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The effects of different concentrations of Cr(VI) (1 µM, 10 µM, 100 µM) applied for 7, 14 or 21 days on initiation of high lipid peroxidation level (POL) and consequent changes in the enzymatic-antioxidant protective system and minimization of photosystem II (PSII) activity were studied in maize seedlings. Chromium (VI) caused an increase in the electrical conductivity of the cell membrane, and malondialdehyde (MDA) content (a peroxidation product) reflected peroxidation of membrane lipids leading to the loss of the membrane's selective permeability. It also induced distinct and significant changes in antioxidant enzyme activity. Versus the control, superoxide dismutase (SOD, EC 1.15.1.1.), catalase (CAT, EC 1.11.1.6.) and peroxidase (POD, EC 1.11.1.11.) activity in maize seedling roots and leaves was progressively enhanced by the different Cr(VI) doses and stress periods, except for decreases in SOD and POD activity in leaves exposed to 100 µM Cr(VI) for 21 days. The different Cr(VI) concentrations changed chlorophyll (chl) content differently. The 10 µM and 100 µM doses of Cr(VI) decreased the chl a/b ratio and quenched the chl a fluorescence emission spectra. These effects reflect disturbance of the structure, composition and function of the photosynthetic apparatus as well as PSII activity.
Long-term changes (2003-14) of water temperature, DO, pH, TN, NH4-N, TP, and CODMn were examined at eight sampling stations along the Liujiang River. Water quality parameters showed considerable spatial and temporal variability. Annual averages of those parameters were 22.1ºC, 7.8 mg/L, 7.58, 1.35 mg/L, 0.27 mg/L, 0.06 mg/L, and 1.7 mg/L, respectively. An increasing trend for TN/TP and a decreasing trend for pH were observed in all parts of the Liujiang. Pollution levels were generally higher in the lower Liujiang than in the upper and middle parts of the river due to the impact of urban sewage. All indicators reached level III water quality standards except TN, which suggests that the control of nitrogen emissions should be strengthened. Relatively high N/P ratios in the Liujiang contribute to a potential for phosphorus limitation of phytoplankton. The average concentration of Chlorophyll-a was 1.2 μg/L in 2014. The TLI index indicated that the eutrophication state of the Liujiang was mesotrophic, while the downstream water was polluted due to its nutrient inputs from agriculture and urban sources. The water quality of the river keeps well by comparison to other major rivers of the world, which provided the basis for urban development and river protection in Liuzhou City.
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