Wyniki wyszukiwania

1

Wplyw stanu redoks komorki na aktywacje czynnikow transkrypcyjmych i ekspresje genow

100%

Cisowski J.

Postępy Biologii Komórki. Suplement

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2001

|

tom 16

43-59

2

Soczysty i kolorowy sposób na zdrowie

63%

Spillane D., Cisowski J.

Przemysł Fermentacyjny i Owocowo-Warzywny

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2009

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tom 53

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nr 05

3

IL-1-mediated inhibition of IL-6-induced STAT3 activation is modulated by IL-4, MAP kinase inhibitors and redox state of HepG2 cells

51%

Cisowski J., Zarebski A., Koj A.

Folia Histochemica et Cytobiologica

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2002

|

tom 40

|

nr 4

4

The role of nitric oxide synthase, superoxide dismutase and heme oxygenase-1 in the synthesis of vascular endothelial growth factor

45%

Dulak J., Grzenkowicz J., Cisowski J., Podhajska A., Jozkowicz A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

5

Overexpression of biliverdin reductase enhances heme oxygenase expression, activates ERK1, JNK1 and p38 kinases and ensure partial protection against cellular stress

39%

Florczyk U., Golda S., Zieba A., Cisowski J., Jozkowicz A., Dulak J.

Acta Biochimica Polonica

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2007

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tom 54

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nr Supplement 4

6

Mechanisms of of vascular endothelial growth factor synthesis by hydrogen peroxide and overexpression of copper-zinc superoxide dismutase: role of heme oxygenase-1

39%

Cisowski J., Grzenkowicz-Wydra J., Cheng S., Agarwal A., Jozkowicz A., Podhajska A., Dulak J.

Acta Biochimica Polonica

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2003

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tom 50

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nr Supplement 1

7

Prostaglandin-J2 upregulates expression of matrix metalloproteinase-1 independently of activation of peroxisome proliferator-activated receptor-gamma

39%

Jozkowicz A., Huk I., Nigisch A., Cisowski J., Weigel G., Dulak J.

Acta Biochimica Polonica

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2003

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tom 50

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nr 3

Peroxisome proliferator-activated receptor-y (PPARy) is a ligand-inducible nuclear receptor that functions as a transcription factor involved in lipid metabolism, inflammatory response and angiogenesis. The most potent endogenous PPARγ activator is 15-deoxy-Δ12,14,prostaglandin-J2 (15d-PGJ2), whereas synthetic ligands include the oral antidiabetic drugs thiazolidinediones (TZDs). Activation of PPARγ was reported to decrease the synthesis of matrix metalloproteinases (MMPs) in vascular smooth muscle cells and macrophages. We aimed to investigate the effect of PPARy ligands on expression of MMP-1 and urokinase plasminogen activator (uPA) in human microvascular endothelial cells (HMEC-1). We found that treatment of HMEC-1 with 15d-PGJ2 increased the synthesis of MMP-1 protein up to 168% comparing to untreated cells. TZDs (ciglitazone and troglitazone), more potent activators of PPAR in HMEC-1, did not influence MMP-1 production, arguing against the involvement of PPAR in this process. Importantly, the stimulatory effect of 15d-PGJ2 was reversed by the antioxidant N-acetyl-cysteine (NAC), suggesting a contribution of oxidative stress. We demonstrated also that 15d-PGJ2 did not change the activity of MMP-1 promoter, but increased the stability of MMP-1 mRNA. In contrast, 15d-PGJ2 very potently inhibited the synthesis of uPA. This effect was in part mimicked by ciglitazone and troglitazone implying an involvement of PPAR. Accordingly, NAC did not modify the inhibitory effect of 15d-PGJ2 on uPA expression. In conclusion, we postulate that 15d-PGJ2 may differently regulate the synthesis of proteases involved in angiogenesis: it upregulates MMP-1 expression in HMEC-1 through induction of oxidative stress, and inhibits uPA synthesis partly by activation of PPARγ.

8

Regulacja ekspresji genow w wektorach plazmidowych: system zalezny od doksycykliny i regulowany przez niedotlenienie

33%

Golda S., Kucharzewska P., Cisowski J., Florczyk U., Zagorska A., Jazwa A., Loboda A., Jozkowicz A., Dulak J.

Biotechnologia

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2007

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nr 3

82-97

9

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Effect of plant extracts on angiogenic activities of endothelial cells and keratinocytes

33%

Loboda A., Cisowski J., Zarebski A., Jazwa A., Rivera Nunez D., Kypriotakis Z., Heinrich M., Dulak J.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 1

125-137

Numerous bioactive chemical compounds of plant origin may influence the angiogenic activity of various cell types and may thus affect the formation of blood vessels. Here we present the angiogenic effects of extracts of edible plants collected in Crete, Southern Italy and Southern Spain. Extracts have been applied to cultured human microvascular endothelial cells (HMEC-1), human umbilical vein endothelial cells (HUVEC) and human keratinocytes (HaCaT). About half out of 96 extracts exerted an inhibitory effect on HMEC-1 proliferation. Additionally, we have noted the inhibitory effects of extracts on HUVEC differentiation on a Matrigel layer. None of the extracts showed a stimulatory activity. The extract of Thymus piperella exerted moderate inhibitory effect on cobalt-chloride induced VEGF synthesis, however, CoCl2-induced activation of hypoxia responsive element of VEGF promoter was significantly attenuated only by extract of Origanum heracleoticum. Our study indicates that extracts of local food plants, of potential value as nutraceuticals, contain chemical compounds which may inhibit angiogenesis. Demonstration of their real influence on human health requires, however, extensive animal studies and controlled clinical investigations.

Ograniczanie wyników

Wplyw stanu redoks komorki na aktywacje czynnikow transkrypcyjmych i ekspresje genow

Soczysty i kolorowy sposób na zdrowie

IL-1-mediated inhibition of IL-6-induced STAT3 activation is modulated by IL-4, MAP kinase inhibitors and redox state of HepG2 cells

The role of nitric oxide synthase, superoxide dismutase and heme oxygenase-1 in the synthesis of vascular endothelial growth factor

Overexpression of biliverdin reductase enhances heme oxygenase expression, activates ERK1, JNK1 and p38 kinases and ensure partial protection against cellular stress

Mechanisms of of vascular endothelial growth factor synthesis by hydrogen peroxide and overexpression of copper-zinc superoxide dismutase: role of heme oxygenase-1

Prostaglandin-J2 upregulates expression of matrix metalloproteinase-1 independently of activation of peroxisome proliferator-activated receptor-gamma

Regulacja ekspresji genow w wektorach plazmidowych: system zalezny od doksycykliny i regulowany przez niedotlenienie

Effect of plant extracts on angiogenic activities of endothelial cells and keratinocytes