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1

Charakterystyka pierwszego izolowanego w Polsce fermentujacego sorbitol werotoksycznego szczepu Escherichia coli O157

100%
Jakubczak A., Szych J., Januszkiewicz K.
Medycyna Doświadczalna i Mikrobiologia
|
2008
|
tom 60
|
nr 3
173-181
2

Molecular cloning and bioinformatic characterisation of FhPcW1, a new isoform of cathepsin L from adult Fasciola hepatica

88%
Jaros S., Zygner W., Januszkiewicz K., Jaros D., Wedrychowicz H.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
The aim of this study was to clone new secretory antigen of Fasciola hepatica and to predict its availability for immune system. The new cathepsin L - FhPcW1 (F. hepatico cysteine proteinase Warsaw 1), GenBank accession: EF407948, cDNA was cloned from adult F. hepatica flukes using RACE-PCR method. FhPcW1 is encoded by a 1,066 bp mRNA with a predicted open reading frame (ORF) of 326 amino acids (predicted pl = 5.41, m.w. = 37.137 kDa). Performed bioinformatic analysis included alignments of the nucleotide and amino acid sequences, the ExPASy (Expert Protein Analysis System) proteomics server of the Swiss Institute of Bioinformatics and National Center for Biotechnology Information. Performed analyses allowed to suppose that FhPcW1 is a secreted protein, which contains signal peptide, serine, threonine, tyrosine phosphorylation sites and four tyrosine sulfation sites, and does not contain glycosylation sites. The ORF corresponding to FhPcW1 exhibited strong similarity to previously cloned cathepsins L from the F. hepatico as well as F. gigantica. Predicted biochemical characteristics fits to the described before F. hepatica cathepsin Ls. Moreover, three dimensional model and MHC types ligation strength prediction were performed. Analysis of MHC type I and II peptide binding suggests that FhPcW1 may have significant immunogenic potential. The potential HLA II epitopes are situated at the outer surface of this protein. Thus, these epitopes seems to be available for immune response, especially for antibodies. This result may show that FhPcW1 seems to be a promising antigen for vaccination against F. hepatica.
3

Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages

88%
Baska P., Zawisowska-Deniziak A., Norbury L. J., Wisniewski M., Januszkiewicz K.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 1
4
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Content available

Molecular cloning of cDNAand preliminary analysis of cathepsin Llike enzyme from metacercariae of Fasciola hepatica

88%
Jaros S., Zawstowska A., Januszkiewicz K., Wisniewski M., Wedrychowicz H.
Wiadomości Parazytologiczne. Suplement
|
2007
|
tom 53
5

Excretory/secretory products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells

88%
Baska P., Norbury L. J., Wisniewski M., Januszkiewicz K., Wedrychowicz H.
Acta Parasitologica
|
2013
|
tom 58
|
nr 2
The liver fluke Fasciola hepatica infects a wide range of hosts, and has a considerable impact on the agriculture industry, mainly through infections of sheep and cattle. Further, human infection is now considered of public health importance and is hyperendemic in some regions. The fluke infection causes considerable damage to the hosts’ liver. However, the mechanisms of liver destruction have not yet been completely elucidated. In the present report we incubated a human liver cell line in the presence of either F. hepatica excretory/secretory material (FhES) or recombinant phosphoglycerate kinase (FhPGK). Dosedependent cytotoxicity in the presence of FhES was observed, indicating that FhES is capable of killing human hepatocytes, supporting a role for FhES in damaging host liver cells during infection; while treatment with a recombinant intracellular protein — FhPGK, had no impact on cell survival.
6
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Acomparison of protectivity of cDNAvaccination against Fasciola hepatica infections using a novel cathepsin L (FhPcW2) and previously tested cathepsin Ls

76%
Jaros S., Januszkiewicz K., Smaga-Kozlowska K., Wesolowska A., Zygner W., Wedrychowicz H.
Wiadomości Parazytologiczne. Suplement
|
2007
|
tom 53
7

Study of the local immune response in the peritoneal fluid and lymph nodes of rats vaccinated with cDNA encoding a phosphoglycerate kinase of Fasciola hepatica (cDNA-FhPGK)

64%
Wesolowska A., Zawistowska-Deniziak A., Norbury L. J., Wilkowski P., Januszkiewicz K., Pyziel A., Zygner W., Wedrychowicz H.
Annals of Parasitology
|
2013
|
tom 59
|
nr Suppl.
8

Immune responses in rats and sheep induced by a DNA vaccine containing the phosphoglycerate kinase gene of Fasciola hepatica and liver fluke infection

