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The effects of six emergent macrophytes (Typha orientalis, Acorus calamus, Oenanthe javanica, Scirpus validus, Sagittaria sagittifolia, and Pontederia cordata) on the growth of two strain Microcystis aeruginosa were studied under co-culture conditions. And the sensitivities of unicellular and colonial Microcystis strains to six emergent macrophytes were compared using an exudation experiment. Based on laboratory experiments, T. orientalis, A. calamus, O. javanica, S. validus, S. sagittifolia, and P. cordata had strong inhibitory effects on growth of unicellular M. aeruginosa, while only A. calamus and P. cordata show obvious growth inhibition on colonial M. aeruginosa. When the biomass density was 20 g FW·L⁻¹, the growth inhibition rate of unicellular M. aeruginosa can exceed 90% for all of the six emergent macrophytes. When macrophytes coexisted with the colonial M. aeruginosa, only A. calamus, P. cordata, and S. sagittifolia showed the growth inhibition of algae. Maximal inhibition of Chl a growth was 75% (p<0.05) for A. calamus, 69% (p<0.05) for P. cordata, and 40% for S. sagittifolia at 45 g FW·L⁻¹ on day 15. The results of the exudation experiment indicated that there were no significant differences between control and treatment of Chl a concentrations of colonial M. aeruginosa for all of the six macrophyte exudations on days 6 and 12. While after 6 d incubation in 100% and 50% macrophyte exudations (40 g FW·L⁻¹), the cell densities of unicellular M. aeruginosa in control were obviously higher than all those in treatment (p < 0.05). The maximal algal growth inhibition (89.62%) of unicellular M. aeruginosa was achieved in 100% exudation of A. calamus on day 6 (p < 0.05). So according to the results of exudation experiments, the unicellular M. aeruginosa was more sensitive than the colonial strain to six emergent macrophytes. And this different sensitivity between Microcystis species probably correlated positively with colony size.
In this study, Beta vulgaris L. var. cicla was grown in cadmium-contaminated soil in a greenhouse. Regulated deficit irrigation was applied using three different irrigation levels (T1: 300 L, T2: 200 L, T3: 100 L per block during each irrigation event during the organogenesis stage; T1 was the control) to examine the effects on phytoremediation efficiency. According to the experimental results, the regulated deficit irrigation treatment (T2) decreased the Beta vulgaris L. var. cicla shoot biomass by 15.8%, increased the Cd concentration in the shoots by 23%, and maintained a constant root-shoot ratio. By contrast, T3 decreased the Beta vulgaris L. var. cicla shoot biomass by 33.0%, decreased the Cd concentration in shoots by 9.8%, and increased the root-shoot ratio by 62.8%. The Cd remediation potential efficiency (PE) of treatment T2 was 5.42 g ha-1 – i.e., 39.7% higher than that of T1 and 61.8% higher than that of T3. This study indicated that regulated deficit irrigation can be used to enhance Cd phytoremediation and save water, but should be applied in a suitable way.
A dynamic habitat and population model has been developed and applied to the tailwater below Glen Canyon Dam to estimate the dam operation’s effects on rainbow trout (Oncorhynchus mykiss) habitat and population dynamics. Water depth, flow temperature, flow velocity, and substrate types were used as the suitability parameters and to evaluate these indicators. Historic hydraulic data from 1991 to 2009 were analyzed to determine the minimum, mean, and maximum flow rates that were used to represent the habitat suitability index (HSI) and overall suitability index (OSI) of the rainbow trout fry, adult, and spawning life stages, respectively. Fish abundance and the simulation results were also compared with observed fish numbers. Results indicated that under the historic dam operation, the habitat suitability level in the Colorado River was not suitable for fry and spawning rainbow trout, but very suitable for adult rainbow trout. It is indicated that high HSI levels do not mean high fish abundance. It can also be seen that overall rainbow trout abundance decreased during the period 1991 to 2009.
With the development of automation in ports, the video surveillance systems with automated human detection begun to be applied in open-air handling operation areas for safety and security. The accuracy of traditional human detection based on the video camera is not high enough to meet the requirements of operation surveillance. One of the key reasons is that Histograms of Oriented Gradients (HOG) features of the human body will show great different between front & back standing (F&B) and side standing (Side) human body. Therefore, the final training for classifier will only gain a few useful specific features which have contribution to classification and are insufficient to support effective classification, while using the HOG features directly extracted by the samples from different human postures. This paper proposes a two-stage classification method to improve the accuracy of human detection. In the first stage, during preprocessing classification, images is mainly divided into possible F&B human body and not F&B human body, and then they were put into the second-stage classification among side human and non-human recognition. The experimental results in Tianjin port show that the two-stage classifier can improve the classification accuracy of human detection obviously
Environmental heterogeneity is a primary factor in determining soil microbial spatial patterns. However, few studies have demonstrated a link between environmental heterogeneity and the spatial patterns in free-living nitrogen-fixing bacteria, which are important in nitrogen cycling. Here, quantitative polymerase chain reaction and terminal restriction fragment length polymorphism were used to determine the spatial distribution of free-living nitrogen-fixing bacteria along a slope gradient. Plant communities were evaluated through field surveys, and basic soil properties were measured in the laboratory. Soil properties (e.g., soil available phosphorus, soil organic carbon, and total nitrogen) were higher in upper- and middle -slope soils than in lower-slope soils, while bacterial abundance was the opposite. Bacterial and plant communities all varied along the slope gradient. Redundancy analysis revealed that bacterial community composition was closely linked to pH, soil organic carbon, available phosphorus, and total nitrogen. These results indicate that free-living nitrogen-fixing bacterial communities show strong spatial structure along a slope gradient, and emphasize the importance of soil heterogeneity in affecting bacterial spatial patterns in a karst region.
