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1

GHR gene polymorphism in breeding stocks of chinchillas

100%
Polasik D.
Electronic Journal of Polish Agricultural Universities. Series Animal Husbandry
|
2013
|
tom 16
|
nr 4
2

Analiza autentyczności produktów pochodzenia zwierzęcego z wykorzystaniem testów DNA

63%
Polasik D., Terman A.
Przegląd Hodowlany
|
2015
|
tom 83
|
nr 6
3

Analiza zaleznosci miedzy polimorfizmem genu receptora ryanodiny [RYR1] a niektorymi cechami uzytkowosci rozplodowej swin

51%
Kmiec M., Terman A., Kulig H., Polasik D.
Folia Universitatis Agriculturae Stetinensis. Zootechnica
|
2005
|
tom 47
81-87
4

Analiza wpływu polimorfizmu w locus ESR/PvuII na wybrane cechy ilościowe i jakościowe nasienia knurów

51%
Kmiec M., Polasik D., Terman A., Wojdak-Maksymiec K.
Prace i Materiały Zootechniczne. Zeszyt Specjalny
|
2004
|
nr 15
5

Markery genetyczne a użytkowość rozpłodowa świń

51%
Mucha A., Ropka-Molik K., Polasik D., Terman A.
Przegląd Hodowlany
|
2013
|
tom 81
|
nr 6
6

Analysis of influences in ESR/PvuII locus polymorphism on quantitative and qualitative traits of semen in boars

51%
Kmiec M., Terman A., Wojdak-Maksymiec K., Polasik D.
Animal Science Papers and Reports. Supplement
|
2004
|
tom 22
|
nr 3
7
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An analysis of PPARGC1A gene polymorphism in relation to carcass quality in PIC hybrid fatteners

51%
Polasik D., Glodek A., Rybarczyk A.
Acta Scientiarum Polonorum. Zootechnica
|
2013
|
tom 12
|
nr 4
The aim of this study was to determine the association between polymorphism located in exon 8 of PPARGC1A gene (Cys430Ser) and carcass quality in pigs. Experiment was carried out on 350 PIC hybrid fatteners. Polymorphism was analyzed using PCR-RFLP method. The frequency of genotypes was as follows: AA – 0.33, AT – 0.57, TT – 0.1, however alleles: A – 0.62, T – 0.38. In the analyzed population loss of Hardy-Weinberg equilibrium was observed (P ≤ 0.01). Statistical analysis showed that only one of the evaluated traits was associated with individual PPARGC1A genotypes. Cooling loss value for pig carcasses with TT genotype was statistically significant (P ≤ 0.05) higher than observed in those with AA and AT genotypes.
8

An analysis of MYH7 single nucleotide polymorphism (g.7:75667956G>A) in relation to growth and carcass traits in pigs

51%
Polasik D., Tyra M., Zak G., Terman A.
Journal of Animal and Feed Sciences
|
2018
|
tom 27
|
nr 4
The aim of this study was to evaluate MYH7 single nucleotide polymorphism (SNP) (NC_010449.4: g.7:75667956G>A) in relation to growth and carcass traits in pigs reared in Poland. Previous study has shown that g.7:75667956G>A substitution in pigs influences miR-208b and MYH7 expression, and is associated with proportion of muscle fibre types. The presented study was conducted on 582 pigs belonging to six breeds: Polish Landrace, Polish Large White, Puławska, Pietrain, Duroc and Hampshire. Statistical analysis (GLM procedure) was performed for first three breeds separately and for whole group together. Our study showed that investigated SNP was associated with test daily gain, average daily gain, age at slaughter and number of days in experiment (P ≤ 0.05 or P ≤ 0.01) in Polish Large White, Puławska and whole group. Among carcass traits MYH7 variants influenced mean backfat thickness from 5 measurements (P ≤ 0.05) in the same groups. We also noticed some associations for slaughter efficiency, weight of loin without backfat and skin, loin eye area and meat percentage (P ≤ 0.05 or P ≤ 0.01) but results were not consistent among breeds and whole group. Obtained results indicate that MYH7 SNP could be used as a genetic marker for improvement of some growth traits and backfat thickness in pigs
9

Single nucleotide polymorphisms in exon 10 of the chinchilla growth hormone receptor [GHR] gene

