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Disruption of Trichoderma reesei gene encoding protein O-mannosyl-transferase I results in a decrease of the enzyme activity and alteration of cell wall composition

100%

Gorka-Niec W., Pniewski M., Kania A., Perlinska-Lenart U., Palamarczyk G., Kruszewska J. S.

Acta Biochimica Polonica

2008

tom 55

nr 2

251-259

In fungi transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases encoded by pmt genes. Disruption of the pmt1gene in Trichodermacaused a significant decrease in the total activity of protein O-mannosyltransferases. Moreover, disruption of the pmt1gene also led to osmotic sensitivity of the strain, indicating an essential role of the PMTI protein activity for cell wall synthesis. At the same time, the strain was defective in septa formation, producing only half the number of septa per unit length of hypha compared with the wild type. Disruption of the pmt1gene decreased protein secretion but had no effect on glycosylation of secreted proteins, which suggests that PMTI protein O-mannosyltranferase does not take part in glycosylation of these proteins.

Alterations in protein secretion caused by metabolic engineering of glycosylation pathways in fungi

100%

Kruszewska J. S., Perlinska-Lenart U., Gorka-Niec W., Orlowski J., Zembek P., Palamarczyk G.

Acta Biochimica Polonica

2008

tom 55

nr 3

447-456

Due to its natural properties, Trichoderma reesei is commonly used in industry-scale production of secretory proteins. Since almost all secreted proteins are O-glycosylated, modulation of the activity of enzymes of the O-glycosylation pathway are likely to affect protein production and secretion or change the glycosylation pattern of the secreted proteins, altering their stability and biological activity. Understanding how the activation of different components of the O-glycosylation pathway influences the glycosylation pattern of proteins and their production and secretion could help in elucidating the mechanism of the regulation of these processes and should facilitate creation of engineered microorganisms producing high amounts of useful proteins. In this review we focus on data concerning Trichoderma, but also present some background information allowing comparison with other fungal species.

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Gorka-Niec W.

2 Kruszewska J. S.

2 Palamarczyk G.

2 Perlinska-Lenart U.

1 Kania A.

1 Orlowski J.

1 Pniewski M.

1 Zembek P.

2 2008

Wyniki wyszukiwania

Disruption of Trichoderma reesei gene encoding protein O-mannosyl-transferase I results in a decrease of the enzyme activity and alteration of cell wall composition

Alterations in protein secretion caused by metabolic engineering of glycosylation pathways in fungi