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The aim of the study was to assess the frequency of infectious BLV, BHV-1 and BVD-MD in cattle and estimate the degree of interaction between those viruses. The investigations were carried out in 3 herds of calves from identical industrial sheds. Antibodies against BLV were found in 51.6%, against BHV-1 in 39.9%, and an antibody against BVD-MD in 32.6%. The study showed a simple correlation between prevalence of antibodies against BLV and BHV-1 which means that as the percentage of infected animals with BLV increased, so did percentage of animals infected with BHV-1. The results suggest the possible coexistence of BLV and BHV-1 infection in cattle.
Blood was collected from bison that were selected between 1991 and 2001 for poor body condition, cachexia, lameness and balanoposthitis. Sera were tested for antibodies to bovine herpes virus type 1 (BHV-1), bovine viral diarrhoea virus (BVDV), foot-and-mouth disease virus (FMDV), parainfluenza virus type 3 (PI-3), Brucella abortus, Chlamydophila abortus, Coxiella burnetii, and Leptospira interrogans. The results of serological tests showed a prevalence of low titers of a serological reaction to Chlamydophila abortus (45.1%), bovine viral diarrhoea virus (29.5%), Leptospira interrogans (21.3%) and parainfluenza virus type 3 (13.9%). There were differences in the prevalence of antibodies to Ch. abortus between female and male bison. Futhermore, a relationship between age and antibodies to BVDV was observed in older bison. These results suggest that there is some transmission of bacterial and viral pathogens occurring between domestic and wild ruminants grazing in the same pastures in the peripherial areas of Białowieża Primeval Forest.
The aim of the study was to determine the etiological factors of an outbreak of cattle illness with symptoms nervous disorders. The disease appeared simultaneously in adult animals on three dairy farms between August and October. The majority of sick animals was after parturition and had high milk production. The diagnosis gene was found through clinical anatomopathology, histopathology and bacteriological examinations of the animals, as well as molecular, serological, virusological and chemical tests. L. monocytogenes was isolated from the brain tissue of the dead animals. Restriction analysis (PCR-RFLP) of the DNA fragment of the hly A gene from L. monocytogenes facilitated differentiation between the virulent and control strains. It was found that the copper and selenium levels in the cows' serum were reduced significantly. Additional examinations indicated the low content of these minerals in the forage.
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