51%
Polasik D., Kmiec M., Liefers S., Terman A.
Journal of Applied Genetics
|
2005
|
tom 46
|
nr 4
403-406
The aim of this study was to detect SNPs in exon 10 of the chinchilla growth hormone receptor gene (GHR) by comparative sequencing. Sixty females of the same breed (Standard) were analysed. Four new SNPs were identified, which cause 3 amino acid substitutions in the intracellular domain of the receptor: G/C at position 135 bp (in relation to the total sequence of exon 10) (gln/his), CAG/AAA at 352 bp and 354 bp (gln/lys), and C/A at 641 bp (thr/asn).
10
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Polimorfizm genu IGF-1 u bydła rasy polska holsztyńsko-fryzyjska

45%
Polasik D., Adamska P., Wojdak-Maksymiec K., Kmiec M., Terman A.
Acta Scientiarum Polonorum. Zootechnica
|
2010
|
tom 09
|
nr 4
Celem niniejszych badań była charakterystyka genetyczna stada krów na podstawie polimorfizmu w regionie promotorowym genu IGF-1 oraz ustalenie ewentualnych zależności pomiędzy wykrytym polimorfizmem a cechami użytkowości mlecznej. Badaniem objęto 185 krów rasy polska holsztyńsko-fryzyjska odmiany czarno-białej. Polimorfizm genu IGF-1 oznaczano metodą ACRS-PCR. W analizowanym stadzie stwierdzono następujące częstości alleli: T – 0,43, C – 0,57. Porównując obserwowane i oczekiwane częstości genotypów odnotowano, iż badana populacja nie znajdowała się w stanie równowagi genetycznej. Heterozygotyczność analizowanego stada wyniosła 0,64. Analizując cechy użytkowości mlecznej w zależności od poszczególnych genotypów stwierdzono, iż genotyp CC w większości przypadków powiązany był z wyższymi wartościami ocenianych cech, jednak różnice te nie zostały potwierdzone statystycznie.
11
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Analysis of the myostatin gene (MSTN) polymorphism in four breeds of horses

45%
Polasik D., Pikula R., Gawlik J., Ochman J., Terman A.
Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica
|
2015
|
tom 35
12
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Association of CAST and RYR1 genes polymorphism with carcass and meat quality in crossbreed pigs with a share of Pietrain breed

45%
Rybarczyk A., Terman A., Zak G., Kumalska M., Polasik D.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2016
|
tom 55[2]
Association of CAST and RYR1 genes polymorphism with carcass and meat quality in crossbreed pigs with a share of Pietrain breed. The aim of this study was to determine the effect of the calpastatin (CAST/TaqI) and ryanodine receptor (RYR1) genes polymorphism on carcass and meat quality traits in Pietrain crossbred pigs. The polymorphism in CAST and RYR1 genes was detected using the PCR-RFLP (Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments) method. Two alleles of CAST gene were identified–A(0.34) and B (0.66) and three genotypes–AA(0.21), AB (0.25) and BB (0.54). In relation to carcass and pork quality, no statistically significant differences were found between the CT and CC genotypes of RYR1 gene as well as between AA, AB and BB genotypes of CASTgene. In addition, no significant interaction was found between CAST/TaqI × RYR1 genotypes and all the analyzed carcass and meat quality traits.
13

Influence of selected factors upon the blood loss from the carcasses of pigs free of the stress susceptibility gene (RYR1T)

39%
Rybarczyk A., Karamucki T., Drozd R., Polasik D., Lupkowska A., Michalecka A.
Animal Science Papers and Reports
|
2015
|
tom 33
|
nr 2
The aim of the study was to determine the effect of road transport, lairage time, meat % and carcass weight, sex, and PPARGC1A gene polymorphism on the degree of blood loss after slaughter. The study was conducted on 350 hybrid finishers from three farms located at different distance from the meat processing plant, but with the same methods of keeping animals and dietary treatment.The degree of blood loss was measured in the obliquus internus abdominis muscle using the compressor method. The results showed that the degree of blood loss from carcasses was influence by the ambient temperature before slaughter, lairage time and the meat percentage in finishers.Finishers slaughtered without a pre-slaughter rest, with the shortest fasting time and the highest meat % of carcasses (>60%), were characterized by the lowest blood loss. In addition, blood loss was significantly related to ambient temperatures during the pre-slaughter handling, when pigs were transported for a distance of 232 km. Summarising, animal welfare proved to be significant for blood loss in pigs free of stress sensitivity gene (RYR1T), especially in those with high meat %.Appropriate transport conditions and a sufficiently long lairage time may help in obtaining higher post slaughter blood loss.
14