64%
Wesolowska A., Zawistowska-Deniziak A., Norbury L. J., Wilkowski P., Januszkiewicz K., Pyziel A. M., Zygner W., Wedrychowicz H.
Acta Parasitologica
|
2016
|
tom 61
|
nr 2
9

Evaluation of the cellular immune response of male and female rats vaccinated with cDNA encoding a phosphoglycerate kinase of Fasciola hepatica (cDNAFhPGK)

64%
Wesolowska A., Zawistowska-Deniziak A., Norbury L. J., Wilkowski P., Januszkiewicz K., Pyziel A., Zygner W., Wedrychowicz H.
Annals of Parasitology
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2013
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tom 59
|
nr Suppl.
10

Fasciola hepatica – the pilot study of in vitro assessing immune response against native and recombinant antigens of the fluke

64%
Baska P., Zawistowska-Deniziak A., Zdziarska A. M., Wasyl K., Wisniewski M., Cywinska A., Klockiewicz M., Januszkiewicz K., Wedrychowicz H.
Acta Parasitologica
|
2013
|
tom 58
|
nr 4
Fasciola hepatica is a liver fluke that infects 2.4 million of people and causes great economical loss in animal production. To date a 100% effective vaccine has not been developed and the disease is controlled by drug therapy. Great efforts are put into development of effective vaccine against parasite what is difficult since Fasciola spp. (like other helmints) during evolutionary process has developed sophisticated and efficient methods to evade immune response. During preliminary experiments it is convenient to use cell lines which are relatively cheap and allow for reproducible comparison of results between laboratories. We stimulated BOMA (bovine monocyte/macrophage cell line) and BOMAC (bovine macrophage cell line) with native or recombinant antigens of Fasciola hepatica and assessed IFN-γ, IL-4 and TNF-α level upon stimulation. We observed diminished secretion of proinflammatory TNF-α in LPS activated BOMA cells stimulated with Excretory/Secretory products of adult fluke (Fh-ES). We also observed greater changes in gene expression in LPS activated BOMA cells than in non activated BOMA cells upon stimulation using Fh-ES. The results show possibility of using cell lines for in vitro research of bovine immune response against liver fluke, although this model still requires validation and further characterization.
11

Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins

64%
Siwinska A. M., Baska P., Danilowicz-Luebert E., Januszkiewicz K., Dlugosz E., Wedrychowicz H., Cappello M., Wisniewski M.
Acta Parasitologica
|
2013
|
tom 58
|
nr 1
Ancylostoma ceylanicum belongs to a group of soil-transmitted helminths, which infect almost 576 mln people worldwide and are a major cause of anaemia and malnutrition. Upon contact with a permissive host, third-stage larvae (L3) residing in the environment become activated larvae (ssL3), a process associated with changes in the profile of gene expression. Ancylostoma secreted proteins (ASPs) are the major proteins secreted during larvae activation and play a crucial role in hookworm adaptation to parasitism. Here we report the cloning using RACE-PCR technique of three novel ASPs from the hookworm A. ceylanicum (Ace-asp-3, Ace-asp-4, and Ace-asp-5) and computational analysis of the protein sequences. All three proteins contain SCP (Sperm Coating Protein) domain characteristic for previously described ASP proteins. Real-time PCR analysis shows significant up-regulation of Ace-asp-3 and Ace-asp-5 expression in adult worms and correlated down-regulation in ssL3 larvae. On the other hand, expression of Ace-asp-4 was increased in ssL3 stages and decreased in adult parasites.
12

Evaluation of the immune response of male and female rats vaccinated with cDNA encoding a cysteine proteinase of Fasciola hepatica (FhPcW1)

64%
Wesolowska A., Norbury L. J., Januszkiewicz K., Jedlina L., Jaros S., Zawistowska-Deniziak A., Zygner W., Wedrychowicz H.
Acta Parasitologica
|
2013
|
tom 58
|
nr 2
Not only do males and females of many species vary in their responses to certain parasitic infections, but also to treatments such as vaccines. However, there are very few studies investigating differences among sexes following vaccination and infection. Here we demonstrate that female Sprague-Dawley rats vaccinated with cDNA encoding a recently discovered cysteine proteinase of Fasciola hepatica (FhPcW1) develop considerably lower liver fluke burdens after F. hepatica infection than their male counterparts. This is accompanied by differences in the course of their immune responses which involve different eosinophil and monocyte responses throughout the study as well as humoral responses. It is evident that host gender influences the outcome of parasitic infections after vaccination and research on both sexes should be considered when developing new treatments against parasites.
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