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In 2007 and 2008 studies aimed to determine the effect of preparation belonging to carbamate compounds (Pirimor 500 WG), organophosphorus compounds (Diazol 500 EW), and quinazolin compounds (Magus 200 SC), on the oxygen consumption rate by adult beetles Anoplotrupes stercorosus were performed. Experiments were carried out under diverse ambient temperatures (14, 19, 24 i 29°C) using two ways of intoxication – contact intoxication or intoxication by ingestion of the biocide. In control insects the ambient temperature affected the oxygen demand only to a small extent. Usually, insecticide preparations which were used, markedly potentiated the oxygen consumption. In those experimental groups significant increases of oxygen consumption as the effect of ambient temperature elevation were noted. The mode of the intoxication influenced oxygen consumption only very slightly. The highest values of oxygen consumption were noted in animals treated by contact intoxication.
We examined the susceptibility of the worker honeybee Apis mellifera L. to pyrethroids. Bees were intoxicated by selected synthetic pyrethroids (active substances: beta-cyfl uthrin, deltamethrin, alphacypermethrin, lambda-cyhalothrin, esfenvalerate, and bifenthrin) and 72 hours after intoxication the number of surviving insects was determined. It was observed that the susceptibility of the honeybee to pyrethroids (expressed herein as the survival rate) was changing in diurnal rhythm, ranging from 100% (for a majority of substances in both seasons) to 11.1±0.37% (deltamethrin) and 11.1±0.73% (esfenvalerate) in spring, and 11.1±0.73% (esfenvalerate) and 4.6±0.17% (bifenthrin) in summer. The lowest susceptibility of bees to pyrethroids occurred during the night, when the honeybees were characterized by reduced motility and did not fl y out for foraging, whereas the highest susceptibility was observed during the day, a natural time for seeking food. Along with diurnal changes in the susceptibility described above, differences dependent on the seasons in which the intoxication took place were also observed. Insects intoxicated in summer were less susceptible to pyrethroids than those intoxicated in spring.
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