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1

Assessing thep hytotoxicity of tannery waste-contaminated soil on Zea mays (Lin) growth

100%
Yasin M., Faisal M.
Polish Journal of Environmental Studies
|
2013
|
tom 22
|
nr 6
Four chromium-resistant bacterial strains (Bacillus pumilus-CrK08, Cellulosimicrobium cellulansCrK16, Exiguobacterium-CrK19, and Bacillus cereus-CrK20) that could resist up to 25 mg·ml⁻¹ of Cr (VI) were used to inoculate Zea mays (Lin) seeds grown in tannery effluent-contaminated and normal garden soils. Overall, plants growing in tannery-contaminated soil showed slow leaf growth, 40% reduction in shoot length, 73% reduction in dry biomass, burning of leaf margins, delayed flower bud initiation, and feminization of male flowers compared to control. Zea mays (Lin) plants growing in tannery-contaminated soil showed an increase in acid phosphatase activity (14-26%), soluble proteins content (17-38%), chlorophyll a (34%) and b contents (70%), and a decrease in peroxidase (19%) and carotenoid contents (50%) compared to control. Non-inoculated plants have higher chromium uptake (114 mg/kg) as compared to inoculated (49.4 mg/kg).
2

Desiccation-tolerant rhizobacteria from Cholistan desert, Pakistan, and their impact on Zea mays L.

81%
Raza F. A., Amin A., Faisal M.
Polish Journal of Environmental Studies
|
2015
|
tom 24
|
nr 4
Five desiccation-tolerant rhizobacteria (Brevibacterium frigoritolerans-LPS1B, Bacillus subtilis-CHFT15, B. subtilis-CHFT12, B. subtilis-CH13, and Pseudomonas stutzeri-CHP413A) isolated from Pakistan’s Cholistan desert were characterized on the basis of morphological, biochemical, and 16S rDNA ribotyping. The desiccation tolerance was checked at various relative humidity levels (5, 23, and 100%) for a period of 1-40 days. Heavy metal and antibiotic resistance, auxin, cytokine, siderophore, hydrogen cyanide production, and phosphate solubilization of select bacterial isolates was also investigated. Pot experiments with corn in sandy and pure soil were also carried out to check the plant growth-promoting potential of select strains after 90 days of growth. After harvest, various growth parameters like seed germination, root and shoot length, number of leaves, dry weight per gram fresh weight, and chlorophyll contents were determined. The inoculation of P. stutzeri-CHP413A resulted in 3, 33,12, and 37% increases in seed germination, number of leaves, shoot and root length, and dry weight·g⁻¹ fresh weight, respectively, in sandy soil (p<0.05).
3

Chromate-reducing profile of bacterial strains isolated from industrial effluents

81%
Rizvi F. Z., Kanwal W., Faisal M.
Polish Journal of Environmental Studies
|
2016
|
tom 25
|
nr 5
Initially 73 chromium(VI)-resisting bacteria were isolated from nine samples collected from three industrial cities (Kasur, Kalashahkaku, and Sialkot) of Pakistan. Eleven strains with the highest chromium resistance also were selected. Among these highly resistant selected isolates (MTC ≥ 250 mM), AM81 (Cellulosimicrobium cellulans) showed the highest MTC of 375 mM against Cr(VI). Biochemical characterization was used to identify the families of bacteria after initial screening. Four isolates shared origin with Staphylococcaceae — three each with Promicromonosporaceae and Microbacteriaceae, and a single strain was related to Bacillales Family XII incertaesedis. 16S rRNA was used for species identification and found KM2 to be Leucobacter chironomid, KS1W; Microbacterium sp., SIS21 and KSKE42; Staphylococcus saprophyticus, SIS22; Staphylococcus sciuri, SIS51; Staphylococcus xylosus, MWM81, AM81, and KSKE3; Cellulosimicrobium cellulans, MWM82; and Microbacterium paraoxydans, KSKE41 was Exiguobacterium profundum. Strains tolerated the stress of other heavy metals (cadmium, mercury, copper, zinc, arsenic, and manganese) to variable extant along with chromium(VI). Antibiotic susceptibility was found to be more for streptomycin (10 μg ml-1) and the least susceptibility was observed for kanamycin (30 μg ml-1).
4

