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1
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Serological survey of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Ehrlichia canis infections in rural and urban dogs in Central Italy

100%
Ebani V. V., Bertelloni F., Torracca B., Cerri D.
Annals of Agricultural and Environmental Medicine
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2014
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tom 21
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nr 4
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs

100%
Ebani V. V., Bertelloni F., Turchi B., Cerri D.
Annals of Agricultural and Environmental Medicine
|
2013
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tom 20
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nr 2
The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.
3
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Seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in Italian horses

100%
Ebani V. V., Bertelloni F., Pinzauti P., Cerri D.
Annals of Agricultural and Environmental Medicine
|
2012
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tom 19
|
nr 2
The aim of the study was to determine the seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in healthy horses living in 7 provinces of central Italy. In the period 2007-2009, sera from 386 horses were tested by microagglutination test (MAT) to detect antibodies to Leptospira spp., employing the following serovars as antigens: Bratislava, Ballum, Canicola, Icterohaemorrhagiae, Grippotyphosa, Hardjo, Pomona, Tarassovi. 3 animals were positive for the serovars Icterohaemorrhagiae, 2 to Bratislava, and 1 to Pomona, for a total 1.5% seroprevalence. All sera were examined by immunofluorence antibody test (IFAT) to reveal anti-B. burgdorferi s.l. antibodies. 94 (24.3%) horses were positive with antibody titres ranging from 1:64 to 1:1,024. The seroprevalence was significantly higher in >10 year-old horses compared to younger subjects. No significant differences in the mean seroprevalence were observed in the respective years. The total mean seroprevalence were strictly related to the environmental conditions of the areas in which the horses lived. No crossreactions between Leptospira and Borrelia were observed. This is the first serological survey on antibodies to B. burgdorferi s.l. in Italian horses.
4

Seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in Italian horses

100%
Ebani V. V., Bertelloni F., Pinzauti P., Cerri D.
Annals of Agricultural and Environmental Medicine
|
2012
|
tom 19
|
nr 2
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Antimicrobial resistance in Enterococcus spp. isolated from laying hens of backyard poultry flocks

76%
Bertelloni F., Salvadori C., Moni A., Cerri D., Mani P., Ebani V. V.
Annals of Agricultural and Environmental Medicine
|
2015
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tom 22
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nr 4
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