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DNA replication reaction in Xenopus cell-free system is suppressed by high pressure

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Takahashi H., Yamaguchi T., Koga M., Kageura H., Terada S.

Cellular and Molecular Biology Letters

2004

tom 09

nr 3

Previously, we demonstrated that when mouse erythroleukemia cells are exposed to a pressure of 80 MPa, the cell-cycle progression of S-phase cells is retarded. To examine the effects of high pressure on DNA replication, we used a Xenopus cell-free system. From cell-cycle progression of sperm nuclei, it was found that sperm nuclei are stable to a pressure of 80 MPa, whereas egg extracts are susceptible to high pressure. Similarly, biotin-16-dUTP was incorporated into 80 MPa-treated sperm nuclei in pressure-untreated extracts, but not into naive sperm nuclei in 80 MPa-treated extracts. These results indicate that DNA replication in Xenopus cell-free system is suppressed by the susceptibility of the extracts to a pressure of 80 MPa.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Kageura H.

1 Koga M.

1 Takahashi H.

1 Terada S.

1 Yamaguchi T.

1 2004

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DNA replication reaction in Xenopus cell-free system is suppressed by high pressure