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This study proposes an integrated method that simulates and optimizes groundwater design and management in combination with goal programming, which establishes the equilibrium between technical and environmental constraints in a pump-and-treat system. This method is applied to a petroleum-contaminated site in Western Canada to identify optimal remediation strategies given a set of remediation scenarios. The significant influential factors are remediation duration, standard concentration levels, and total pumping rate. Results indicate that goal programming can greatly enhance the remediation effect under low contaminant concentrations. In the pump-and-treat system, wells I2, E1, and E3 are the dominant components, whereas wells M7 and M5 are sensitive to variations in the identified influential factors. These wells must therefore be monitored intentionally. Moreover, these factors influence one another in interaction. Thus, high total pumping rates do not always generate favorable outcomes, and a long remediation period is unnecessary. In conclusion, the three identified factors should be spontaneously considered in the general goal-programming framework.
This study proposes an inexact sequential response planning (ISRP) approach for floodplain management on the basis of two-stage stochastic programming techniques. It can facilitate rapid response decision making for upcoming severe flood disasters by efficiently operating available multiple control measures in a quantified manner. The case study considers a floodplain management problem where decision makers want to obtain a cost-effective combination of multiple floodplain management policies (i.e. constructing hydraulic engineering, flood diversion) for coping with the upcoming flooding disasters with various severity levels. Optimal management strategies obtained from ISRP are analyzed, following by the demonstration of extending potentials.
Greenhouse gas (GHG) emissions from municipal solid waste (MSW) management significantly contribute to high global warming potential (GWP). However, most studies have failed to facilitate identifying MSW management schemes capable of comprehensively meeting the goals from decision-makers at different hierarchical levels under uncertainties. This study develops an inexact bi-level linear programming (IBLP) model for collaborative control of GHG emissions and waste management in Beijing: MGU-MCL. The MGU-MCL model implies a leader-follower decision process, with the environmental sector providing the upper-level objective and the local authority dominating the lower-level objective. Then, an interactive fuzzy possiblistic approach is introduced to represent the satisfactory degrees of different decision-making levels. Results show that the MGU-MCL model decisions would reduce GHG emissions by about 9%, but increase management costs by 4% compared with the decisions from conventional models; the contribution of the landfill facilities to GHG emissions would be predominant, especially methane emissions; while the composting and incineration facilities would account for a large proportion of management cost. Further comparative analysis among the bi-level and single-level models indicates that the bi-level model could provide coordinated schemes under an integrated consideration of economic efficiency and environmental impact.
This study presents the effect of metal-enriched rice on concentrations of Pb, Cd, Zn, and Cu in the tissues of chicken. The experimental group of chicken (fed with contaminated rice) accumulated high concentrations of Pb (1.00, 0.73, 0.52, 0.99, and 13.8 mg/kg dw in kidney, liver, muscle, blood and feather, respectively), being 2, 1.2, 3.5, 3, and 3.5 times the corresponding tissue Pb in the control group of chicken. Liver seemed to be the primary tissue for Cd accumulation. High concentrations of Pb and Cu were found in feathers compared to other tissues. Lead concentrations in the muscle and liver of the experimental group and liver Cd concentrations in both experimental and control groups exceeded the maximum permissible limits of Pb and Cd in foods of PR China, respectively. The results indicated that there were significant effects of metalenriched rice on Pb and Cd accumulations in chicken, and this dietary exposure pathway poses a potential health risk to local residents.
Background: Mishandling of intracellular chloride (Cl−) concentration ([Cl−]i) in cerebrovascular smooth muscle cells is implicated in several pathological processes, including hyperplasia and remodeling. We investigated the effects of ClC-2-mediated Cl− efflux on the proliferation of human brain vascular smooth muscle cells (HBVSMCs) induced by angiotensin II (AngII). Methods: Cell proliferation and motility were determined using the CCK-8, bromodeoxyuridine staining, wound healing and invasion assays. ClC-2, PCNA, Ki67, survivin and cyclin D1 expression, and β-catenin and GSK-3β phosphorylation were examined using western blotting. Histological analyses were performed using hematoxylin and eosin staining and α-SMA staining. Results: Our results showed that AngII-induced HBVSMC proliferation was accompanied by a decrease in [Cl− ]i and an increase in ClC-2 expression. Inhibition of ClC-2 by siRNA prevented AngII from inducing the efflux of Cl− . AngII-induced HBVSMC proliferation, migration and invasion were significantly attenuated by ClC-2 downregulation. The inhibitory effects of ClC-2 knockout on HBVSMC proliferation and motility were associated with inactivation of the Wnt/β-catenin signaling pathway, as evidenced by inhibition of β-catenin phosphorylation and nuclear translocation, and decrease of GSK-3β phosphorylation and survivin and cyclin D1 expression. Recombinant Wnt3a treatment markedly reversed the effect of ClC-2 knockdown on HBVSMC viability. An in vivo study revealed that knockdown of ClC-2 with shRNA adenovirus ameliorated basilar artery remodeling by inhibiting Wnt/β-catenin signaling in AngII-treated mice. Conclusion: This study demonstrates that blocking ClC-2-mediated Cl− efflux inhibits AngII-induced cerebrovascular smooth muscle cell proliferation and migration by inhibiting the Wnt/β-catenin pathway. Our data indicate that downregulation of ClC-2 may be a viable strategy in the prevention of hyperplasia and remodeling of cerebrovascular smooth muscle cells.
To understand cold acclimation and cold tolerance in Vitis, we isolated a C-repeat binding factor 1 (CBF1) transcriptional activator from cold-sensitive Vitis vinifera ‘Manicure Finger’ and cold-tolerant wild Vitis amurensis. Under cold stress, the CBF1 transcript accumulation of V. amurensis increased, whereas that of V. vinifera showed no significant change. The transcript levels of VaCBF1 in the roots, stems, leaves, and petioles under cold stress were up-regulated in a time-dependent manner. The transcript level of VaCBF1 in the leaves was induced by salinity stress or by exogenous abscisic acid and salicylic acid. The presence of the cis-elements MBS, MYB, and MYC in the VaCBF1 promoter suggests that this promoter is a component of the CBF transduction pathway, which is involved in plant response to cold stress. The overexpression of VaCBF1 increased the cold tolerance of transgenic tobacco at −4 °C. The transcript level of the downstream target gene NtERD10D appeared in the transgenic lines under normal conditions, whereas that of NtERD10D and NtDREB3 improved under low temperature. We suggest that VaCBF1 enhances stress tolerance by increasing antioxidant activities and promoting downstream target gene expression.
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