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Ship collision-avoidance trajectory planning aims at searching for a theoretical safe-critical trajectory in accordance with COLREGs and good seamanship. In this paper, a novel optimal trajectory planning based on hybrid genetic algorithm is presented for ship collision avoidance in the open sea. The proposed formulation is established based on the theory of the Multiple Genetic Algorithm (MPGA) and Nonlinear Programming, which not only overcomes the inherent deficiency of the Genetic Algorithm (GA) for premature convergence, but also guarantees the practicality and consistency of the optimal trajectory. Meanwhile, the encounter type as well as the obligation of collision avoidance is determined according to COLREGs, which is then considered as the restricted condition for the operation of population initialization. Finally, this trajectory planning model is evaluated with a set of test cases simulating various traffic scenarios to demonstrate the feasibility and superiority of the optimal trajectory
As an environmental factor, light influences the physiological functions and secondary metabolism of plants. However, the role of light in cotton fiber development and pigment biosynthesis has not yet been thoroughly explored. In this study, ovules of green cotton were cultured in vitro under dark and light conditions, and fiber and ovule growth parameters as well as fiber carbohydrate and cellulose contents and the expression of genes related to fiber development were investigated to elucidate the effect of light on fiber development. In addition, to investigate the effect of light on fiber pigment biosynthesis, the fiber flavonoid content and related gene expression were determined. The results demonstrated that the fiber length and the expression levels of fiber elongation genes under light culture were significantly lower than under dark culture, however, the ovule and fiber weight were significantly higher than under dark culture. The fiber developed under light culture had higher carbohydrate concentrations and carbohydrate transformation rate than under dark culture. Additionally, light culture exhibited higher cellulose contents and expression levels of cellulose biosynthesis genes compared with dark culture. In contrast, the pattern of the effect of light on flavonoid biosynthesis differed from that for cellulose biosynthesis. At 10 DAC (days after culture) and 20 DAC, the flavonoid contents and the expression levels of genes related to flavonoid biosynthesis were lower than under dark culture. However, the flavonoid contents and gene expression levels observed at 30 DAC and 40 DAC were higher in the light culture than in the dark culture. These results suggested that light hindered fiber elongation, but promoted carbohydrate accumulation and carbohydrate transformation, which resulted in fiber weight gain and increased cellulose accumulation in fibers. In addition, light inhibited flavonoid biosynthesis at early stage of fiber development, but promoted it at later stages. These findings provide the basis for intensive study of fiber development and flavonoids biosynthesis in green cotton.
Under accelerated urbanization and high-intensity human activities, the natural wetlands distribution area (NWDA) in Minjiang River estuary faces the great challenge of a deteriorative urban thermal environment. This work aims to analyze the impact of land use/land cover (LULC) on the urban thermal environment and model wetland surface temperature disturbance characteristics during the process of urbanization. The study utilized the following methodological steps: (1) mapping of LULC spatial and temporal distribution through photo interpretation; (2) applying the mono-window algorithm to obtain the spatiotemporal patterns of land surface temperature (LST); (3) examining the correlation between LST and different LULC classes, normalized difference vegetation index (NDVI), normalized difference moisture index (NDMI), and normalized difference impervious surface index (NDISI); and (4) exploring wetland surface temperature characteristics based on profile analysis and regression models. The results showed that the LST pattern depended on the LULC distribution; the high LST zones were mainly observed in the center of Fuzhou city, and the low LST zones were mainly observed in forest and river areas. Moreover, the urban thermal environment was influenced by both LULC classes and urban growth types. Finally, the positive relationship between LST and NDISI indicated an amplifying effect of the impervious surface for wetland surface temperature, while vegetation with high liquid water attenuated the regional high temperature. The obtained conclusions are expected to be beneficial in improving the design and management of the urban thermal environment.
