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This study evaluated agronomic traits of 111 genotypes of black gram Vigna mungo (L.) Hepper, mainly from Pakistan, to determine the extent of genetic diversity. Seed proteins were analyzed by SDS-PAGE. High genetic variance was observed for days to flowering, days to maturity, number of branches/plant, number of pods/plant, biomass/plant, grain yield/plant and harvest index, whereas low genetic variance was observed for pod length, seeds/pod and 100-seed weight in both years studied. SDS-PAGE of seed proteins showed low inter-accession diversity and no clear differentiation on the basis of origin or source. The genotypes in one cluster with similar agronomic characters did not necessarily belong to the same origin or source. The lack of a geographic pattern of germplasm collected from Pakistan was probably due to exchange of germplasm between neighbouring regions, or because germplasm represented progenies of the same ancestors. Clustering of advanced breeding lines along with an approved variety in one group revealed that only a portion of the genetic diversity has been exploited for improvement of black gram. It is suggested that the genetic base of cultivated black gram should be broadened by involving diverse parents in the breeding programme. Expansion of the genetic base for black gram breeding might be accomplished by systematic use of germplasm that differs in protein profiles and has better quantitative traits.
Aflatoxin B1 (AFB1 ) is a fungal metabolite and highly carcinogenic compound of category 1 according to the International Agency for Research on Cancer. In the liver AFB1 from contaminated feed is bioconverted into aflatoxin M1 and can be easily diffused to the animal milk. Provision of healthy milk for humans, particularly infants and adults, therefore, entails monitoring of AFB1 level in the feed for dairy animals. In the present study, AFB1 level was monitored in three different types of animal feed comprising commercially available animal feed, fresh fodder and leftover bread fed to dairy animals between October 2014 and September 2015. AFB1 was found in all collected feed samples at the amounts: 30.5%, 2.8% and 88.9% in commercial feed, fresh fodder and leftover bread samples, respectively. All these levels were over the EU permissible limits (5 μg · kg−1). Mean maximum levels of AFB1 were observed in all samples collected in the winter season, whereas the mean minimum levels – in the summer months. The results of the present study indicated that the leftover bread samples and commercial feed contain high levels of AFB1 , and so strict measures should be adopted to prevent dairy animal feed and at the same time the animal milk from aflatoxin contamination.
An enormous amount of waste material (sludge) generated from paper and pulp industries is either dumped or incinerated. However, this pulp is an enriched source of macronutrients and can be employed on agricultural land to improve its fertility. The current study was therefore performed to analyze phosphorus (P) and calcium (Ca) by flame photometer, nitrogen (N) by Kjeldahl method, and magnesium (Mg) by back titration from sludge. The application of sludge significantly increases macronutrients in the plants lady finger (Abelmoschus esculentus) and garden mint (Mentha sachalinensis).We applied primary (initial husk treatment) and secondary (after primary treatment) sludge to observe the growth of Abelmoschus esculentus and Mentha sachalinensis. However, primary sludge showed the highest concentration of nutrients, i.e., 96.5% P, 99.6% N, 86% Ca, and 0.008% Mg. The application of paper and pulp waste to the soil indicates that these bio-solids are good for soil fertility, biomass production, and plant growth; however, they are slightly less efficient than synthetic fertilizers. However, the synthetic fertilizers are probable candidates for holding heavy metals, so these bio-solids can be considered a better source for soil fertility.
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