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Molecular characterization and detection of enterotoxins, methicillin resistance genes and antimicrobial resistance of Staphylococcus aureus from fish and ground beef

100%
Arslan S., Ozdemir F.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 1
A total of 120 samples including 40 freshwaterfish(Oncorhynchus mykiss), 40 seawater fish (Sparus aurata) and 40 ground beef samples were examined for the presence of Staphylococcus aureus. The isolates were identified using biochemical tests and a PCR for the species-specific fragment (Sa442) and thermonuclease gene (nucA). The presence of staphylococcal enterotoxin genes (sea, seb, sec, sed and see), toxin genes (eta, etb, tsst), methicillin resistance gene (mecA) and some phenotypic virulence factors was also tested. Genotypic characterization of the isolates was analyzed by PCR-RFLP of the coa gene. Overall, 36 (30%) meat samples were contaminated with S. aureus. Of the 36 isolates, 3 (8.3%) were found to be positive for enterotoxin genes. Only 1 isolate (5.9%) from ground beef had the sea gene. In addition, 1 (12.5%) of the freshwater fish and 1 (9.1%) of the seawater fish carried both the sea and sed genes. The presence of seb, sec, see, eta, etb and tsst was not detected among the isolates of S. aureus. The amplified coa gene revealed five different clusters. Seven and six distinct RFLP patterns were obtained with AluI and HaeIII digestion, respectively. All isolates were found to be positive for slime, hemolytic and DNase activity while 41.7% of them were beta-lactamase positive. The presence of methicillin resistance was neither detected by PCR nor the disk diffusion method. A total of 94.4% of the isolates were resistant to at least one antimicrobial while 44.4% of them were resistant to at least two or more antimicrobials.
2

Encapsulation of the essential oil of Salvia fruticosa with different wall materials

81%
Ozdemir F., Nadeem H. S., Torun M.
Polish Journal of Food and Nutrition Sciences
|
2011
|
tom 61
|
nr Suppl.1
3

Superoxide dismutase and peroxidase activities in drought sensitive and resistant barley [Hordeum vulgare L.] varieties

80%
Acar O., Turkan I., Ozdemir F.
Acta Physiologiae Plantarum
|
2001
|
tom 23
|
nr 3
4

Comparative effects of drought, salt, heavy metal and heat stresses on gamma-aminobutryric acid levels of sesame (Sesamum indicum L.)

61%
Bor M., Seckin B., Ozgur R., Yilmaz O., Ozdemir F., Turkan I.
Acta Physiologiae Plantarum
|
2009
|
tom 31
|
nr 3
Gamma-aminobutryric acid (GABA) accumulation in response to diverse stresses is well known in plants; however, the comparative effects of different environmental stresses on GABA accumulation was not addressed in the same plant system. Here, we investigated GABA accumulation comparatively in sesame (Sesamum indicum L. cv. Cumhuriyet) plant under drought, salt, heavy metal (Se) and high-temperature stresses. Plants were stressed by application of 5% PEG-6000, 150 mM NaCl, 100 µg g⁻¹ Se and high temperature (50°C for 2 h). Root and shoot growth reduced after PEG, NaCl, Se and high-temperature treatments. Among these, heavy metal treatment had the highest and earliest effect on growth. GABA accumulation could be related to stress perception rather than protection in sesame plant if we consider that the adverse effects of different abiotic stresses on growth were not elevated by GABA.
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