To date, there is no complementary panel of markers that could be used internationally in cases of disputed parentage. The Department of Animal Cytogenetics and Molecular Genetics of the National Research Institute of Animal Production has developed a complementary panel of microsatellite loci, which is used when the standard panel is insufficient for parentage determination. Among the markers tested, the following panel of microsatellite sequences was chosen to complement the panel of markers available at the National Research Institute of Animal Production: CSRM60, ILSTS065, CSSM066, BM1818, INRA072, AGLA293, INRA222, INRA092 and HUJI177. The aim of the study was to determine the polymorphism of the panel of STR markers by estimating the degree of expected (HE) and observed (HO) heterozygosity, the polymorphic information content (PIC), the power of discrimination (PD) and the probability of exclusion (PE1 and PE2) The 63 alleles identified at 9 loci in 174 head of Simmental cattle were used to estimate the degree of heterozygosity and polymorphism in the cattle population examined. A high degree of observed heterozygosity and polymorphism (over 60%) was found in all the markers analysed except AGLA293 and CSSM66. The power of discrimination, calculated for each marker, was PD>0.8. Lower values of this parameter were observed only for AGLA293 and CSSM66. The probability of paternity exclusion on the basis of the newly developed panel of 9 STR loci was 97.74% when the genotype of one parent was known and 99.86% when the genotypes of both parents were known.
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