Effect of the polymorphism in GPX5 gene on reproductive performance traits in Large White x Landrace crossbreed sows

39%
Polasik D., Terman A., Kumalska M., Chomczynska O., Urbanski P., Zak G.
Animal Science Papers and Reports
|
2017
|
tom 35
|
nr 1
15
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FTO and PLIN2 genes polymorphism in relation to growth traits of pigs

39%
Polasik D., Kamionka E. M., Tyra M., Zagrobelny D., Zak G., Terman A.
Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica
|
2019
|
tom 49
|
nr 1(348)
16

Leptin gene (LEP) polymorphism in PIC hybrid fatteners free from stress susceptibility gene (RYR1T)

39%
Rybarczyk A., Kmiec M., Szaruga R., Polasik D., Terman A., Natalczyk-Szymkowska W.
Electronic Journal of Polish Agricultural Universities. Series Animal Husbandry
|
2010
|
tom 13
|
nr 3
17

Analysis of FSHB gene polymorphism in Polish Landrace and Polish Large White x Polish Landrace sows

39%
Polasik D., Kumalska M., Sawaragi Y., Zak G., Tyra M., Urbanski P., Terman A.
Electronic Journal of Polish Agricultural Universities. Series Animal Husbandry
|
2016
|
tom 19
|
nr 1
18
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Effect of carcass meatiness level on meat quality of pigs monomorphic at genes RYR1 and LEP

39%
Rybarczyk A., Kmiec M., Gardzielewska J., Karamucki T., Jakubowska M., Terman A., Polasik D.
Polish Journal of Food and Nutrition Sciences
|
2009
|
tom 59
|
nr 4
The studies were carried out on 114 carcasses of hybrid porkers after Camborough 22 sows and PIC 337 boars classified in classes of the EUROP system.The carcasses of PIC pigs free from stress susceptibility gene (CC/RYR1) and monomorphic at the leptin gene (TT T3469C and GG G2728A) classified into meatiness class S, E and U differed significantly in respect of meatiness and backfat thickness but did not differ significantly in respect of weight. Meat coming from the carcasses classified into class S contained significantly less intramuscular fat than that from the carcasses classified into class U, with its content being an optimum in all meatiness classes. No significant differences were found however in the content of dry matter, total protein and ash in meat from the carcasses classified into S, E and U classes. Meat from the carcasses classified into class S was characterised by a significantly larger drip loss than that from the carcasses classified into class E but no significant differences were observed between mean values of pH24 and pH48, water-holding capacity, thermal drip, water-soluble protein content and meat colour parameters. Despite the lack of significant differences between mean values of most meat quality traits examined, a higher frequency of PSE and partly PSE meat in carcasses with meatiness over 60% (class S) may point to an unfavourable effect of high meatiness on the quality of meat.
19
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Revision of the species complex Amidostomum acutum (Lundahl, 1848) (Nematoda: Amidostomatidae) by use of molecular techniques

39%
Kavatska K. M., Polasik D., Dzierzba E., Jedrzejczak M., Kalasinska E., Rzad I.
Annals of Parasitology
|
2015
|
tom 61
|
nr 1
The aim of the work is to confirm the species differentiation of the nematodes of the Amidostomatidae family: Amidostomoides acutum (Lundahl, 1848) Lomakin, 1991; Amidostomoides monodon (Linstow, 1882) Lomakin, 1991, and Amidostomoides petrovi (Shakhtahtinskaya, 1956) Lomakin, 1991, which still are used in the parasitological literature as synonyms of Amidostomum acutum (Lundahl, 1848). The research material consisted of nematodes isolated from gizzards of dabbling ducks from the north-west of Poland. To confirm the species differentiation, DNA from the nematodes was isolated and approximately 630bp of the 28S rRNA gene were sequenced. The obtained DNA sequences were tabulated and then phylogenetic analysis were conducted using the UPGMA method. The results of the research distinctly diversify the nematodes of the genus Amidostomoides at the DNA level, which together with morphological and ecological differences among them (hosts from different systematic groups) enables to classify them into the separate species.
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