The occurrence of 22 antibiotics, including eight quinolones, nine sulfonamides and five macrolides in water and sediment from Zizhuyuan Lake has been investigated in Beijing, China. Sediment samples were extracted by pressurized liquid extraction (PLE). Aqueous extracts from PLE and water samples were concentrated by solid-phase extraction (SPE) and detected by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS). Antibiotics were detected in concentrations of up to 20 ng/L in water samples and 52.1 μg/kg in sediments. Quinolones were the most prevalent contaminants in water body, accounting for 63.4%, while quinolones and macrolides were prominent in sediments, accounting for 55.1% and 41.7%, respectively. The environmental risk assessment revealed that some antibiotics, in particular Ofloxacin (OFL) and Sulfamethoxazole (SMX), could cause high damage to algae and plants in the aquatic ecosystem.
Polygalacturonase-inhibiting proteins (PGIPs) are multifunctional proteins related to plant autoimmunity and belong to the plant extracellular leucine-rich repeat (eLRR) protein superfamily. PGIPs play a role in host defense in many plants. In the present study, a novel PGIP gene, PpPGIP was isolated from Pyrus pyrifolia Nakai cv Huobali. The nucleotide sequence of PpPGIP was highly homologous with PGIPs from other plant species and the protein encoded by PpPGIP has several conserved LRR domains. The putative protein PpPGIP was closely clustered with several PGIPs from horticultural plants on the phylogenetic tree. The constructed homology model of PpPGIP indicated that the main-chain conformation and the folding patterns of PpPGIP were highly similar to structural features of PvPGIP2 from Phaseolus vulgaris. The expression levels of PpPGIP in healthy tissue and organ of ‘Huobali’ were analyzed with RT-PCR, and PpPGIP accumulated a little in young leaves, but PpPGIP was expressed abundantly in the pericarp of ‘Huobali’ fruits. Furthermore, in order to verify the function of PpPGIP, the constitutive plant expression vector of PpPGIP was constructed and transferred into tobacco (Nicotiana tabacum L. cv Xanthi). The Southern blot and real-time PCR analyses demonstrated that the PpPGIP gene was integrated into the genome of the tobacco transformants and highly expressed in the transgenic lines. The antifungal activity of PpPGIP was detected in vitro plates, and the crude protein extract of transgenic tobacco plants inhibited the hyphal growth of Phomopsis sp., Alternaria sp., Penicillium sp., and Aspergillus niger in different degrees.
Using open top chambers, the effects of elevated O₃ (80 nmol mol⁻¹) and elevated CO₂ (700 µmol mol⁻¹), alone and in combination, were studied on young trees of Quercus mongolica. The results showed that elevated O₃ increased malondialdehyde content and decreased photosynthetic rate after 45 days of exposure, and prolonged exposure (105 days) induced significant increase in electrolyte leakage and reduction of chlorophyll content. All these changes were alleviated by elevated CO₂, indicating that oxidative stress on cell membrane and photosynthesis was ameliorated. After 45 days of exposure, elevated O₃ stimulated activities of superoxide dismutase (SOD, EC 1.15.1.1) and ascorbate peroxidase (APX, EC 1.11.1.11), but the stimulation was dampened under elevated CO₂ exposure. Furthermore, ascorbate (AsA) and total phenolics contents were not higher in the combined gas treatment than those in elevated O₃ treatment. It indicates that the protective effect of elevated CO₂ against O₃ stress was achieved hardly by enhancing ROS scavenging ability after 45 days of exposure. After 105 days of exposure, elevated O₃ significantly decreased activities of SOD, catalase (CAT, EC 1.11.1.6) and APX and AsA content. Elevated CO₂ suppressed the O₃-induced decrease, which could ameliorate the oxidative stress in some extent. In addition, elevated CO₂ increased total phenolics content in the leaves both under ambient O₃ and elevated O₃ exposure, which might contribute to the protection against O₃-induced oxidative stress as well.
