5 mg ml⁻¹ and 5 mg ml⁻¹) stimulate, while high concentrations (i.e., 50 mg ml⁻¹) inhibit it. In seedling emergence experiments, we detected that the moss substrates (both thick and thin mosses) facilitate the emergence of P. crassifolia in dry and intermediate moisture conditions, but inhibit it in moist conditions. In bare soil, the P. crassifolia emergence was also controlled by moisture conditions; the highest (60%) and the lowest (35%) emergence occurred in the moist conditions and dry conditions, respectively. We also found that P. crassifolia seedlings were thinner and taller, but their number higher in moss cushion than in bare soil. Thus we conclude that there is nurse effect of A. abietina cushion on recruitment of P. crassifolia in both dry and intermediate moisture conditions.
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