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1

Auxins as one of the factors of plant growth improvement by plant growth promoting rhizobacteria

100%
Ahmed A., Hasnain S.
Polish Journal of Microbiology
|
2014
|
tom 63
|
nr 3
Plant growth promoting rhizobacteria (PGPR) promote plant growth by various mechanisms such as phytohormone production, enhanced water and nutrient uptake, improved nitrogen availability in the soil, production of ACC-deaminase for ethylene breakdown, phosphate solubilization, siderophore production etc. Microbial auxin production is the major factor not only responsible for strengthening the plant-microbe relationship but it also promotes plant growth and development in a positive manner. Thus, bacterial auxin production potential can be exploited for plant growth improvement that may be effective in reducing the hazardous effects of chemical fertilizers on the ecosystem used to obtain higher yields. The present review gives a better understanding of various factors and mechanisms involved in auxin production by PGPR that may be helpful in proper exploitation of these natural resources in a beneficial way.
2

Aeromonas punctata PNS-1: a promising candidate to change the root morphogenesis of Arabidopsis thaliana in MS and sand system

100%
Iqbal A., Hasnain S.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 03
A model system of sand, comprising Arabidopsis plants inoculated with Aeromonas punctata PNS-1 strain, was used to evaluate the bacterial effect in modulation of plant root structure at second-order lateral root level. In MS media, the root morphogenesis was changed only at first-order lateral root level when inoculated with PNS-1 strain. Inoculation with PNS-1 strain was subjected to significant (P\0.01) increase in primary root length and lateral root density in both MS and sand system. However, this strain modulated the root structure in the sand environment in a complex manner that may be helpful for incitation of the plant–microbe interaction close to natural environment. In order to determine whether this change in root morphology was due to bacterial auxin, Arabidopsis transgenic line (DR5:GUS) was used to reveal the change in homeostasis of endogenous auxin. In PNS-1 inoculated transgenic seedlings of Arabidopsis plant (DR5:GUS), endogenous auxin in primary root apices and lateral roots was enhanced. For confirmation, PNS-1 was evaluated for auxin production in vitro, showed an increase in auxin production after supplementation of L-tryptophan. The presence of ACC deaminase activity in PNS-1 showed its possible involvement in primary root elongation. In the present study Aeromonas punctata PNS-1 is the potential candidate for triggering the change in root morphogenesis of Arabidopsis thaliana with the involvement of auxin and ACC deaminase production.
3

Purification and characterization of an extracellular protease from Bacillus subtilis EAG-2 strain isolated from ornamental plant nursery

100%
Ghafoor A., Hasnain S.
Polish Journal of Microbiology
|
2010
|
tom 59
|
nr 2
107-112
Bacillus subtilis EAG-2 strain isolated from an ornamental plant nursery produced a highly active extracellular protease. It was purified to apparent homogeneity by successive purification steps. The SDS-gel of purified protease revealed a single band of 27 KDa on 10% polyacrylamide gel. Proteolytic activity was confirmed by using two different zymographic methods. Interestingly, the enzyme showed two clear activity bands in both cases. The optimum proteolysis for this protease was observed at pH 8.5 and 65°C. The enzyme was highly stable up to 80% after 30-50°C for 60 minutes. It also remained stable at pH 6.5-9.0 after 4 hours of incubation at 37°C. Its activity was reduced to 16% and 25% by PMSF and APMSF which indicates its relation to serine proteases. An increase in activity was noticed in the presence of Ca⁺², Zn⁺² and Ba⁺². On the other hand, it worked effectively with different natural substrates. Hence EAG-2 protease might be a useful contribution to the enzyme industry in Pakistan based upon its distinctive properties.
4

Occupational noise exposure may induce oxidative DNA damage

100%
Nawaz S. K., Hasnain S.
Polish Journal of Environmental Studies
|
2013
|
tom 22
|
nr 5
Occupational noise enhances the risk of several cardiovascular and non-cardiovascular diseases. The reason behind the drastic effects of noise exposure is the excessive generation of reactive oxygen species molecules. The present study investigates oxidative stress due to occupational noise exposure in 192 male smoking workers exposed to normal noise (sound level ≤80 dBA), low noise (sound level 81-94 dBA), and loud noise (sound level ≥95 dBA). University-aged volunteers were selected for the normal sound exposed group. Drivers and conductors were selected for the low-noise exposed group, and workers of power loom factories were chosen for the high-noise exposed group. Oxidative stress was estimated using 8 OHdG as a biomarker for oxidative DNA damage. Serum aldosterone and serum cortisol level was estimated using the enzyme immunoassay method. Results indicated that 8 OHdG level was significantly different in different exposure groups. It was highest in the low-noise exposed group (0.370±0.017 ng/ml) and lowest in the normal-sound exposed group (0.22±0.01 ng/ml). Level of 8 OHdG in the high-noise exposed group was 0.29±0.00. There was no significant variation of aldosterone levels among different groups. Cortisol levels of both noise groups was higher than that of the normal sound group. It can be concluded that noise exposure induces stress in the Pakistani population. This stress leads to oxidative DNA damage.
5

PCR-based screening approach: a rapid method to detect the biosynthetic potential of antimicrobials in actinobacterial strains

71%
Noureen N., Cheema M. T., Anwar S., Hasnain S., Sajid I.
Polish Journal of Microbiology
|
2020
|
tom 69
|
nr 2
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