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The effect of LPS injections on non-specific immune response in affected pigeons

100%
Dudek K., Bednarek D., Siwicki A. K., Rokita E., Studzinski T.
Polish Journal of Veterinary Sciences
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2013
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tom 16
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nr 4
The aim of the study was to investigate the effect of LPS injections on non-specific mechanisms of immunity in pigeons. On the first day of observation the experimental birds (n=18) were intravenously injected with Escherichia coli LPS (10 μg/kg b.w.), while the control animals (n=6) received in the same way apyrogenic physiological saline. On the second and the third day of the experiment LPS in the same doses was injected again. Four and a half hours after the saline and each pyrogen administration blood samples were collected from the control and experimental pigeons. The total protein, gamma globulin, lysozyme, acute phase protein (Cp, CRP, Tf, ferritin, Alb) and selected trace element (Fe, Cu, Zn) concentrations were investigated. The obtained results showed the increase in the concentration of total protein, Cp, CRP and Tf in endotoxin fever resulting from LPS injection in pigeons. In contrast, the concentration of gamma globulins, ferritin and Alb were decreased in response to the first LPS injection. However, the consecutive injections of LPS caused a decrease in the concentration of total protein, CRP and Tf. In opposition to those results, a significant rise in the lysozyme and ferritin concentrations was observed. On the other hand, the first LPS injection caused a decline in the iron and zinc concentrations which remaining lower than the control values following repeated administration of LPS. On the contrary, the copper concentration increased successively in response to the next LPS injections.
2
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Changes of U937 cell viability induced by stress factors - possible role of calmodulin

100%
Wojcik-Piotrowicz K., Kaszuba-Zwoinska J., Rokita E., Nowak B., Thor P.
Journal of Physiology and Pharmacology
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2017
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tom 68
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nr 4
3

Investigation of inorganic deposits in selected organic matrices

86%
Cichocki T., Heck D., Jarczyk L., Rokita E., Strzalkowski A., Sych M.
Folia Histochemica et Cytobiologica
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1988
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tom 26
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nr 4
4
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Pulsating electromagnetic field stimulation prevents cell death of puromycin treated U937 cell line

72%
Kaszuba-Zwoinska J., Wojcik K., Bereta M., Ziomber A., Pierzchalski P., Rokita E., Marcinkiewicz J., Zaraska W., Thor P.
Journal of Physiology and Pharmacology
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2010
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tom 61
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nr 2
Aim of study was to verify whether pulsating electromagnetic field (PEMF) can affect cancer cells proliferation and death. U937 human lymphoid cell line at densities starting from 1x106 cells/ml to 0.0625x106 cells/ml, were exposed to a pulsating magnetic field 50Hz, 45±5 mT three times for 3 h per each stimulation with 24 h intervals. Proliferation has been studied by counting number of cells stimulated and non-stimulated by PEMF during four days of cultivation. viability of cells was analyzed by APC labeled Annexin V and 7-AAD (7-amino-actinomycin D) dye binding and flow cytometry. Growing densities of cells increase cell death in cultures of U937 cells. PEMF exposition decreased amount of cells only in higher densities. Measurement of Annexin V binding and 7-AAD dye incorporation has shown that density-induced cell death corresponds with decrease of proliferation activity. PEMF potentiated density-induced death both apoptosis and necrosis. The strongest influence of PEMF has been found for 1x106cells/ml and 0.5x106 cells/ml density. To eliminate density effect on cell death, for further studies density 0.25x106 cells/ml was chosen. Puromycin, a telomerase inhibitor, was used as a cell death inducer at concentration 100 µg/ml. Combined interaction of three doses of puromycin and three fold PEMF interaction resulted in a reduced of apoptosis by 24,7% and necrosis by 13%. PEMF protects U937 cells against puromycin- induced cell death. PEMF effects on the human lymphoid cell line depends upon cell density. Increased density induced cells death and on the other hand prevented cells death induced by puromycin.
5
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Magnetic field anti-inflammatory effects in Crohn's disease depends upon viability and cytokine profile of the immune competent cells

72%
Kaszuba-Zwoinska J., Ciecko-Michalska I., Madroszkiewicz D., Mach T., Slodowska-Hajduk Z., Rokita E., Zaraska W., Thor P.
Journal of Physiology and Pharmacology
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2008
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tom 59
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nr 1
AIM. We investigated effects of pulsating electromagnetic field (PEMF-50 Hz, 45 ± 5 mT) on viability and cytokine production by human peripheral blood mononuclear cells (PBMC) from healthy donors and from Crohn’s disease patients (CD). METHODS. The study was performed after activation of cells with phytohaemaglutinin (PHA) and lipopolisaccharide (LPS). Exposure of PBMC cultures to PEMF from both CD patients and from healthy donors decreased cell’s viability of about 10% and 5% (p>0,05) respectively. PEMF influence was most effective after threefold application. Susceptibility of PBMCs to magnetic field exposure differs among the stimulated (PHA, LPS) and not stimulated (NS) cells. Mitogen activated cells during cell division are most susceptible to induction of the cell death as a result of magnetic interaction, contrary PEMF exposure has minimal effect on non-diving PBMCs from CD patients and from controls. Decreased viability of the Crohn derived cells upon magnetic stimulation was accompanied by altered cytokines profile. Exposed and stimulated PBMCs from Crohn patients decreased IFN- proinflammatory and increased IL-10 anti-inflammatory cytokine production. The electromagnetically induced cell death could be an important step for non-invasive PEMF treatment in chronic inflammatory diseases.
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