The consequent effect of growth retardants: paclobutrazol – 0.1, 0.5, 1, 5 mg l⁻¹, flurprimidol – 0.1, 1, 5 mg l⁻¹, chlorocholine chloride (CCC) – 5, 50 mg l⁻¹ on the growth and development of Tibouchina urvilleana Cogn. in vitro was examined. Paclobutrazol at concentration of 1 or 5 mg l⁻¹ and flurprimidol at 5 mg l⁻¹ inhibited the growth of shoots significantly. It was noted that shoots originating from these media had thicker, straighter stems and shorter internodes. The stimulating influence of CCC at 5 mg l⁻¹ on the elongation of the main shoot was observed. Axillary shoots formation was the best when shoots were derived from the medium supplemented with flurprimidol 1 mg l⁻¹. Paclobutrazol at 0.5 and 1 mg l⁻¹ and flurprimidol at 5 mg l⁻¹ influenced the formation and fresh weight of roots increase, but flurprimidol was more effective. Growth retardants (except paclobutrazol at 5 mg l⁻¹) applied in the earlier passage stimulated elongation of roots.