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Association of genetic variability within the Borrelia burgdorferi sensu lato with the ecology, epidemiology of Lyme borreliosis in Europe

100%

Derdakova M., Lencakova D.

Annals of Agricultural and Environmental Medicine

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2005

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tom 12

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nr 2

165-172

Lyme borreliosis (LB) represents the most common vector-borne zoonotic disease in the Northern Hemisphere. The infection is caused by the spirochetes of the Borrelia burgdorferi sensu lato (s.l.) complex which circulate between tick vectors and vertebrate reservoir hosts. The complex of Borrelia burgdorferi s.l. encompasses at least 12 species. Genetic variability within and between each species has a considerable impact on pathogenicity, clinical picture, diagnostic methods, transmission mechanisms and its ecology. The distribution of distinct genospecies varies with the different geographic area and over a time. In recent years, new molecular assays have been developed for direct detection and classification of different Borrelia strains. Profound studies of strain heterogeneity initiated a new approach to vaccine development and routine diagnosis of Lyme borreliosis in Europe. Although great progress has been made in characterization of the organism, the present knowledge of ecology and epidemiology of B. burgdorferi s.l. is still incomplete. Further information on the distribution of different Borrelia species and subspecies in their natural reservoir hosts and vectors is needed.

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Heterogeneity of Borrelia burgdorferi sensu lato and their reflection on immune response

61%

Stefancikova A., Derdakova M., Stepanova G., Pet'ko B., Szestakova E., Skardova I., Cislakova L.

Annals of Agricultural and Environmental Medicine

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2005

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tom 12

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nr 2

211-216

Geographically different strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto Ir 105, B. burgdorferi s.s. + B. afzelii V 123, B. garinii Ir 112 - isolates from eastern Slovakia, B. garinii K24 - isolate from western Slovakia and B. burgdorferi s.s. B 31 - American strain) were compared as antigens for serological study of Lyme borreliosis by IgG ELISA on a group of horses from eastern Slovakia. In a set of 101 horse serum samples, positivity with the use of Ir 105 strain was 53 (52.4%), with V 123 51 (51.49%), with Ir 112 48 (47.5%), with K 24 47 (46.5%) and with B 31 only 25 (24.7%). The seroprevalence between strains B 31 and Ir 105, B 31 and V 123, B31 and Ir112, B 31 and K 24 differed statistically significantly ( test chi2, p<0.05); however, the differences between strains Ir 105, V 123, Ir 112 and K 24 were insignificant. Consistency of positive and negative findings between American and Slovak strains ranged from 50.5-62.4%. Comparison of Slovak strains (Ir 105, V 123, Ir 112 and K 24) consistency of positive and negative findings was higher from 79.2-95.04% The highest consistency of findings was reached comparing strains Ir 112 and K 24, and the same high agreement of results was observed between the strains Ir 105 and V 123 and also Ir 112 and Ir 105. Higher consistency of findings of serologically examined horses with geographically close strains is in accordance with greater similarity of protein profiles of Slovak strains compared to the American strain.

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Simultaneous occurrence of Borrelia miyamotoi, Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus ticks in urban foci in Bratislava, Slovakia

61%

Vaculova T., Derdakova M., Spitalska E., Vaclav R., Chvostac M., Rusnakova Taragelova V.

Acta Parasitologica

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2019

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tom 64

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nr 1

4

Wild boar (Sus scrofa) — reservoir host of Toxoplasma gondii, Neospora caninum and Anaplasma phagocytophilum in Slovakia

61%

Reiterova K., Spilovska S., Blanarova L., Derdakova M., Cobadiova A., Hisira V.

Acta Parasitologica

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2016

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tom 61

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nr 2

5

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

61%

Cobadiova A., Reiterova K., Derdakova M., Spilovska S., Turcekova L., Hviscova I., Hisira V.

