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FISH mapping in cattle (Bos taurus L.) is not yet out of fashion

100%

De Lorenzi L., Molteni L., Parma P.

Journal of Applied Genetics

2010

tom 51

nr 4

Physical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in species whose assembled genome sequences are available. However, in this work we evidence the existence of errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results indicate that FISH mapping in cattle is still useful.

Chromosomal assignment of R-spondin genes in the donkey (Equus asinus, 2n equals 62)

76%

De Lorenzi L., Genualdo V., Perucatti A., Pia Di Meo G., Molteni L., Iannuzzi L., Parma P.

Journal of Applied Genetics

2010

tom 51

nr 3

R-spondins constitute a recently discovered small family of growth factors, and the evidence of their role in several developmental pathways is growing fast. In this work we describe the chromosomal location of the four RSPO genes in the donkey. Using horse BACs, we localized RSIPO1 on EAS 5q23, RSPO2 on EAS 12q13, RSPO3 on EAS 24q26, and RSPO4 on EAS 15p13. Moreover, RSPO2, RSPO3, and RSPO4 are the first genes mapped on donkey chromosomes 12, 24, and 15, respectively.

Ograniczanie wyników

2 Journal of Applied Genetics

2 De Lorenzi L.

2 Molteni L.

2 Parma P.

1 Genualdo V.

1 Iannuzzi L.

1 Perucatti A.

1 Pia Di Meo G.

2 2010

Wyniki wyszukiwania

FISH mapping in cattle (Bos taurus L.) is not yet out of fashion

Chromosomal assignment of R-spondin genes in the donkey (Equus asinus, 2n equals 62)