The presence of perfluorinated compounds (PFCs) in ambient air particulates (PM2.5, PM10, and TSP) in Beijing was investigated in the current study. The results showed that PFBA, PFPeA, PFHpA, PFOA, PFNA, PFDA, PFUnA, PFDoA, and PFOS, were detected in all sizes of ambient air particulates. The concentrations of PFCs in TSP were slightly higher than those in PM10 and PM2.5. The concentrations of PFCs in PM2.5 were the lowest. The ΣPFCs contents in PM2.5, PM10 and TSP were 87.03-96.77, 99.82-101.41, and 118.69-141.87 ng/g, respectively. PFBA, PFPeA, and PFOA were the dominating class of PFCs in ambient air particulates.
In karst areas, in order to ascertain the relationship between soil active organic carbon fractions and soil nutrient elements, topsoil samples (the sampling points were set at 80-m intervals) were collected for the analysis of soil physicochemical properties. In the sampling area, land use was divided into upland, paddy, and shrub land. The results showed that dissolved organic C (DOC) and microbial biomass C (MBC) contents in shrub land soil were higher than those values in upland and paddy soils (p<0.05). The total nitrogen (TN) content in paddy was lower than in upland and shrub land (p<0.05). The mean value of total phosphorus (TP) in upland and shrub land was approximately 1.5 times higher than that in paddy. Available nitrogen (AN) in shrub land soil was higher than in upland, whereas Olsen-P was lowest in shrub land in all soils (p<0.05). The C:P and N:P ratios in upland and paddy were lower than in shrub land (p<0.05). At plot scale, random forest analysis revealed that pH and soil organic carbon (SOC) were the most important variables determining DOC content in croplands (upland and paddy) and shrub land, respectively. The accumulation of SOC accelerates the growth of microbial biomass in upland. In shrub land, increases in SOC and total nitrogen were in favor of microbial growth. At the small-watershed scale, C:P and N:P ratios had a significant and positive effect on the content of DOC and MBC, respectively. The results implied that high C and N availability, especially in combination with low P availability, is helpful for increasing soil microbial biomass.
Phoebe bournei commonly called nanmu is an important and endemic wood species in China, and its planting, nursing, and preserving are often affected by drought stress. Two-year-old P. bournei seedlings were subjected to water stress and recovery treatment to study their physiological and biochemical responses. Physiological and biochemical indices did not change when seedlings were subjected to mild water stress (˂15 days of water withholding). As drought stress intensified (>20 days of water withholding), malondialdehyde and electrolyte leakage increased, and chlorophyll and soluble protein decreased, indicating an increased oxidative stress induced by water deficit. Enhanced activities of superoxide dismutase (SOD) and peroxidase (POX), accumulation of free proline and total soluble sugar contribute to plant protection against the oxidative stress. However, SOD and POX decreased when seedlings were subjected to an extended drought. After 5 days of recovery, physiological and biochemical indices were not restored to the control level values except for leaf relative water content when the seedlings were subjected to more than 20 days water stress. These results demonstrate that P. bournei could enhance their ability to mitigate water stress effects by up-regulating antioxidant system and osmotic adjustment, but these two protective mechanisms were limited when seedlings were subjected to moderate and severe water stress. The threshold of water deficit to P. bournei seedlings is 15–20 days, and permanent damage will be induced if water status is not improved before this threshold. The results will provide some theoretical and practical guidance for nanmu afforestation and production.
Four-year-old Gingko (Ginkgo biloba L.) trees were exposed to ambient and elevated concentrations of CO₂ and O₃, and their combination for 1 year, using opentop chambers in Shenyang, China in 2006. Growth parameters and endogenous plant hormones were measured simultaneously over the experiment period. Elevated CO₂ increased leaf area and leaf dry weight but had no effect on shoot length, increased indole-3-acetic acid (IAA), gibberellins A₃ (GA₃), zeatin riboside (ZR), dihydrozeatin (DHZR) and isopentenyl-adenosine (iPA) content but decreased abscisic acid (ABA) content. Elevated O₃ significantly decreased leaf area, leaf dry weight, shoot length, and decreased IAA, GA₃, ZR content but increased ABA content and had a little effect on iPA, DHZR content. Elevated CO₂ + O₃ decreased IAA, iPA and DHZR content while increased ABA and GA₃ content in the early stage of the exposure and then decreased in the late stage. The evidence from this study indicates that elevated CO₂ ameliorated the effects of elevated ozone on tree growth, and elevated CO₂ may have a largely positive impact on forest tree growth while elevated O₃ will likely have a negative impact.
The arbuscular mycorrhizal fungi (AMF) enhance the resistance to pathogen infection in host plant. However, it is unclear how the AMF are involved in the systemic acquired resistance of host plant against pathogen. Here, an experiment was carried out to clarify the role of the AMF in soybean’s defense against the infection from pathogen Phytophthora sojae. It was found that the AMF contributed to the resistance of soybean against Phytophthora sojae by the release of hydrogen peroxide and by the accumulation of jasmonic acid in response to pathogenic invasion. Furthermore, the trade of nitrogen (N) from the fungus for carbon from the host was accelerated in the AM symbiosis in the defense reaction, which was indicated by the increased soluble sugar level, NO content and enzyme activities involved in N metabolism in the AM symbiosis.
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