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1

Modulation of plant nuclear matrix nucleolytic activity and specificity may result from interaction of nuclease-protein 14-3-3 complex with inner nuclear membrane lipids

100%
Lubocka J., Kozubek A.
Cellular and Molecular Biology Letters
|
1998
|
tom 03
|
nr 1
Plant cell nuclear matrix depleted in lipids exhibited approximately 50% lower endonucleolytic activity attributed to 32 kDa nuclease. Supplementation of delipidated matrix with phospholipids, sterols and glycolipids resulted in the recovery of nuclease activity. The extent of recovery is dependent upon the type of lipid used for reconstitution. The most effective (over 97%) in recovery of the nucleolytic activity were PC, PE, DGDG and stigmasterol. Some recovery is also observed when other natural amphiphilic molecules are studied (resorcinolic lipids). Recombinant plant protein 14-3-3 showed ability for direct interaction with lipid monolayers. The interaction was confirmed by analysis of the monolayer collected from the subphase after incubation with protein 14-3-3. Deletion of the 12 kDa N-terminal fragment of the protein 14-3-3 abolished its ability for binding to lipid monolayer. The results suggest that in vivo direct interaction of protein 14-3-3 with nuclear envelope lipids may participate in modulation of the nuclease 32 kDa activity. A postulated model of the interactions is discussed.
2

Modulation of nuclear matrix nucleolitic activity by lipids

100%
Lubocka J., Kozubek A.
Cellular and Molecular Biology Letters
|
1997
|
tom 02
|
nr 2
3

Modulation of some nuclear matrix enzymatic activities by free fatty acids

80%
Lubocka J., Szopa J., Kozubek A.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 2
It was shown that two of main enzymatic activities of plant nucleus and nuclear matrix, namely RNA-polymerasic and DNA-nucleolytic are susceptible to modulation with free fatty acids. The effects observed were dependent to both fatty acid length and degree of unsaturation. In nuclei a stimulation of nuclease activity was observed whereas in matrices short chain fatty acids inhibited the studied activity. The effect of fatty acids on RNA-poIymerase was also different in nuclei and matrices. In nuclei all fatty acids studied inhibited polymerasic activity whereas in matrices short chain fatty acids stimulated this activity by up to 80% and the long chain fatty acids inhibited by up over 70%. The overall alteration of studied activities in nuclei and matrices by unsaturated fatty acids was similar. Nucleolytic activity was stronger inhibited and polymerasic activity was stimulated when the effects of linoleic and linolenic acids were studied. The results suggest possible importance of lipid component in nuclear matrix biological function.
4

Modification of the lipid component modulates nuclear matrix nucleolytic activity

80%
Lubocka J., Szopa J., Kozubek A.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 2
It was shown that lipid composition of plant nuclear matrix depends on procedure of its isolation. The matrix isolated with the use of lithium diodosalicylate (LiS) differs in its lipid composition from the preparation isolated with the use of nonionic detergent (Triton X-100). It was also shown that the nucleolytic activity of the matrix is related to its lipid component. Matrix depleted in lipids loses half of its nucleolytic activity which is recovered after supplementation with previously extracted lipids. The extent of recovery of the nucleolytic activity is also dependent on the presence of residual DNA in matrix preparation. The recoveries of nucleolytic activities were higher in matrices not depleted in their DNA content.
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