Wyniki wyszukiwania

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Identifications and preliminary characteristics of several novel tobacco genes regulated by sulphur deficiency stress

100%

Lewandowska M., Wawrzynska A., Sirko A.

Biological Letters

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2005

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tom 42

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nr 2 Spec.Vol.

2

Using yeast two-hybrid system for identification of the proteins interacting with the product of a novel plant gene induced by sulphur deficiency stress

76%

Kaminska J., Lewandowska M., Wawrzynska A., Gaganidze D., Liszewska F., Sirko A.

Biological Letters

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2005

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tom 42

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nr 2 Spec.Vol.

3

Overexpression of genes involved in phytochelatin biosynthesis in Escherichia coli: effects on growth, cadmium accumulation and thiol level

76%

Wawrzynska A., Wawrzynski A., Gaganidze D., Kopera E., Piatek K., Bal W., Sirko A.

Acta Biochimica Polonica

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2005

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tom 52

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nr 1

In Escherichia coli, heterologous production of Schizosaccharomyces pombe phytochelatin synthase (PCS) along with overproduction of E. coli serine acetyltransferase (SAT) and gamma-glutamylcysteine synthase (gammaECS) was achieved and resulted in the accumulation of phytochelatins in bacterial cells. Overproduction of either gammaECS alone or simultaneous production of all three proteins in bacterial cells were accompanied by reduced growth rate in liquid cultures. Interestingly, bacteria overproducing either gammaECS or both SAT and gammaECS (with elevated level of gamma-glutamylcysteine but not of phytochelatins) were able to accumulate more cadmium per dry weight than the control. However, the most efficient cadmium accumulation was observed in bacteria with elevated levels of all three proteins: SAT, gammaECS and PCS. Therefore, “pushing” the entire pathway might be the most promising approach in modification of bacteria for potential bioremediation purposes because the level of intermediates, cysteine and glutathione, can limit the rate of production of phytochelatins. However, in such bacteria other metabolic process might become limiting for efficient growth.

Ograniczanie wyników

2 Biological Letters

1 Acta Biochimica Polonica

3 Sirko A.

3 Wawrzynska A.

2 Gaganidze D.

2 Lewandowska M.

1 Bal W.

1 Kaminska J.

1 Kopera E.

1 Liszewska F.

1 Piatek K.

1 Wawrzynski A.

3 2005

Identifications and preliminary characteristics of several novel tobacco genes regulated by sulphur deficiency stress

Using yeast two-hybrid system for identification of the proteins interacting with the product of a novel plant gene induced by sulphur deficiency stress

Overexpression of genes involved in phytochelatin biosynthesis in Escherichia coli: effects on growth, cadmium accumulation and thiol level