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The aim of the present paper was to determine the effect of bacteriocins produced by Lactobacillus strains isolated from pickled vegetables on Gram-negative pathogenic rods. The antibacterial effect of bacteriocins was determined with the diffusion method, employing the well technique. Incubation was carried out for 24 hours, under the optimum conditions for test strains, and then growth inhibition zones around wells were observed. The results obtained show that individual Lactobacillus strains produce antibacterial substances - bacteriocins, characterized by different activity towards selected strains of Yersinia enterocolitica, Salmonella E. coli O 157.H7 and E. coli K88. Test strains were more sensitive to bacteriocins synthesized by Lactobacillus plantarum, as their growth was inhibited at the activity level 4-32 AU/mL. Bacteriocins of Lactobacillus brevis strains inhibited their growth at 128 and 256 AU/mL.
The aim of the present paper was to determine the level of resistance of Lactobacillus bacteria isolated from sauerkraut and pickled cucumbers to antibiotics. Lactic fermentation bacteria were isolated on MRS, according to the methodology elaborated by Rogos. Tests API 50 CH (produced by the company bioMerieux) were applied for identification. Resistance to antibiotics was determined employing the diffusion method. It was found that the resistance of Lactobacillus rods isolated from pickled products to antibiotics depended on the strain species and source of its origin. All strains of Lactobacillus planiarum and Lactobacillus brevis were resistant to nalidixic acid, 41.3% of strains turned out to be resistant to kanamycin, while only 3.4% to neomycin. As concerns polypeptide antibiotics, 27.5% of the strains tested were resistant to Colistin. All strains of lactic fermentation bacteria isolated from pickled vegetables were sensitive to ß-lactam antibiotics, riphamycins, furan derivatives and chloramphenicol.
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