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The purpose of the study was to evaluate the effects of intrathecal (IT) ketamine HCl anesthesia on clinical values and some haemodynamic and biochemical parameters in bitches. An IT ketamine (10 mg/kg) was administered to 30 bitches with a spinal needle (18-22 G) in the lumbosacral space. The haemodynamic parameters were monitored and some biochemical values were assessed (blood gase, oxygen-haemoglobin, and electrolyte levels). The length and depth of anaesthesia was determined with a pinprick test, touching to the ligamenta lata uteri and incision. Anaesthesia took effect in less than 1 min in all dogs and has lasted an average of 95.9 min. In spite of the fact that the dogs recovered completely from the effects of dissociative anaesthesia, the anaesthesia in the some extremities was observed to be continued for an average of 17 min longer. The use of IT ketamine HCl raised blood pressure and did not have a depressive effect on respiratory and cardiac functions. It was concluded that ketamine HCl could be an appropriate alternative for ovariohysterectomy operations in bitches when the quality of the anaesthesia and the prevention of bradycardia and hypotension are considered.
The aim of this study was to investigate paraoxonase (PON1) activity and total sialic acid to be measured for the first time in sheep infected naturally with foot and mouth disease, and their relationships with oxidative stress. A total of 30 Awassi sheep (aged between 2-4), which were healthy and infected with the foot and mouth disease virus (FMDV) were used in the study. Concentrations of paraoxonase activity (PON1), which is an important antioxidant against oxidative stress, high density lipoprotein (HDL) and total sialic acid (TSA), which has a critical role for immune system and is one of the significant indicators of cellular degeneration, were measured in serum samples drawn from animals. The total oxidant capacity (TOC) and total antioxidant capacity (TAC) were evaluated to determine the oxidative balance. It was observed that serum PON1 (P < 0.001) and HDL (P < 0.01) concentrations were significantly lower compared to the control group. TSA concentration was higher in the infected group (P < 0.001) compared to the healthy group. TOC was higher (P < 0.001) and TAC was lower (P < 0.01) in the infected group compared to the control group. Consequently, harmful effects of the infection of foot and mouth disease were shown as cause of oxidative cell damage and the high rate of sialic acid was produced depending on the degeneration during the infection.
The purpose of this study was to perform the clinical, histopathologic and biochemical evaluation of the effects of intraperitoneally (IP) administered dexpanthenol (dxp) on colonic anastomosis healing in rats. The study was conducted on a total of 28 rats divided into four groups comprising seven rats each. Group I was designated as the control group, group II as the dxp group, group III as the anastomosis group, and group IV as the anastomosis + dxp group. The groups were compared in terms of intestinal bursting pressure, adhesion formation, nitric oxide (NO) and malondialdehyde (MDA) levels, total antioxidant capacity (TAC) and total oxidant capacity (TOC) in blood and intestinal homogenates, as well as histopathologic findings. Dxp decreased adhesion formation (6 rats in group III and 4 rats in group IV). Mean bursting pressures were higher in the dxp groups than in the other groups (group II = 254.3 ± 42.1 mmHg, group IV = 109.3 ± 34.5 mmHg). Moreover, there was a remarkable decrease in the levels of NO and MDA and in blood oxidative stress parameters in the dxp groups. The results suggest that dxp increased intestinal bursting pressure by accelerating healing in the anastomosis line and decreased adhesion formation, positively affecting healing. Dexpanthenol, which was found to have positive effects in the experimental rat model, can be introduced into clinical practice.
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