Background. Arachidonic acid (ARA) is one of the three essential fatty acids, and it is important for human body to keep healthy and is widely used. At present, expensive materials such as glucose and yeast extract are generally reported to be optimal for ARA production. A new cost-effective fermentation process including cheaper material for ARA production is of great significance. Material and methods. Feasibility of using com meal and powdered soybean for fungal growth and lipid accumulation was evaluated by means of single factor test. N-hexadecane concentration was optimized, and the effect of temperature on biomass and ARA content was examined. Results. Mortierella alpina made better use of the aforementioned material as carbon and nitrogen sources for both hyphae growth and ARA production compared with glucose and yeast extract. Maximal levels of 10.9 g/L ARA and 26.1 g/L total lipids were obtained when 66 g/L com meal, 54 g/L soybean meal and 6% (v/v) n-hexadecane were supplemented. A temperature-shift strategy involved three steps, namely, 30°C (3 days) - 25°C (4 days) - 20°C (4 days), which further improved ARA production by 24.7%. Conclusion. Several factors such as carbon and nitrogen sources, temperature and dissolved oxygen had great influence on biomass and microbial oil production. Mortierella alpina preferred com and soybean meal compared with glucose and yeast extract, which would surely alleviate the high cost of ARA production. Based on this study, the new process is both low cost and practicable.
The correct folding of proteins in the endoplasmic reticulum (ER) is vital for plant adaptation to adverse environmental conditions. The genes related to the ER stress response in maize are far from clear. In this study, a maize nuclear factor, NF-YC14, was identified in the regulation of the ER stress response. Overexpression of NF-YC14 improves plant ER stress tolerance and up-regulates the expression of downstream ER stress response genes. Moreover, NF-YC14 involves abscisic acid (ABA) signaling and overexpression of NF-YC14 confers plant hypersensitivity to ABA. Furthermore, overexpression of NF-YC14 enhances the expression of downstream ER stress response genes mediated by the ABA signaling pathway in Arabidopsis. These results indicate that NF-YC14 and ABA signaling regulate the expression of ER stress response genes and NF-YC14 links ER stress response to ABA signaling.
The present study focused on laccase production, characterization, and its involvement in chlorophyll decolorization. Extracellularlaccase, with the highest activity of 11 U/ml on day 8, was efficiently produced from Trametes hirsuta in 5 l bioreactor with optimized media comprising dual carbon sources, glucose and water hyacinth. A laccase was then purified from the supernatant to homogeneity with purification fold of 9.51 and recovery of 39.8% and an estimated molecular mass of 62 kDa by SDS-PAGE. The laccase showed activity at pH 2–6 and temperature 30–80°C and was relatively thermally stable at below 70°C and neutral pH. The laccase was applied to decolorize chlorophyll under different factors: temperature, pH, mediator, metal ions, and enzyme dosage. Other fungal laccases were also found to be able to degrade chlorophyll with rating from 52% to 88% following 1 h treatment with two laccase dosages (5 or 10 U/ml) in the absence of any other mediators at room temperature. These findings may be an important step in developing new, important, and commercially viable industrial applications for laccase enzymes.
Brown cotton is a kind of naturally colored cotton. Because of less processing and little dying, it is more friendlier to environment than white cotton. For brown cotton, pigment accumulation in fiber is one of the most important characteristics. In this study, we selected a brown fiber line and a white fiber cultivar to determine the factor that affects the pigmentation in brown fiber. Accordingly, fibers were collected to verify the presence of PAs by p-dimethylaminocinnamaldehyde (DMACA) and toluidine blue O (TBO) staining. The PAs content and related genes expressions were determined. As a result, there were obvious differences on the aspect of PAs synthesis in fiber between white cotton and brown cotton. For white fiber, the PAs content reached maximum at 5 DPA, and then gradually decreased to zero. But for brown fiber, the PAs content was increased from 5 to 15 DPA stage, and reached the maximum at the 15 DPA stage, then gradually decreased from 15 to 40 DPA stage. On the contrary, in white cotton, PAs were synthesized in the whole developmental stage from 5 to 40 DPA. And PAs content in brown fiber were far more than that in white fiber, which may be the reason why the brown pigment accumulated in brown fiber.