Encapsulation of microcuttings for propagation and short-term preservation in Ruta graveolens L.: a plant with high medicinal value

81%
Ahmad N., Faisal M., Fatima N., Anis M.
Acta Physiologiae Plantarum
|
2012
|
tom 34
|
nr 6
Synthetic seeds technology is a potential tool for an efficient and cost-effective clonal propagation system. In the present study, synthetic seeds were produced by encapsulating nodal segments (synthetic or synseeds) of Ruta graveolens in calcium alginate gel. The best gel complex was achieved using 3 % sodium alginate and 100 mM Cal₂.2H₂O. Maximum conversion response of synthetic seeds into plantlets was obtained on MS medium supplemented with 10 µM 6-benzyladenine (BA) and 2.5 µM α-naphthalene acetic acid (NAA). Encapsulated nodal segments could be stored at low temperature (4 °C) up to 4 weeks with a survival frequency of 86.7 %. The regenerated shoots rooted on MS medium containing 0.5 µM indole-3-butyric acid (IBA). Well-developed plantlets with proper root and shoot system from encapsulated nodal segments were hardened off successfully with 90 % survival rate. The high frequency of plant re-growth (conversion) from alginate-coated nodal segments coupled with high viability percentage after 4 weeks of storage is highly encouraging for the exchange of R. graveolens genetic resources.
5

Chromium accumulation and toxicity in corn (Zea mays L.) seedlings

81%
Naseem S., Yasin M., Ahmed A., Faisal M.
Polish Journal of Environmental Studies
|
2015
|
tom 24
|
nr 2
Extensive use of chromate compounds in the last few decades has resulted in contamination of our environment. In the present study we have investigated the effects of two different concentrations (10, 20 µg ml⁻¹) of chromium salts (CrCl₃, K₂CrO₄ K₂Cr₂O₇) on the growth of Zea mays L. As concentrations of chromium salts (CrCl₃, K₂CrO₄, K₂Cr₂O₇) increased, there was a significant decrease in seed germination ( 10-24%), shoot length (6-29%), root length ( 11 -33%), seedling length ( 16-24%), fresh weight of seedlings (17-67%) and increase in dry weight per seedling (3-15%), chromium content, acid phosphatases content (215-707%), and peroxidases activity (129-200%) of Zea mays plants compared to control treatment. In all treatments, the effect of hexavalent salts (K₂CrO₄ andK₂Cr₂O₇) was more severe on plant growth compared to trivalent Cr salts (CrCl₃). Zea mays plants have the ability to accumulate various chromium salts in their tissues and thus help to remediate the polluted soil.
6

Investigation of phytochemicals and in vivo antidiabetic evaluation of leaves and bark of Engelhardtia colebrookiana Lindl.

81%
Faisal M., Mushtaq W., Isthtiaq M., Qadeer M.
World Scientific News
|
2018
|
tom 91
7

Change in total phenolic content and antibacterial activity in regenerants of Vitex negundo L.

51%
Ahmad N., Khan M. I., Ahmed S., Javed S. B., Faisal M., Anis M., Rehman S., Umair S. M.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 03
A valuable medicinal plant, Vitex negundo L. has been investigated for its regeneration potential using shoot tip explants. Out of a range of concentrations of cytokinins [6-benzyl adenine (BA), 6-furfurylaminopurine, 2-isopentenyl adenine] used as supplement to Murashige and Skoog medium (MS), BA at 5.0 μM concentration proved best for multiple shoot induction yielding 3.60 ± 0.50 shoots after 8 weeks of culture. Inclusion of a low concentration of an auxin with optimal cytokinin concentration favoured shoot multiplication and the optimum response was observed on MS medium supplemented with BA (5.0 μM) along with a Naphthalene acetic acid (0.5 μM), where 65.0 ± 1.73 % cultures responded with a mean number of 4.80 ± 0.58 shoots per explants after 8 weeks of culture. Ex vitro rooting of in vitro derived microshoots was achieved upon dipping the cut ends of microshoots in 500 μM indole-3-butyric acid for 10 min followed by transfer to thermocol cups containing sterile soilrite. About 95 % of the plantlets survived the acclimatization procedure and were transferred to greenhouse and finally to field. Screening of the antibacterial activity and estimation of total phenolic content of ethanolic extracts of micropropagated plants were also carried out and compared with that of the mother plant.
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