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Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2

100%

Xia X. J., Wang L., Cheng L. K., Shen Z. Q., Li S. G., Wang J. L.

Polish Journal of Veterinary Sciences

2017

tom 20

nr 2

Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.

Ograniczanie wyników

1 Polish Journal of Veterinary Sciences

1 Cheng L. K.

1 Li S. G.

1 Shen Z. Q.

1 Wang J. L.

1 Wang L.

1 Xia X. J.

1 2017

Wyniki wyszukiwania

Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2