Brown cotton is a kind of naturally colored cotton which accumulates brown pigment in fiber. In the experiment, the variations of DNA methylation and genes expression were studied during the development processes from 5 DPA to 25 DPA for brown fiber. By using the methylation-sensitive amplified polymorphism technique, we choose 66 pairs of selective-amplification primers to assess the status and levels of cytosine methylation. The hemimethylation of the external cytosine and the full methylation of the internal cytosine were scored. As a result, with the development of fiber from 5 DPA to 25 DPA, the ratios of the external hemimethylation and internal full methylation were gradually increased. In detail, at the stages of 5, 10, 15 and 25 DPA, the percentages of external hemimethylation were 8.29, 8.81, 9.77 and 10.09 %, the ratios of internal full methylation were 17.91, 19.20, 20.02 and 20.78 %, respectively. The development of brown fiber triggered the increase of methylated loci in the whole genome. For further analysis, we used cDNA-AFLP protocol to identify the specially expressed genes for further analysis. Totally, 30 polymorphic transcription-derived fragments (TDFs) were isolated and sequenced. By homologous alignment analysis, 19 TDFs were found similar with genes from cotton. Among them, the homologous genes of TDF5, TDF6, TDF10, TDF12, TDF17, TDF22, TDF23 and TDF25 were all from fiber. Furthermore, 13 TDFs were found to be homologous with reported functional genes.
The rapid global urban growth poses a great risk to the ecosystem services which are vital to sustaining and fulfilling human life. As an essential part of Fuzhou eco-planning task in south-eastern China, we identified the priority conservation areas for ecosystem services to allow a sustainable urban expansion. We modeled and mapped five ecosystem services (carbon storage, timber production, water yield, water-quality improvement and sediment retention) using InVEST and proposed a definition of priority areas for the conservation of ecosystem services. Priority areas for individual ecosystem services showed that 24% of the region was needed to produce 26% of water yield. Between 6 and 17% of the region was required to conserve at least 20% of other four services, depending on the ecosystem service of interest. In addition, scenarios for multiple ecosystem services conservation were developed using the ordered weighted averaging (OWA) method, a multicriteria evaluation method, to take the tradeoffs among ecosystem services into account. The results showed that, along with the decrease of the OWA risk, the overall areas and the areas at both of the conservative levels dropped gradually. Furthermore, two efficiency indices were created to evaluate the performance of different OWA scenarios. Study results suggested that the efficiency of scenarios was associated with the conservation threshold and OWA risk, as well as the spatial correlation among ecosystem services. In sum, identifying priority areas for ecosystem services in a spatially explicit manner, and analyzing tradeoffs between them, can help make land use and natural resource decisions more effective and efficient.
Background: To provide the anatomic basis for the clinical application of the transpedicular screw fixation. Materials and methods: Thirty spine (C₂ –L₅) specimens were used. The width of the pedicle cortex and width of the pedicle medullary cavity (WPC and WPMC), and the height of the pedicle cortex and height of the pedicle medullary cavity (HPC and HPMC) were measured at the isthmus of the pedicle using computed tomography (CT) scanning. Results: Width of the pedicle medullary cavity changed in a three-dovetailed-saddle shape with four peaks and three valleys, namely C₂ (high), C₄’₅ (low), T₂ (high), T₄ (the lowest), T₁₂ (high), L₁ (low) and L₅ (the highest). HPMC of the cervical pedicle changed in a saddle shape, gradually increasing from C₅–L₅. WPC, WPMC, HPC and HPMC showed a regular change, respectively. In each segment, the superior border of the pedicle cortex had a nearly consistent thickness to the interior border within an identical pedicle, while the pedicle cortex thickness radio of the medial and lateral border was nearly 3:1 among the cervical pedicles, 2:1 among thoracic pedicles, and 1:1 among lumbar pedicles. Conclusions: Both HPMC and WPMC are the dominant factors for the choice of screw diameter, but HPMC should also be considered in C2 –T₁ pedicles, especially C₆ and C₇. Additionally, the screw for C₃₋₆ or T₄₋₆ pedicles should be about 3.0 mm in diameter. (Folia Morphol 2019; 78, 3: 476–480)
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