Acta Parasitologica

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2013

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tom 58

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nr 4

Parasitic diseases of livestock together with poor welfare conditions can negatively affect the health status and production of small ruminants. Protozoan parasites and tick-borne infectious agents are common threat of livestock including small ruminants mostly during the pasture season. Therefore the priority of the study was to analyse the circulation and presence of two protozoan parasites Toxoplasma gondii and Neospora caninum as well as tick-transmitted bacterium Anaplasma phagocytophilum in one selected goat farm in Eastern Slovakia. Throughout a three-year study period we have repeatedly screened the sera and blood of goats and dogs from monitored farm. In total, 343 blood serum samples from 116 goats were examined by ELISA. The mean seropositivity for T. gondii was 56.9% (66/116, CI (95%) = 48–66.0) and 15.5% (18/116, CI (95%) = 9.3–22.7) for N. caninum. The permanent occurrence of anti-Toxoplasma and anti-Neospora antibodies was detected in repeatedly examined goats during the whole monitored period. The presence of both parasites in the flock was analysed by PCR. DNA of T. gondii was confirmed in 12 out of 25 Toxoplasma-seropositive goats and N. caninum in 14 samples out of 18 Neospora-seropositive animals; four goats were co-infected with both pathogens. The risk of endogenous transmission of both parasites was pursued by examination of 41 kid’s sera, where seropositivity for toxoplasmosis was 31.7% and for neosporosis 14.6%. In dogs 61.1% seropositivity for T. gondii and 38.9% for N. caninum was found, however, their faeces were negative for coccidian oocysts. Eight out of 108 tested animals were infected with A. phagocytophilum, the causative agent of tick-borne fever. Seven of them were simultaneously infected with T. gondii and A. phagocytophilum, out of which four goats were concurrently infected with all three pathogens.

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Molecular evidence for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Eastern Slovakia

61%

Derdakova M., Halanova M., Stanko M., Stefancikova A., Cislakova L., Petko B.

Annals of Agricultural and Environmental Medicine

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2003

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tom 10

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nr 2

7

Anti-Borrelia antibodies in rodents: important hosts in ecology of Lyme disease

51%

Stefancikova A., Bhide M., Pet'ko B., Stanko M., Mosansky L., Fricova J., Derdakova M., Travnicek M.

Annals of Agricultural and Environmental Medicine

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2004

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tom 11

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nr 2

Data presented in this study focuses on the presence of anti-Borrelia antibodies in small mammals from Eastern Slovakia during 2000-2003. The total seropositivity observed was 18.78% in rodents. Amongst all species, the total seroprevalence in Apodemus flavicolis was the highest (20.87%), followed by Apodemus agrarius (19.58%) and Clethrionomys glareolus (11.11%). However, the prevalence in Apodemus flavicolis during the year 2000-2001 was higher (26.72%), which reduced to 10.60% in 2002-2003. To compare the year range of seroprevalence in other small mammals was not feasible due to the small sample number. Area-wise distribution of anti-Borrelia antibodies was even (18.75% to 20%) in this study, except in the Boťany province (0%). This confirms the equal distribution of Borrelia spirochetes in the other 3 localities. Prevalence of anti-Borrelia antibodies during summer was significantly higher than during autumn and early spring. The overall study also reviews the importance of small mammals in Lyme disease ecology.

Ograniczanie wyników

Association of genetic variability within the Borrelia burgdorferi sensu lato with the ecology, epidemiology of Lyme borreliosis in Europe

Heterogeneity of Borrelia burgdorferi sensu lato and their reflection on immune response

Simultaneous occurrence of Borrelia miyamotoi, Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus ticks in urban foci in Bratislava, Slovakia

Wild boar (Sus scrofa) — reservoir host of Toxoplasma gondii, Neospora caninum and Anaplasma phagocytophilum in Slovakia

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

Molecular evidence for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Eastern Slovakia

Anti-Borrelia antibodies in rodents: important hosts in ecology of Lyme disease