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Use of cultured cells of mammal and insect origin to assess cytotoxic effects of the pesticide Chlorpyrifos

100%

Pollakova J., Pistl J., Kovalkovicova N., Csank T., Kocisova A., Legath J.

Polish Journal of Environmental Studies

2012

tom 21

nr 4

In the present study four different cell cultures, derived from rabbit kidney (RK13), rat, and murine liver (WBF344 and Hepa 1c1c7) and insect origin (Sf21) were used to examine the effects of chlorpyrifos. Sf21 cells were the most sensitive to chlorpyrifos, with significant suppression of their proliferative activity ranging from 10⁻¹-10⁻⁵ M. However, significant suppression of proliferative activity also was recorded in mammalian cell cultures Hepa 1c1c7 (10⁻¹-10⁻³ M), WBF344 (10⁻¹-10⁻² M), and RK13 ( 10⁻¹ M). A cytopathic effect and LDH leakage into the medium was observed in RK 13 ( 10⁻¹-10⁻³ M) > WBF344 and Hepa 1c1c7 cells ( 10⁻¹-10⁻² M) > Sf21 ( 10⁻¹ M) compared to solvent control. Our results indicate that chlorpyrifos exposure caused a species-dependent decrease in cell proliferation and cell membrane damage.

Impact of zinc sulfate exposition on viability, proliferation and cell cycle distribution of epithelial kidney cells

84%

Marcincakova D., Schusterova P., Mudronova D., Csank T., Falis M., Fedorova M., Marcincak S., Hus K. K., Legath J.

Polish Journal of Environmental Studies

2019

tom 28

nr 5

Zinc is an essential trace element effective at very low concentrations, but it is also an important environmental pollutant dangerous after excessive intake or exposure. The aim of this study was to evaluate in vitro nephrotoxicity of zinc sulfate heptahydrate ZnSO₄ × 7H₂O (1, 10, 50, 100, 200 mg/l) using rabbit epithelial kidney cells RK13 as the model cell line. The xCELLigence system (RTCA) for real-time monitoring of cell response and the end-point assays for determining metabolic activity (MTT test), cytotoxicity (LDH test), proliferation (BrdU test) and cell cycle analysis were compared. Exposure to zinc sulfate produced dose-dependent cytotoxicity. The inhibition concentration IC50 value for xCELLigence monitoring was 101.8 mg/l, for MTT test 135.9 mg/l and 197.4 mg/l for BrdU test. There was a significant correlation between used assays (p≤0.05) except for the LDH test. Based on mean of IC50 values, the effect of zinc sulfate at 150 mg/l on cell cycle was evaluated. We observed the accumulation of cells in S phase accompanied with the reduction of cells in the G0/G1 phase. In conclusion, the mean of our IC50 values for kidney cells is relatively high in comparison to the recommended as well as therapeutic daily doses.

Ograniczanie wyników

2 Polish Journal of Environmental Studies

2 Csank T.

2 Legath J.

1 Falis M.

1 Fedorova M.

1 Hus K. K.

1 Kocisova A.

1 Kovalkovicova N.

1 Marcincak S.

1 Marcincakova D.

1 Mudronova D.

1 Pistl J.

1 Pollakova J.

1 Schusterova P.

1 2019

1 2012

Wyniki wyszukiwania

Use of cultured cells of mammal and insect origin to assess cytotoxic effects of the pesticide Chlorpyrifos

Impact of zinc sulfate exposition on viability, proliferation and cell cycle distribution of epithelial kidney cells